Mon Jun 23 17:54:51 2008 ZZz ZZ zqa AM zxxx AM f30cryo cryoimaging asas
Mon Jun 30 14:19:46 2008 morphew 04/15 9:00 AM 4:45 PM tiltrotate rtimage tryps 4
Mon Jun 30 14:21:55 2008 schwartz 04/16 9:15 AM 5:30 PM f30cryo cryotomo QDs, Giardia 4
Mon Jun 30 14:22:49 2008 Schwartz 4/17 10:15 AM 10:45 AM standard serviceman new alignments cindi040708 new alignments
Mon Jun 30 14:23:48 2008 schwartz 04/17 10:45 AM 4:30 PM f30cryo cryoimaging Chloroplasts, Giardia
Mon Jun 30 14:24:30 2008 cedric 04/17 4:30 PM 10:00 PM f30cryo cryoimaging chloro
Mon Jun 30 14:25:14 2008 morphew 04/18 8:45 AM 2:30 PM tiltrotate rttomo tryps 4
Mon Jun 30 14:25:52 2008 schwartz 04/18 2:30 PM 4:15 PM standard rtimage eels couldn't figure out eels
Mon Jun 30 14:27:03 2008 cedric 04/22 8:15 AM 10:00 PM 914 cryotomo section chloro 4 motor makes nnoise in one direction
(right on the screen) at -50
Mon Jun 30 14:27:46 2008 morphew 04/23 1:30 PM 4:30 PM tiltrotate rttomo thylakoid 2
Mon Jun 30 14:28:37 2008 morphew 04/24 8:45 AM 5:00 PM tiltrotate rttomo thylakoid 7 stage movement at x=500 no good
Mon Jun 30 14:29:31 2008 schwartz 04/25 8:45 AM 4:45 PM 70 rttomo eels 1 stage locked up 1X, reset goniometer
Mon Jun 30 14:30:07 2008 morphew 04/28 9:00 AM 5:30 PM 70 rttomo u20s/melanoma 4
Mon Jun 30 14:30:44 2008 morphew 04/29 9:00 AM 4:00 PM 70 rttomo melanoma/3D-matrix 2
Mon Jun 30 14:31:14 2008 morphew 04/30 8:45 AM 12:00 PM tiltrotate rttomo thylakoid 4
Mon Jun 30 14:31:46 2008 schwartz 04/30 1:00 PM 4:30 PM standard rtimage eels
Mon Jun 30 14:32:25 2008 schwartz 05/01 9:00 AM 4:30 PM standard rtimage eels replaced std. o-ring
Mon Jun 30 14:32:58 2008 morphew 05/02 9:00 AM 2:00 PM tiltrotate rttomo thylakoid 5
Mon Jun 30 14:33:35 2008 Schwartz/Andy 05/02 2:00 PM 3:30 PM standard rtimage eels
eels
Mon Jun 30 14:34:23 2008 EOT 05/04 12:00 PM 6:30 PM tiltrotate rttomo u2os 3
Mon Jun 30 14:34:56 2008 EOT 05/06 8:30 AM 4:30 PM tiltrotate rttomo u2os 2
Mon Jun 30 14:35:44 2008 morphew 05/07 9:00 AM 3:30 PM tiltrotate rtimage AFP transfected COS 4 cindi changed o-ring on t/r rod
Mon Jun 30 14:36:30 2008 morphew 05/08 10:00 AM 1:00 PM tiltrotate rttomo AFP transfected COS 2
Mon Jun 30 14:37:32 2008 cedric 05/08 2:00 PM 5:30 PM f30cryo cryoimaging afp sections stage doesn't move with trackball:
10 times to move correctly...pain
in the ass
Mon Jun 30 14:38:08 2008 morphew 05/13 9:00 AM 4:30 PM tiltrotate rttomo melanoma 2
Mon Jun 30 14:38:37 2008 morphew 05/14 9:00 AM 2:00 PM tiltrotate rttomo melanoma/spc42 2
Mon Jun 30 14:39:12 2008 EOT 05/15 8:30 AM 2:00 PM tiltrotate rttomo cilia 4
Mon Jun 30 14:39:45 2008 schwartz 05/15 2:00 PM 2:30 PM standard serviceman reboot reboot computer to see if beam gets
brighter
Mon Jun 30 14:40:20 2008 morphew 05/16 9:00 AM 1:00 PM tiltrotate rttomo melanoma 4 rod sluggish on rotation
Mon Jun 30 14:41:09 2008 janet/odde 05/16 1:00 PM 5:00 PM tiltrotate rttomo ase1delete yeast spindles 4 rod sluggish on rotation
Mon Jun 30 14:41:48 2008 morphew 05/22 2:00 PM 4:00 PM tiltrotate rttomo olin's nuclei 1
Mon Jun 30 14:42:32 2008 Janet/Odde 05/29 8:10 AM 6:00 PM tiltrotate rttomo ase1delete yeast spindles 10 T/R motor not working at all
Mon Jun 30 14:43:01 2008 cedric 05/30 8:00 AM 5:00 PM 914 cryoimaging sections
Mon Jun 30 14:43:40 2008 power outage 05/30 10:20 PM 10:40 PM standard serviceman campus wide power outage
Mon Jun 30 14:44:20 2008 service 06/02 8:00 AM 1:00 PM standard serviceman cold start cold start, upper camera warmed,
refilled sf6
Mon Jun 30 14:44:51 2008 morphew 06/02 1:00 PM 6:15 PM 70 rttomo olins 4
Mon Jun 30 14:45:26 2008 morphew 06/03 8:45 AM 4:50 PM 70 rttomo olins 4
Mon Jun 30 14:46:02 2008 schwartz 06/04 10:00 AM 1:15 PM f30cryo cryoimaging testing vitreous grids not vitreous :(
Mon Jun 30 14:46:29 2008 morphew 06/04 1:00 PM 5:00 PM 70 rttomo olins 4
Mon Jun 30 14:46:51 2008 morphew 06/05 9:00 AM 4:00 PM 70 rttomo olins 6
Mon Jun 30 14:47:31 2008 Janet/Odde 06/06 8:30 AM 3:00 PM 70 rttomo ase1delete yeast spindles 6
Mon Jun 30 14:48:06 2008 DNM 06/06 3:30 PM 5:00 PM standard development knife edge on GIF
knife edge on GIF
Mon Jun 30 14:48:47 2008 cedric 06/10 12:30 PM 4:30 PM 914 cryoimaging sections from previous entry: video camera
stuck in
Mon Jun 30 14:49:25 2008 Janet/Winey 06/11 11:30 AM 4:45 PM 70 rttomo WT tet bbs 6
Mon Jun 30 14:49:57 2008 EOT 06/12 9:00 AM 3:30 PM 70 rttomo dynamin 1,2 KO 3
Mon Jun 30 14:50:31 2008 EOT 06/13 9:00 AM 2:30 PM 70 rttomo dynamin 1,2 KO 6
Mon Jun 30 14:51:00 2008 morphew 06/16 9:00 AM 5:00 PM 70 rttomo melanoma 2
Mon Jun 30 14:51:55 2008 morphew 06/19 9:00 AM 4:00 PM f20cryo cryoimaging BPV vacuum at 32 at start-up (also on
6/16)
Mon Jun 30 14:52:34 2008 DNM 06/23 8:30 AM 12:00 PM standard development testing testing rectangular distortion
field and skipping montage pieces
Mon Jun 30 14:53:06 2008 morphew 06/24 8:30 AM 1:00 PM f20cryo cryotomo BPV 1 vacuum high (29) at start-up
Mon Jun 30 14:53:43 2008 morphew 06/27 12:00 PM 3:45 PM 70 rttomo BPV 4 vacuum high at startup
Mon Jun 30 14:58:11 2008 Morphew 06/29 9:00 AM 2:30 PM f20cryo cryotomo BPV 5
Tue Jul 1 16:41:47 2008 Cindi 7/1 9:15 AM 4:45 PM standard rtimage EELS practice Rigged air regulator in TV camera
controller.
Wed Jul 2 16:26:59 2008 Cindi 7/2 2:00 PM 4:25 PM standard rtimage eels AUGH
Thu Jul 3 12:35:01 2008 morphew 07/02 9;00 AM 12:30 PM 70 rttomo PAPILLOMA VIRUS 0 i DUMPED THE VAC UPON ROD
INSERTION. vAC FAILED TO RECOVER ON
ITS OWN.
Thu Jul 3 17:08:13 2008 cindi 7/3 2:00 PM 5:00 PM standard rtimage eels, lipids
Tue Jul 8 08:57:36 2008 Cindi/Alexandra 07/07 9:00 AM 12:00 PM standard rtimage scope down turbo wasn't working correctly
Tue Jul 8 08:58:15 2008 Service/Chuck Moe 7/7 12:00 PM 2:00 PM standard serviceman worked on turbo pump diagnosed and got it working again
Tue Jul 8 08:59:09 2008 service/Chuck Moe 7/8 7:00 AM 9:00 AM standard serviceman drag pump replaced drag pump, still doesn't
work. controller card is bad.
Fri Jul 11 08:40:36 2008 Service/Chuck Moe 7/9 10:30 AM 12:30 PM standard serviceman install new turbo pump assembly received old version of turbo pump
(68). Installed and it was bad. It
was the last one available and all
of the rest are on backorder.
Fri Jul 11 08:41:06 2008 Scope Down 7/10 8:00 AM 6:00 PM standard serviceman scope down, bad turbo
Fri Jul 11 08:41:33 2008 Scope Down 7/11 8:00 AM 6:00 PM standard serviceman scope down, bad turbo
Mon Jul 14 14:58:04 2008 Service/Chuck Moe 7/14 10:30 AM 1:00 PM standard serviceman replace turbo pump turbo pump arrived. It was not
reconditioned, so it was not
working. Escalated to Head of
Logistics.
Tue Jul 15 09:36:06 2008 DNM 7/15 9:15 AM 9:30 AM standard development Checking out shuttering with both
idle states closed 0
Tue Jul 22 17:58:30 2008 Julia Cope 07/22 10:30 AM 5:45 PM 914 cryoimaging Kar3Vik1 mt's 0 Not vitrous vitrobot samples :( No water flow or current to
objective lens - Chuck fixed it but
beam was really wacky. He did some
fancy FEG realigments and it was ok
after that. Crashed vacuum twice,
but came back relatively quickly.
Stage makes a screeching noise when
moving long distances for lmm.
Wed Jul 23 16:34:16 2008 Cindi 07/23 9:40 AM 4:30 PM f30cryo cryoimaging cryo sections PTKGFP GIF beam was offset from regular
beam
Fri Jul 25 12:06:55 2008 morphew 7/25 11:00 AM 12:00 PM standard rtimage silicon oxide film check 0
Mon Jul 28 12:16:53 2008 bouchet-marquis 07/25 09:00 AM 17:30 PM 914 cryoimaging sections 0 crashed two times the vaccum at the
insertion of the rod.
crashed first rod at RT
then crashed when rod was cold.
Mon Jul 28 16:49:41 2008 morphew 07/28 9;00 AM 4:50 PM f20cryo cryotomo whole mount c127/bpv infect 5
Tue Jul 29 14:23:33 2008 Cindi 07/29 10:15 AM 2:20 PM f30cryo cryoimaging sxns stage will not move in Y, called
service.
Wed Jul 30 09:22:59 2008 Service R Spilman 12/30/2008 8:45 AM 9:20 AM standard serviceman Compustage call N11529 Y donator Noisey in negative
direction. Ordered new donator to
arrive Thursday 7/31/2008 5322-695-
15858 SEO 48851 Y donator Noisey in negative
direction. Ordered new donator to
arrive Thursday 7/31/2008 5322-695-
15858 SEO 48851
Wed Jul 30 18:36:34 2008 morphew 07/29 9 AM 6:30 PM 70 rttomo trypanosomes 8 2x1
Thu Jul 31 10:35:40 2008 Service rspilman 7/31/2008 9:00 AM 10:30 AM standard serviceman Compustage 0 Replaced donator for Y
translation. Adusted Y movements. Replaced donator for Y
translation. Adusted Y movements.
Fri Aug 1 10:50:18 2008 Cindi 07/31 2:30 PM 3:00 PM standard rtimage giardia neg stain
Fri Aug 1 10:50:36 2008 Cindi 07/31 6:00 PM 8:00 PM f30cryo cryoimaging sxns
Fri Aug 1 16:29:23 2008 morphew 08/01 9 AM 4:30 PM 70 rttomo trypanosomes 6
Mon Aug 4 16:47:00 2008 morphew 8/4 9 AM 4:45 PM f30cryo cryotomo c127 bpv infect 3
Mon Aug 11 17:05:38 2008 Eileen O'Toole 8/12 8:30 AM 5:00 PM 70 rttomo Odde metaphase yeast 7 The compustage had a siezure when I
was adjusting eucentricity this
morning. Called Chuck and he walked
me through reseting.
Tue Aug 12 16:51:48 2008 Eileen 8/12 8:30 AM 5:00 PM 70 rttomo Odde metaphase yeast 8
Wed Aug 13 16:42:42 2008 morphew 08/13 9 AM 4:35 PM f20cryo cryotomo bpv isolated 1 Stage started going hay-wire at
touch of translation (not tilted).
Disabled stage, removed holder,
tried to re-enable, wouldn't go.
Tried 4 times. Abandoned scope,
called and left message for Chuck,
waited, started cryo-cycle, tried
once again, this time it did re-
enable. Left scope without holder
in stage (since cryocycle was
going). Called Chuck and left
another message.
Thu Aug 14 16:43:54 2008 morphew 08/14 9 AM 4:45 PM 70 rttomo bpv isolated cryo, trypanosomes
plastic 5
Mon Aug 18 16:13:27 2008 morphew 08/18 8:45 AM 4:15 PM 70 rttomo malaria--Tim Spurk, Univ of
Melbourne 4
Tue Aug 19 17:07:08 2008 morphew 8/19/08 2 PM 5:15 PM 70 rttomo melanoma 2
Tue Aug 19 17:08:00 2008 Jose Maria 8/19 9 AM 2 PM 70 rttomo plant nuclei 3
Thu Aug 21 16:07:50 2008 Cindi 8/21 10:00 AM 4:00 PM f30cryo cryotomo Eg5 Microtubules 4 Stage tried to go to -1288 in Y
during LMM. Took holder out, reset
goniometer, wouldn't reset. David
manually moved the stage about 1mm,
then it reset. C2 aperture was at
150um instead of 100um
Fri Aug 22 17:16:01 2008 Jose Maria 08/22 10 AM 5:30 PM standard rttomo Tomography of plant nuclear
structures 8 Hi Cindi,
Besides the problem mentioned
below, I just wanted to thank you
for all of your help during these
days.
I will leave next week, and I hope
to find you around on tuesday to
say you goodbye. At low mag, the beam was doing
weird things, like losing their
circular shape or projecting like
shadows. However, at x20000,
everything was perfect. It did not
bother me at all.
Mon Aug 25 12:24:59 2008 Chuck/Service 08/25 8:00 AM 9:30 AM f30cryo serviceman check stage worked on F30 stage problem and tightened the
y-drive. tested movement with cryo holder.
going to each y extreme and tilting with no y-
axis error.
Mon Aug 25 16:07:47 2008 morphew 8/25 11:00 AM 4:00 PM 70 rttomo melanoma cells correlative 3
Tue Aug 26 12:16:45 2008 cindi 08/26 9:30 AM 10:30 AM f30cryo cryotomo eg5 MTs stage got stuck. Chuck had to come
Tue Aug 26 12:21:39 2008 Service/Chuck 08/26 11:00 AM 12:30 PM f30cryo cryotomo eg5 MTs stage got stuck in Y axis . Rebuilt
Y axis motor assy and tested the
movement
Thu Aug 28 15:51:01 2008 morphew 08/28 9 AM 3:50 PM 70 rttomo freeze sub tests 2
Fri Aug 29 14:28:02 2008 morphew 8/29 9 AM 2:30 PM f30cryo cryotomo BPV/DTT treat 2
Tue Sep 2 16:04:15 2008 Eileen 9/2 9 AM 4 PM tiltrotate rttomo chlamy mitotic 2 first session with returned tilt
rotate rod. Worked well!
Wed Sep 3 15:52:11 2008 cindi 9/3 9:30 AM 4:00 PM f30cryo cryotomo eg5 MTs 2 Also looked at plastic sections in
cryo-holder.
Thu Sep 4 16:26:20 2008 morphew 9/4 9 AM 4:24 PM tiltrotate rttomo tryps 4
Fri Sep 5 15:50:10 2008 morphew 9/5 2 PM 3 PM standard rtimage silicon antibody binding
Fri Sep 5 15:51:25 2008 cindi 9/5 3:15 PM 3:50 PM standard development calibrations for ctf noise
Mon Sep 8 15:23:38 2008 Eileen 9/8 8 AM 3:30 PM 70 rttomo U2OS mitotics 4 Gave 'stage end of range' two times
when taking the rod out of the
scope although it had been centered
in x/y. No problem, though, just a
weird message.
Tue Sep 9 16:39:29 2008 cindi 9/9 9:30 AM 4:45 PM 914 cryoimaging S-layers, BPV infected cells Tried diffraction to see when cells
de-vitrify. Also used F30 cryo
holder.
Fri Sep 12 08:35:43 2008 cedric 9/11 10 AM 12 PM 914 cryotomo vitreous sections 0 2 hard vac crashes. quit for the day
Fri Sep 12 08:36:53 2008 eileen 9/11 1 PM 3:15 PM tiltrotate rttomo U2Os overlap zone 1 1x2 montage
Fri Sep 12 15:12:00 2008 eileen 9/12 8:15 AM 3:15 PM tiltrotate rttomo U2OS midzone 5 1x2 montages
Tue Sep 16 17:39:05 2008 eileen 9/16 9 AM 6 PM tiltrotate rttomo U2OS supermontage, section 1 4 Four,3x3 montages for 2x2 super
Thu Sep 18 12:54:45 2008 cindi 09/18 9:30 AM 1:00 PM f30cryo cryoimaging PTK sections from cryo-LM Nothing to see here.
Mon Sep 22 11:42:04 2008 cindi 9/22 10:00 AM 11:30 AM 914 cryoimaging giardia grid bad crashed vacuum inserting rod
Mon Sep 22 16:07:32 2008 cindi 9/22 11:30 AM 4:15 PM f30cryo cryotomo giardia 4 sample looked good
Wed Sep 24 13:39:54 2008 cindi 9/24 12:00 PM 1:45 PM 914 cryoimaging slayers first grid had cauliflower ice and
the rest were too thick lost ability to select holder when
inserting rod
Wed Sep 24 17:19:08 2008 Matt West 9/23 7:30 AM 7:30 PM standard rttomo Yeast ER 14 This session was for Gia Voeltz lab.
Scope worked great!
Thu Sep 25 14:54:00 2008 morphew 9/25/08 8:45 AM 3:00 PM f30cryo cryoimaging melanoma whole mount 0
Fri Sep 26 15:57:15 2008 cindi 9/26 9:30 AM 3:30 PM f30cryo cryotomo s-layers 2 wouldn't let me pick the holder,
but still worked
Mon Sep 29 16:50:08 2008 morphew 9-29 9 AM 4:45 PM tiltrotate rttomo chloroplast 8
Tue Sep 30 15:27:12 2008 morphew 9-30 9 AM 3:30 PM f30cryo cryotomo bpv 4
Thu Oct 2 16:53:35 2008 morphew 10-2 10 AM 5 PM tiltrotate rttomo melanoma 4
Fri Oct 3 15:48:29 2008 morphew 10-3 1 PM 4 PM tiltrotate rttomo bpv
Mon Oct 6 18:01:25 2008 morphew 10/6 8:30 AM 6 PM f30cryo cryotomo bpv 6
Tue Oct 7 13:21:24 2008 Eileen 10/7/08 8:50 AM 1:20 PM tiltrotate rttomo isolated thylakoids 4
Thu Oct 9 12:30:24 2008 eileen 10/9/08 8:45 AM 12:30 PM 70 rttomo isolated thylakoids 4 extraction voltage was below 4250
so beam was dimmer than usual. I
didn't notice until after the
alignments.
Fri Oct 10 16:54:37 2008 morphew 10/10/08 9 AM 5 PM tiltrotate rttomo bpv 2-2x2
Sat Oct 11 01:34:05 2008 Johanna 10/10 8 PM 01:30 AM fishione rttomo Trypanosomes 4
Sun Oct 12 04:03:51 2008 Johanna 10/11 5:00 PM 04:00 AM fishione rttomo trypanosomes 10 Montages
Mon Oct 13 04:10:32 2008 Johanna 12/10 3:00 PM 4:00 AM fishione rttomo trypanosomes 9 montages
Mon Oct 13 17:00:06 2008 morphew 10/13 3 AM 5 PM tiltrotate rttomo thylakoid 2
Tue Oct 14 16:42:47 2008 morphew 10-14 8:45 AM 4:45 PM f30cryo cryotomo bpv-infected whole mount 7
Wed Oct 15 23:47:19 2008 Johanna 10/15 7:00 PM 00:00 PM fishione rttomo trypanosomes 7 montages
Thu Oct 16 08:22:03 2008 cindi 10/15 10:30 PM 5:00 PM f30cryo cryoimaging NRK cryo-sections The correlation from LM to EM
worked quite well.
Thu Oct 16 21:10:50 2008 Johanna 10/16 3:00 PM 9:00 PM fishione rttomo trypanosome 8
Fri Oct 17 16:33:15 2008 Janet Meehl 10/17 12:15 PM 4:45 PM tiltrotate rttomo Tetrahymena exchange junction 3
Mon Oct 20 11:03:21 2008 Johanna 10/18 3:00 PM 11:00 PM fishione rttomo trypanosome 6
Mon Oct 20 20:47:42 2008 cindi 10/20 10:30 AM 8:45 PM f30cryo cryotomo ice and cryo-sections 3 5th grid's the charm :)
Tue Oct 21 18:16:33 2008 eileen 10/21/08 9 AM 6:30 PM tiltrotate development auto-acquire with SerialEM 2 3x3 David and I worked on setting up
supermontage auto-acquiring
functions with Macros, Navigator
and Serialem. Very cool! 2 3x3
montages from a supermontage map
Wed Oct 22 01:45:57 2008 JOhanna 10/21 06:30 PM 02:00 AM fishione rttomo leishmania 6
Wed Oct 22 14:44:01 2008 cindi 10/22 9:15 AM 2:45 PM f30cryo cryotomo giardia 2 test of correlative lm/em
Thu Oct 23 13:27:33 2008 Janet Meehl/Eric Cole 10/23 8:00 AM 1:30 PM tiltrotate rttomo nuclear exchange junction - Tet 6 WOW!
Fri Oct 24 18:16:56 2008 Johanna 10/24 9:00 AM 6:15 PM fishione rttomo Leishmania 14
Tue Oct 28 16:40:04 2008 cindi 10/28 10:15 AM 4:45 PM f30cryo cryotomo giardia + NC1Kinesin 3 4th grid's the charm. Column Vacuum was at 20 when I
logged in. O-ring was a bit dirty.
Wed Oct 29 15:46:20 2008 cindi 10/29 9:30 AM 3:45 PM f30cryo cryoimaging cryo-sxns and BPV infected cells sxns lost and cells not vitreous :(
Wed Nov 5 14:18:57 2008 Janet Meehl/Eric Cole 11/5 10:50 AM 2:15 PM tiltrotate rttomo Tetrahymena nuclear exchange
junction 0
Wed Nov 12 15:29:39 2008 eileen 11/12/08 8:30 AM 3:45 PM tiltrotate rttomo Andrea R. synapses 8
Thu Nov 13 14:39:11 2008 eileen 11/13 8:30 AM 2:30 PM tiltrotate rttomo Andrea's 3minute synapse 4 1x2
Sat Nov 15 22:23:58 2008 Johanna 11/15 1:30 PM 11:00 PM fishione rttomo tryps 4 montages
Mon Nov 17 00:39:35 2008 Johanna 11/17 2:00 PM 00:30 AM fishione rttomo tryps 7 montages
Tue Nov 18 14:58:11 2008 Ron 11/17 1:30 PM 5:00 PM standard serviceman Replace objective aperture lever replace objective aperture lever
Tue Nov 18 15:00:19 2008 Ron 11/18 11:30 PM 3:00 PM standard serviceman preventative maintenance replace hoses, check chiller,
change pump oil, replace water
filter, checked image, electronic
vacuum test
Tue Nov 18 17:10:31 2008 eileen 11/18 3:30 PM 5:15 PM 70 rtimage chlamy mitotics 0 The +/- rod looked slightly bent.
Wed Nov 19 16:47:43 2008 morphew 11/19 9 AM 5 PM tiltrotate rttomo tryps 6
Thu Nov 20 15:46:12 2008 eileen 11/20 8:25 AM 3:45 PM tiltrotate rttomo yeast and chlamy 2 1x2
Fri Nov 21 15:38:10 2008 eileen 11/21 8:30 AM 3:45 PM tiltrotate rttomo chlamy, yeast 3
Tue Nov 25 18:30:53 2008 DNM 11/25 2:15 PM 6:30 PM standard development cooker and autocenter beam
Wed Nov 26 13:26:41 2008 eileen 11/26 8:30 AM 1:30 PM tiltrotate rttomo yeast spindles 6
Wed Nov 26 17:47:14 2008 DNM 11/26 1:30 PM 5:50 PM standard development autocenter, cook, special film
command The HT went off and would not
restart when I started using the
adaexp server in "show" mode. I
had to reboot. Then I started
using it in SerialEM and the HT did
the same thing. Another reboot,
and things went fine for 2 hours,
so it really was the adaexp
server. I will get a proper
version for the scope from Max
Otten.
Tue Dec 2 16:28:58 2008 eileen 12/2 8:30 AM 4:30 PM tiltrotate rttomo chlamy 5 1x2
Wed Dec 3 15:09:08 2008 service 12/1 8:00 AM 5:00 PM standard serviceman F30 computer off needs new power supply. put in
temporary power supply
Wed Dec 3 15:09:48 2008 cindi 12/3 10:00 AM 3:00 PM tiltrotate rtimage giardia looked like crap
Thu Dec 4 15:26:23 2008 eileen 12/4 11:00 AM 3:30 PM tiltrotate rttomo chlamy 2
Fri Dec 5 15:00:36 2008 david 12/5 10:30 AM 11:00 AM standard serviceman replace power supply replace power supply on scope
computer
Mon Dec 8 15:49:29 2008 morphew 12-8 1 PM 4 PM fishione rttomo yeast 2
Wed Dec 10 17:10:59 2008 eileen 12/20 10:30 AM 5:00 PM tiltrotate rttomo Pietro De Camilli neurons 4
Thu Dec 11 12:58:25 2008 eileen 12/11 8:45 AM 1:00 PM tiltrotate rttomo Chlamy mitotics 4 1X2
Fri Dec 12 16:41:09 2008 Chuck Robertson 12/12 8:30am AM 4:30 PM fishione rttomo Methanosarcina barkerei 4 Ok
Tue Dec 16 15:45:24 2008 eileen 12/16 9:30 AM 3:45 PM tiltrotate rttomo chlamy mitotics 4 1x2 rod was not put back in the
microscope from the day before. It
took a while for the vac to come
down.
Wed Dec 17 15:34:46 2008 morphew 12-16 9 AM 4 PM tiltrotate rttomo bpv, baas neurons 3
Thu Dec 18 15:13:28 2008 eileen 12/17/08 8:45 AM 3:15 PM tiltrotate rttomo DeCamilli serials 3 2x2
Fri Dec 19 16:47:42 2008 Janet Meehl/Winey 12/19 9:10 AM 4:45 PM tiltrotate rttomo WT tetrahymena duplicatin basal
bodies 11
Mon Dec 22 13:24:02 2008 Janet Meehl/Winey 12/22 10:15 AM 1:30 PM tiltrotate rttomo WTtet duplicatin basal bodies 4
Mon Dec 22 15:49:13 2008 DNM 12/22 2:30 PM 3:45 PM standard serviceman trying to get the GIF going again Got beam on cameras but it won't
tune
Wed Dec 31 02:44:47 2008 Chuck Robertson 12/30 8:30 AM 3:00 AM fishione rttomo Methanosarcina barkeri 20 Previous user (Christine) appears
not to have made log book entry...
did not have context for problem
noted below.
F30 was very stable today, but the
beam is marginal for my samples.
Today the beam was 8.26 nA st spot
size = 2. On arrival found the extraction
voltage at 3800... no indication in
the log of problem or not by
previous user. Also, turbo pump
was off on arrival (I thought it
was supposed to run all of the time
now after it cryocycled?)
Brought the EV back to 4250...
everything appeared not to fail.
Mon Jan 5 14:49:32 2009 eileen 1/5 8:40 AM 2:50 PM tiltrotate rttomo Davis yeast 6
Mon Jan 5 17:26:39 2009 DNM 1/5 3:00 PM 5:30 PM standard serviceman diagnosing GIF problems with VNC Scott thinks the replacement board
is bad or there is something bad
downstream.
Tue Jan 6 18:38:21 2009 cindi 01/06 8:15 AM 11:45 AM 914 cryotomo giardia no samples on grids
Tue Jan 6 18:38:43 2009 cindi 01/06 2:00 PM 6:40 PM 70 rttomo giardia samples look OK
Tue Jan 6 18:38:52 2009 cindi 01/06 2:00 PM 6:40 PM 70 rttomo giardia 3 samples look OK
Thu Jan 8 17:18:24 2009 morphew 1/9 9 AM 5 PM f30cryo cryotomo vessicles 3
Sat Jan 10 16:36:53 2009 cindi/moises 01/10 10:00 AM 4:30 PM 914 cryotomo giardia cytoskeletons no good samples :(
Mon Jan 12 16:19:50 2009 morphew 1-12 9 AM 4 PM f30cryo cryotomo protein complex-stowell 1
Tue Jan 13 15:59:36 2009 cindi/moises 01/13 1:00 PM 4:00 PM 914 cryotomo giardia crappy grids
Tue Jan 13 19:21:23 2009 eileen 1/13 4:00 PM 7:30 PM tiltrotate rttomo Trisha Davis yeast 6
Wed Jan 14 16:52:56 2009 Julia 1/14 12:45 PM 4:50 PM f20cryo cryoimaging Kar3Vik1 mts, apyrase
Thu Jan 15 21:40:48 2009 cindi/moises 01/15 8:00 AM 9:45 PM 914 cryotomo Giardia attached cytoskeletons 10 finally!!
Fri Jan 16 17:21:45 2009 morphew 1/16 9 AM 5:30 PM tiltrotate rttomo melanoma 4
Mon Jan 19 16:53:00 2009 cindi 1/19 2:30 PM 4:45 PM tiltrotate development distortion correction images
Tue Jan 20 17:59:18 2009 eileen 1/20 8:30 AM 6:00 PM tiltrotate rttomo Trisha Davis yeast 12
Wed Jan 21 17:11:27 2009 morphew 1/21 8:00 AM 5:00 PM tiltrotate rttomo aditi neurons 6, 2x2
Thu Jan 22 18:02:50 2009 Julia 1/21 9:00 AM 6:00 PM f20cryo cryoimaging Kar3Vik1 mt Apyrase 0 All good
Fri Jan 23 18:01:25 2009 Janet Meehl/Winey 1/23 10:15 AM 6:00 PM tiltrotate rttomo WT tetrahymena basal bodies 10
Mon Jan 26 15:41:31 2009 eileen 1/26/09 8:40 AM 3:45 PM tiltrotate rttomo chlamy mitotics 9
Tue Jan 27 15:18:25 2009 morphew 1-27 8:45 AM 3:15 PM tiltrotate rttomo aditi, neurons 4
Tue Jan 27 18:11:43 2009 eileen 1/27 3:15 PM 6:30 PM tiltrotate rttomo Chlamy poles 4
Wed Jan 28 17:53:29 2009 Janet Meehl/Winey 1/28 1:30 PM 6:00 PM tiltrotate rttomo WT tetrahymena basal bodies 4
Thu Jan 29 19:35:34 2009 Matthew West 01/28 9:00 AM 7:45 PM tiltrotate rttomo WT ER 16 Voeltz lab
Fri Jan 30 15:29:11 2009 eileen 1/30 11 AM 3:30 PM tiltrotate rttomo chlamy mitotics 4
Mon Feb 2 14:36:56 2009 eileen 2/2 8:45 AM 2:30 PM tiltrotate rttomo chlamy interphase 6
Tue Feb 3 19:19:13 2009 cedric 02/03 09:00 AM 20:00 PM 914 cryotomo section 4
Wed Feb 4 16:57:43 2009 morphew 2-4 9 AM 5 PM f30cryo cryotomo c127 w/bpv 6
Thu Feb 5 16:09:43 2009 eileen 2/5 9 AM 4:15 PM tiltrotate rttomo RNAi gamma 5
Mon Feb 9 15:35:47 2009 eileen 2/9 8:30 AM 3:30 PM 70 rttomo RNAi gamma 5
Tue Feb 10 16:05:46 2009 morphew 2/10 8:45 AM 4:15 PM tiltrotate rttomo aditi neurons 4-1x3
Wed Feb 11 19:15:57 2009 cedric 02/11 09:00 AM 8:00 PM 914 cryotomo sections 4
Thu Feb 12 17:32:18 2009 Julia 2/12 9:00 AM 5:30 PM f30cryo cryoimaging kar3vik1 mts apyrase All good
Mon Feb 16 16:05:40 2009 morphew 2/16 9 AM 4 PM f30cryo cryotomo c127 virus 3
Tue Feb 17 16:00:55 2009 cindi 12/17 10:30 AM 4:00 PM 914 cryoimaging giardia no good samples
Wed Feb 18 16:55:26 2009 Chris Arthur 02/18 10:00 AM 5:00 PM f30cryo cryotomo Mouse synaptosomes 0 Crashed vacuum 3 times and figured
it was not meant to be today. Will
try again in the morning.
Thu Feb 19 15:17:51 2009 spilman 2/19 10:00 AM 3:00 PM standard serviceman test feg hardware falure after HT arc.
Conditioned HT to 305KV
checked rapid Dave open 1194 port
checked operation with TSG
Thu Feb 19 19:41:02 2009 Chris Arthur 02/19 9:00 AM 8:00 PM 914 cryotomo Mouse synaptosomes 3 FEG arcing problem but Ron said it
was okay
Fri Feb 20 18:07:18 2009 cedric 02/20 13:00 PM 18:00 PM 914 cryoimaging sections 0 beam blinks when playing with
intensity
beam vibrates when concentrated
Sun Feb 22 16:29:17 2009 cindi 2/22 9:00 AM 4:30 PM 914 cryotomo giardia cytoskeletons 8
Sun Feb 22 17:39:30 2009 cindi 02/22 4:30 PM 5:30 PM 70 rtimage giardia check-out
Mon Feb 23 16:05:20 2009 morphew 2-23 8:30 AM 4 PM f30cryo cryotomo 3t3's plus motors 5
Tue Feb 24 18:36:22 2009 Chris Arthur 02/24 9:00 AM 6:45 PM 914 cryotomo Mouse synaptosomes 4
Thu Feb 26 17:02:45 2009 Julia 2/26 9:00 AM 5:00 PM f20cryo cryoimaging Eg5A3 mts scope good, samples bad
Fri Feb 27 15:50:05 2009 morphew 2-27 9 AM 4 PM f30cryo cryotomo membrane channels 5
Sun Mar 1 16:33:15 2009 cindi 3/1 9:00 AM 1:30 PM 914 cryoimaging bad giardia grids
Sun Mar 1 16:33:35 2009 cindi 3/1 1:30 PM 4:30 PM tiltrotate rttomo giardia 2
Tue Mar 3 09:36:08 2009 Chris Ackerson 03/02 8:55 AM 4:00 PM standard rtimage EELS attempts bad columns in CCD?- David M was
working on this when I left
Tue Mar 3 17:41:03 2009 morphew 3-3 8:15 AM 5:45 PM tiltrotate rttomo aditi neurons 6 2x1
Wed Mar 4 16:57:36 2009 morphew 3-4 8:15 AM 5 PM f30cryo cryotomo 3t3 w/eg5 7
Thu Mar 5 11:08:07 2009 DNM 3/3 4:00 PM 5:00 PM standard serviceman adjusting defect table in DM for GIF 0 Added some regions to GIF defect
table. Aslo determined that the
bottom/right coordinate in the
defect table has to be 1 past the
desired pixel
Thu Mar 5 16:40:20 2009 cindi 3/5 10:30 AM 4:45 PM 914 cryotomo giardia attached 4 for some reason, my gif was -130
defocus from the upper camera. just
changed focus with screen up to 0
and then everything was fine.
Fri Mar 6 17:10:43 2009 cedric 03/06 10:00 AM 17:30 PM f20cryo cryotomo sections 3
Mon Mar 9 15:26:20 2009 eileen 3/9 8:30 AM 3:30 PM 70 rttomo Gamma RNAi 6
Tue Mar 10 17:15:47 2009 morphew 3-10 8:45 AM 5:15 PM tiltrotate rttomo eric melamoma
Wed Mar 11 19:45:00 2009 cindi 3/11 9:00 AM 7:45 PM 914 cryotomo giardia cytoskeletons 6 woo hoo!!!!!!
Thu Mar 12 18:30:16 2009 cedric-sarah 03/12 9:30 AM 6:30 PM f20cryo cryoimaging tubes-tau1 0
Fri Mar 13 18:19:35 2009 Matthew West 03/13 7:00 AM 6:30 PM tiltrotate rttomo ER Mutant / Thick Section 18 Voeltz lab
Mon Mar 16 14:50:16 2009 morphew 3-16 9 AM 3:45 PM f30cryo cryotomo g127 plus BPV 0 gif doesn't seem to always
continue tuning after needing to
reset the beam intensity
Tue Mar 17 17:24:47 2009 eileen 3/17 8:30 AM 5:30 PM 70 rttomo Gamma RNAi 10
Wed Mar 18 11:26:56 2009 cindi 3/18 9:00 AM 11:30 AM 914 cryotomo giardia cytoskeletons cryo sucks.
Mon Mar 23 14:53:35 2009 eileen 3/23 8:30 AM 3:00 PM 70 rttomo Odde yeast prometa and maps 6
Tue Mar 24 20:18:00 2009 Chris Arthur 03/24 9:00 AM 8:00 PM 914 cryotomo Synaptosomes 9 Thanks Cindi
Wed Mar 25 17:19:36 2009 Chris Arthur 03/25 9:00 AM 5:30 PM 914 cryotomo Synaptosomes 6
Wed Apr 1 17:59:57 2009 Johanna/Kristin 4/1 9:30 AM 6 PM fishione rttomo Trypanosome flagella connector 2
Thu Apr 2 17:17:06 2009 Matthew West 04/02 11:00 AM 5:15 PM tiltrotate rttomo Plastic Yeast 10 Voeltz lab crashed the vacuum putting the
standard rod in at the last step
before cryo-cycle
Fri Apr 3 14:59:28 2009 cedric 04/03 09:00 AM 15:00 PM f20cryo cryoimaging section-plunge frozen cells 0
Sun Apr 5 16:36:19 2009 cindi 04/05 9:00 AM 4:30 PM standard rtimage giardia thin sxns
Mon Apr 6 13:47:27 2009 morphew 4-06 9 AM 2 PM f30cryo cryotomo c127-bpv 3
Mon Apr 6 21:48:18 2009 Johanna/Kristin 04/06 2:00 PM 10:00 PM fishione rttomo Trypanosomes 3
Tue Apr 7 17:55:22 2009 eileen 4/7/09 9:15 AM 6:00 PM 70 rttomo WT centrosomes 8
Wed Apr 8 16:12:46 2009 morphew 4-8 8:45 AM 4:45 PM f30cryo cryotomo bpv-c127 1
Thu Apr 9 12:58:26 2009 eileen 4/9/09 9:30 AM 1:00 PM 70 rttomo WT centrosome series 6
Thu Apr 9 21:12:06 2009 Johanna/Kristin 4/9 1:00 PM 9:10 PM fishione rttomo trypanosome 4
Fri Apr 10 17:26:07 2009 cedric 04/10 09:00 AM 18:00 PM 914 cryoimaging sections 0
Mon Apr 13 18:52:08 2009 Matthew West 04/13 8:30 AM 7:00 PM tiltrotate rttomo ER triple mutant 16 Voeltz lab tomography
Tue Apr 14 17:40:54 2009 eileen 4/14 1:15 PM 5:45 PM 70 rttomo endophilin DKO (De Camilli) 4
Wed Apr 15 19:55:17 2009 Johanna/Kristin 04/15 1:00 PM 8:15 PM fishione rttomo chlamydomonas 4
Wed Apr 15 19:56:07 2009 Johanna/Kristin 04/15 1:00 PM 8:15 PM fishione rttomo chlamydomonas 4 Stage lost communication with
multifunction knobs and trackball.
Thu Apr 16 14:01:51 2009 morphew 04/16 8:45 AM 2 PM tiltrotate rttomo bpv_c127 2-2x1
Fri Apr 17 11:14:59 2009 eileen 4/17 8:15 AM 11:15 AM 70 rttomo endophilin TKO (De Camilli) 4
Mon Apr 20 20:19:59 2009 Kristin/Johanna 04/20 12:30 PM 8:30 PM fishione rttomo trypanosomes 6
Tue Apr 21 17:39:53 2009 eileen 4/21 8:45 AM 5:45 PM 70 rttomo gamma RNAi late mitotic; endophilin
wt control (De Camilli) 10
Wed Apr 22 15:59:28 2009 morphew 4-23 9 AM 4 PM f30cryo cryotomo bpv 0
Wed Apr 22 20:52:34 2009 Johanna 04/22 4:00 PM 9:00 PM fishione rttomo trypanosomes 4
Thu Apr 23 15:50:56 2009 cedric 04/23 9:00 AM 17:00 PM f30cryo cryoimaging metallothionein 0
Tue Apr 28 15:32:57 2009 morphew 4-28 10:30 AM 3:30 PM tiltrotate rttomo bpv 4
Thu Apr 30 16:34:04 2009 cedric 04/30 9:00 AM 17:00 PM f30cryo cryoimaging tau-mth2-gold 0
Fri May 1 19:35:31 2009 Johanna 5/1 10:00 AM 7:30 PM fishione rttomo trypanosomes 7
Sat May 2 18:25:54 2009 Johanna 5/2 1:30 PM 6:30 PM fishione rttomo chlamydomonas 6
Sun May 3 19:20:21 2009 Johanna 5/3 12:30 PM 7:15 PM fishione rttomo chlamydomonas/trypsanosoma 4 I had to insert the rod several
times, cleaning it before and
greasing the o-ring, since the
vaccum stayed up. Still, the vaccum
crashed when rod inserted the
third/fourth time. After this, no
more problems though.
Mon May 4 21:07:24 2009 Johanna 5/4 1:00 PM 9:00 PM fishione rttomo trypanosome 10
Tue May 5 20:29:44 2009 johanna 5/5 2:00 PM 8:30 PM fishione rttomo trypanosomes 6
Wed May 6 18:30:31 2009 Julia 5/6 1:30 PM 6:27 PM f30cryo cryoimaging Eg5A3 mts All good
Thu May 7 13:25:33 2009 morphew 05-07 7:45 AM 1:22 PM f30cryo cryotomo mechanosensitive channels 4
Thu May 7 15:27:00 2009 Cindi 05/07 2:15 PM 3:30 PM standard rtimage giardia neg stain samples sucked
Fri May 8 16:28:21 2009 Ron Spilman 5/08 1:00 PM 5:00 PM standard serviceman Gold 2.35A resolution 0 PM, Made electronic and vacuum
checks, backup hard drive, checked
resolution, drain compressor,check
hoses and fittings.
Mon May 11 16:09:28 2009 Johanna 05/11 10:00 AM 4:00 PM fishione rttomo tryps/chlamy 0 The vacuum was on 20 when I arrived
and did not go down even after an
hour with liquid nitrogen.
I noticed that the compustage had
not been reset. Coincidence or not?
Tue May 12 17:01:44 2009 eileen 5/12 8:30 AM 5:00 PM 70 rttomo DeCamilli Endophilin DKO and
Dynamin TKO neurons 9
Wed May 13 15:43:53 2009 Kristin/Cindi 5/13 1:00 PM 3:45 PM tiltrotate rttomo septin delete pombe cells 1 T/R holder does NOT rotate. The
motor makes no noise. Getting an
RMA to send to Gatan for repair.
Thu May 14 17:36:22 2009 Janet Meehl/Odde 5/14/09 9:10 AM 5:45 PM 70 rttomo yeast cik1del spindles 9
Fri May 15 16:37:07 2009 morphew 5-15 9 AM 4:30 PM f30cryo cryotomo mechanosensitive channel 4
Mon May 18 18:25:48 2009 Kristin 5/18 12:30 PM 6:20 PM fishione rttomo trypanosomes 4 I selected 'keep intensity below
current value' but when I started
the tilt series, the beam didn't
spread so the image became
overexposed... Had to perform tilt
series without that 'keep
intensity' option selected.
Tue May 19 19:51:32 2009 Matthew West 5/21 8:30 AM 8:00 PM standard rttomo Yeast MVBs / ER 14 6 TS were for Odorizzi (5 hrs) and
8 TS were for Voeltz (6.5 hrs)
Wed May 20 17:11:18 2009 Janet Meehl/Odde 5/20/09 9:20 AM 5:10 PM 70 rttomo yeast cik1delete spindles 12
Fri May 22 14:06:34 2009 cedric 05/22 11:00 AM 2:00 PM f30cryo cryoimaging unknown
Wed May 27 17:35:21 2009 Kristin 5/27 11:30 AM 5:30 PM fishione rttomo yeast 2
Mon Jun 1 11:13:49 2009 eileen 5/28 12:30 PM 4:30 PM 70 rttomo Chlamy 400nm wt 1
Mon Jun 1 11:15:19 2009 eileen 5/29 8:30 AM 5:30 PM 70 rttomo Chlamy 400nm wt 6 E: drive died during session. David
tested the drive and is returning
it.
Mon Jun 1 15:53:36 2009 eileen 6/1 1:15 PM 4:00 PM 70 rtimage mapped out tbg1(RNAi) poles
Wed Jun 3 09:28:33 2009 eileen 6/2/09 9:15 AM 5:15 PM 70 rttomo tbg1(RNAi) 7 1x2; 1 2x2
Wed Jun 3 12:31:45 2009 eileen 6/3/09 9:15 AM 12:30 PM 70 rttomo tbg1(RNAi) 3 1x2
Wed Jun 3 17:10:51 2009 Janet Meehl/Winey 6/3/09 12:30 PM 5:20 PM 70 rttomo WT tet duplicating basal bodies 7
Thu Jun 4 10:59:30 2009 eileen 6/4/09 8:30 AM 11:00 AM 70 rttomo tbg1(RNAi) midzone 1 2x2
Thu Jun 4 18:21:43 2009 Kristin 6/4 2:00 PM 6:30 PM fishione rttomo pombe 2
Mon Jun 8 16:12:16 2009 morphew 6/8/09 10 AM 4 PM 70 rttomo low temp freeze sub 4 beam turned-off once, at random
Tue Jun 9 14:22:54 2009 cindi/derek 06/09 8:00 AM 2:15 PM f30cryo cryotomo telomeric complexes 2
Thu Jun 11 08:43:41 2009 DNM 6/10 4:30 PM 6:00 PM 70 training preparing for workshop demo
Thu Jun 11 08:44:26 2009 DNM 6/11 6:15 AM 8:45 AM 70 training demo to workshop in England
Thu Jun 11 16:30:21 2009 Johanna 06/11 10:00 AM 5:00 PM fishione rttomo S. pombe 2
Fri Jun 12 16:29:45 2009 eileen 6/12/09 8:45 AM 4:30 PM fishione rttomo tbg1(RNAi) 6 ts + 2 1x2 imaged 1x2 montages with +/- 70 rod
then used fishione for other 6 ts
Tue Jun 16 21:21:29 2009 Matthew West 06/16 7:30 AM 9:30 PM fishione rttomo ER Mutant, Voeltz lab 18 Voeltz lab tomography--Scope is
working great! +/- 60 standard rod is slightly bent
Wed Jun 17 16:48:10 2009 morphew 6/17 9 AM 5 PM 70 rttomo low temp freeze sub 4
Thu Jun 18 16:04:14 2009 Kristin 6/18 1:50 PM 4:00 PM fishione rttomo trypanosomes 0
Fri Jun 19 15:49:01 2009 cindi 06/19 9:15 AM 3:45 PM standard rtimage giardia/pombe samples sucked! Found C2 aperture at 150um instead
of 100um. put back to 100um
Thu Jun 25 16:49:03 2009 cindi/david 06/25 9:30 AM 4:30 PM standard serviceman alignments redid EFTEM alignments as well as
all scope alignments. Use New062509
as the alignment file
Fri Jun 26 14:57:07 2009 Cynthia 06/24 10:00 AM 4:30 PM f20cryo cryoimaging ifs Gif needed tuning - Cindi did new
alignments on 6/25
Sat Jun 27 15:44:02 2009 cynthia 06/27 10:00 AM 3:30 PM f30cryo cryoimaging ifs tried to use gif! Hi Cindi, I was not success full
using the gif today. I am not sure
what the problem was but after
tuning the gif I couldn't find a
beam. Tried to redo alignments and
repeat gif tuning but then DM
didn't read beam even though I
could see it... message intensity
too low! Screen was up.
Tue Jun 30 22:20:04 2009 Matt West / Nesia Zurek 06/30 8:45 AM 10:30 PM fishione rttomo ER of Sc at bud sites: WT, ER
mutant 18 Voeltz lab session
Wed Jul 1 14:19:13 2009 eileen 7/1/09 8:30 AM 2:15 PM fishione rttomo tbg1(RNAi) PNM 4
Thu Jul 2 16:13:02 2009 morphew 7/2 10 AM 4 PM fishione rttomo low temp freeze sub 4
Wed Jul 8 16:52:57 2009 DNM 07/08 2:30 PM 5:00 PM 70 serviceman Fixing low mag alignments
Thu Jul 9 11:25:08 2009 cindi 07/09 9:15 AM 11:20 AM standard serviceman realignments
Thu Jul 9 18:59:32 2009 Kristin/Johanna 07/09 11:30 AM 7:30 PM fishione rttomo anterior end trypansoma 4
Fri Jul 10 15:52:58 2009 Johanna 07/10 10:00 AM 4:00 PM fishione rtimage trypanosome telophase 2 montages
Mon Jul 13 16:44:33 2009 Johanna 07/13 9:00 AM 4:30 PM fishione rtimage trypanosome telophase 4 montages
Tue Jul 14 19:51:04 2009 Matthew West 07/14 9:30 AM 8:00 PM fishione rttomo MVBs of Yeast, Bro1 Domain
Overexpression 16 Odorizzi lab
Wed Jul 15 16:41:46 2009 cindi/chieze 04/15 9:00 AM 4:45 PM 70 rttomo pombe, wt and septin delete 4 also deleted and made new cschwart
account
Thu Jul 16 17:33:43 2009 cedric 07/16 10:00 AM 17:30 PM tiltrotate rttomo polyoma-dextran-plastic sections 3
Fri Jul 17 16:04:07 2009 morphew 7-17 9 AM 4 PM 70 rttomo low temp freeze sub 2
Mon Jul 20 10:54:32 2009 cindi 07/20 10:00 AM 10:45 AM standard rtimage giardia hexadecene column vacuum at 17 this morning
Mon Jul 20 19:10:47 2009 Johanna 07/20 11:00 AM 7:30 PM fishione rttomo tryps telophase + leish 5 Can still not transfer files to
simba.
Tue Jul 21 16:51:03 2009 morphew 7/23 1 PM 5 PM 70 rttomo low temp freeze sub 1
Wed Jul 22 16:35:19 2009 cedric 07/21 11:00 AM 16:30 PM f30cryo cryoimaging pombe 0 this scope sucks anyhow. Darn it ...
Thu Jul 23 16:35:15 2009 cedric 07/23 10:00 AM 16:30 PM 914 cryoimaging pombe 0 I defenetly hate this microscope.
beam is always shifting. Objectif
aperture always get in the way. it
sucks the dose calibration should some
how be skrewed up because now to
take a decent picture I should use
more than 20 e/A2.
Fri Jul 24 15:57:40 2009 eileen 7/24 8 AM 4 PM fishione rttomo wt centrosome 6
Tue Jul 28 16:07:26 2009 philip/cindi 07/28 9:15 AM 4:00 PM 70 rttomo aptamers on cells 9 training went well
Wed Jul 29 16:12:13 2009 cindi 7/29 3:00 PM 4:15 PM standard rtimage giardia x-sxns
Thu Jul 30 14:08:40 2009 Kristin 07/30 9:00 AM 2:00 PM fishione rttomo Trypanosomes 2
Fri Jul 31 16:09:09 2009 cedric 07/31 09:00 AM 16:00 PM f30cryo cryoimaging pombe 0 defenetly sucks :)))
Wed Aug 5 18:52:49 2009 Matthew West 08/05 8;15 AM 7:00 PM fishione rttomo ER mutant, Voeltz lab 14 Gia Voeltz lab strain
Thu Aug 6 13:33:52 2009 morphew 8-6 9 AM 1:30 PM f30cryo cryoimaging mt's in vivo 1
Thu Aug 6 16:08:43 2009 Kristin 08/06 2:10 PM 4:05 PM fishione rtimage pombe 0
Mon Aug 10 18:58:11 2009 Nesia 09/10 9:00 AM 7:00 PM tiltrotate rttomo HM20 embedded yeast 12
Tue Aug 11 16:08:13 2009 Nesia 09/11 10 AM 4 PM tiltrotate rttomo HM20 yeast 8
Wed Aug 12 15:39:03 2009 morphew 8/12 9 AM 3:30 PM f30cryo cryotomo mechanosensitive channel 4
Thu Aug 13 15:52:56 2009 Johanna 08/13 10:00 AM 4:00 PM fishione rttomo leishmania 4 still can not transfer data without
ssh-ing them.
Fri Aug 14 12:17:18 2009 Johanna 08/14 08:30 AM 12:00 PM fishione rttomo LEISHMANIA 6
Mon Aug 17 12:13:43 2009 Janet Meehl/Winey 8/17 9:15 AM 12:15 PM tiltrotate rttomo WTtet basal bodies, duplicating 2
Tue Aug 18 17:33:27 2009 Janet Meehl/Winey 8/18 10:00 AM 5:45 PM tiltrotate rttomo WTtet duplicating basal bodies 9
Wed Aug 19 12:07:33 2009 Johanna 08/19 11:00 AM 12:00 PM fishione rttomo leishmania 0 serial sections
Wed Aug 19 18:30:12 2009 Janet Meehl/Odde 819 12:15 PM 6:45 PM tiltrotate rttomo WTtetrahymena duplicating basal
bodies 8
Thu Aug 20 16:20:01 2009 Kristin 08/20 2:20 PM 5:00 PM fishione rttomo pombe 2
Fri Aug 21 18:44:37 2009 cedric 08/21 9:00 AM 19:00 PM tiltrotate rttomo polyoma-38h-mannitol 8
Tue Aug 25 16:41:16 2009 morphew 8-25 9 AM 5 PM tiltrotate rttomo bpv 4
Wed Aug 26 17:49:58 2009 Robert Kirmse 08/26 10:00 AM 5:50 PM f30cryo cryoimaging CEMOVIS hDesWT 0
Thu Aug 27 10:38:06 2009 Cindi 08/27 10:15 AM 10:30 AM standard serviceman checking out GIF alignments Checked out GIF alignments b/c
Robert had issues yesterday. He had
a dark corner all the time with all
apertures and sample out. My
account is fine.
Fri Aug 28 15:43:05 2009 cedric 08/28 10:00 AM 16:00 PM f30cryo cryoimaging section-polyoma-38h 0
Mon Aug 31 17:20:28 2009 Nesia Zurek 8/31 9:00 AM 5:30 PM fishione rttomo HM20 embedded Yeast 12 besides resetting goniometer... it
went perfectly reset goniometer
Tue Sep 1 16:31:24 2009 morphew 9-1 9 AM 4:30 PM f30cryo cryotomo mpp 2
Thu Sep 3 17:30:04 2009 cedric 09/03 10:00 AM 17:30 PM f30cryo cryoimaging yeast-section 0
Fri Sep 4 15:54:03 2009 eileen 9/4 8:15 AM 4:00 PM fishione rttomo DeCamili; endophilin TKO;Dynamin KO 6ts (4 2x1) Please leave scope with standard
apertures
Tue Sep 8 17:35:39 2009 eileen 9/8 8:15 AM 5:30 PM fishione rttomo DeCamili; dynaminKO_Kstimulation 10 vacuum took a while to come down
after inserted rod.
Wed Sep 9 16:14:02 2009 cindi 09/09 9:00 AM 4:00 PM tiltrotate rttomo giardia thick sxns vacuum very slow coming down
Thu Sep 10 14:11:15 2009 DNM 9/10 11:30 AM 2:15 PM 70 rtimage Distortion calubration for 13.5 and
9.3K Do not ever do this without the
rotating rod
Fri Sep 11 15:35:32 2009 eileen 9/11 8:30 AM 3:30 PM fishione rttomo DeCamili dynamin KO recovery 1 min 8
Sun Sep 13 15:27:38 2009 cindi 09/13 12:30 PM 3:30 PM tiltrotate rttomo giardia serials 1
Mon Sep 14 17:10:13 2009 eileen 9/14 8:15 AM 5:00 PM fishione rttomo Platelets with Brian Storrie vac stayed ~12 even though oring
had new grease
Wed Sep 16 14:42:54 2009 Nesia 9/16/09 9:30 AM 3:00 PM fishione rttomo Yeast in HM20 6 none
Mon Sep 21 17:13:32 2009 eileen 9/21 9 AM 5:10 PM fishione rttomo Brian Storrie Platelets 3
Tue Sep 22 19:22:25 2009 Matthew West 09/22 9:15 AM 7:25 PM fishione rttomo Yeast ER / Endosomes Voeltz time: 8 hrs
Odorizzi time: 2 hrs
Wed Sep 23 16:58:13 2009 morphew 9-23 9:30 AM 9 PM tiltrotate rttomo bpv 2
Thu Sep 24 15:14:09 2009 morphew 9-26 9 AM 3:10 PM tiltrotate rttomo bpv 4
Fri Sep 25 15:39:19 2009 Nesia Zurek 9/25 9:30 AM 3:30 PM fishione rttomo HM20 yeast 6 good
Mon Sep 28 16:26:27 2009 eileen 9/28 9 AM 4:20 PM tiltrotate rttomo eyespot 4
Tue Sep 29 17:17:06 2009 cedric 09/29 10:00 AM 18:00 PM fishione rttomo polyoma-dextran 4 smooth
Wed Sep 30 19:43:51 2009 cedric 09/30 10:00 AM 20:00 PM fishione rttomo polyoma-dextran-36H 3 worked good
Thu Oct 1 15:47:34 2009 Nesia 10/01 10 AM 4 PM fishione rttomo HM20 embedded yeast 12 Cedric was still logged on when i
came in, not a big deal though none
Thu Oct 1 16:40:53 2009 Cindi 10/01 4:00 PM 4:40 PM tiltrotate rttomo giardia 400nm sxns 1 just testing :o)
Fri Oct 2 10:04:57 2009 Cindi 10/02 9:00 AM 10:00 AM tiltrotate rttomo thick sections giardia 1 good
Fri Oct 2 17:04:27 2009 morphew 10-2-09 10 AM 5 PM tiltrotate rttomo marcus engstler tryps 4-3x1
Mon Oct 5 12:50:23 2009 morphew 10-5 9 AM 12;45 PM tiltrotate rttomo ENGSTLER TRYPS 2 3X1
Mon Oct 5 19:55:11 2009 Matt Russell Oct 5 1:30 PM 8:00 PM tiltrotate rttomo yeast plastic 3 Vacuum IGP1 was increasing (up to
~40) during tilt series. Matt W
helped me remove rod and clean o-
ring, and fill up cold finger
dewar. IGP1 vacuum allowed to
return to 15. Replaced rod,
resumed tilt series; vacuum OK.
Tue Oct 6 12:58:35 2009 eileen 10/6 8:15 AM 12:50 PM fishione rttomo EndTKODynKo DeCamilli 3 1x2
Tue Oct 6 18:28:00 2009 Matt Russell Oct 6 1:30 PM 6:30 PM tiltrotate rttomo yeast plastic 2
Wed Oct 7 09:55:17 2009 eileen 10/7 8:15 AM 9:45 AM fishione rttomo EndophilinTKO/dynaminKO DeCamilli 1 1x2
Wed Oct 7 17:20:34 2009 cedric 10\07 10:00 AM 17:30 PM fishione rttomo polyoma-dextran 12
Thu Oct 8 16:13:28 2009 Kristin 10/08 12:30 PM 4:15 PM fishione rttomo pombe 2
Fri Oct 9 13:27:14 2009 eileen 10/8 8:15 AM 1:20 PM fishione rttomo Endophilin TKO DeCamilli 3 o-ring on fishione was bad- this
has been causing vac problems.
Column dumped this am. Ron replaced
and now no vac problems.
Fri Oct 9 18:51:33 2009 Matt Russell 10/09 2:00 PM 6:45 PM tiltrotate rttomo yeast plastic 2
Mon Oct 12 18:49:40 2009 Janet Meehl/Winey 10/12 12:30 PM 6:50 PM tiltrotate rttomo WTtet assembling basal bodies 6
Tue Oct 13 17:17:12 2009 eileen 10/13 8:15 AM 5:15 PM fishione rttomo Odde MTs and gamma RNAi 9
Wed Oct 14 16:48:16 2009 cynthia 10/14 9:30 AM 4:45 PM f30cryo cryoimaging ifs used the gif
Thu Oct 15 14:06:12 2009 Kristin 10/15 9:30 AM 2:00 PM fishione rttomo pombe 2
Fri Oct 16 10:00:53 2009 Janet Meehl Fox/Winey 10/16 8:45 AM 10:00 AM tiltrotate rttomo WT tet basal bodies 0 formvar on grid broke
Fri Oct 16 15:51:19 2009 eileen 10/15 9:15 AM 3:45 PM fishione rttomo Odde MTs and gamma RNAi 6 Please leave microscope in standard
condition
Mon Oct 19 17:04:23 2009 Robert Kirmse 10/19 10 AM 16 PM f30cryo cryoimaging Desmin Tetramers 0 Scope was good
samples were shit
Wed Oct 21 17:43:48 2009 Kristin 10/21 1:00 PM 6:00 PM fishione rttomo pombe 2
Thu Oct 22 19:28:57 2009 cynthia 10/21 9:30 AM 7:30 PM f30cryo cryotomo ifs 2 worked well! Hi Cindi, only problem here, I saw
the upper right quarter panel in my
images.
Fri Oct 23 16:15:12 2009 eileen 10/23 8:15 AM 4:10 PM fishione rttomo Odde LLC-PK1 5 (2x2 and 1x1)
Mon Oct 26 17:21:17 2009 morphew 10-26 9 AM 5:30 PM f30cryo cryotomo bpv 5 dumped column at rod removal--again!
Tue Oct 27 16:28:41 2009 eileen 10/27 8:10 AM 1:15 PM tiltrotate rttomo Odde LLC-PK1 3 2x2's
Tue Oct 27 16:42:37 2009 morphew 10-27 1 PM 5 PM tiltrotate rttomo hela midbody 2 2x1
Wed Oct 28 14:46:15 2009 cynthia 10/28 9:15 AM 2:45 PM f30cryo cryoimaging ifs except for the occasional upper
right quarter panel, aok!
Thu Oct 29 18:08:44 2009 Johanna/Neil 10/29 8:30 AM 6:30 PM fishione rttomo trypanosome collar 10 everything perfect!
Fri Oct 30 19:02:31 2009 Johanna/Neil 10/30 08:30 AM 7:30 PM fishione rttomo trypanosome collar 10
Sat Oct 31 17:26:21 2009 Johanna/Neil 10/31 9:00 AM 5:00 PM fishione rttomo Trypanosome collar 8
Sun Nov 1 19:16:02 2009 Johanna/Neil 11/1 09:00 AM 7:15 PM fishione rttomo Trypanosome collar 9
Mon Nov 2 12:02:18 2009 Kristin 11/2 8:30 AM 12:00 PM fishione rttomo pombe 2
Mon Nov 2 21:09:29 2009 Johanna/Neil 11/2 12:30 PM 9:15 PM fishione rttomo trypanosome collar 10
Tue Nov 3 12:36:21 2009 Johanna/Neil 11/3 8:00 AM 12:30 PM fishione rttomo trypanosome collar 7
Wed Nov 4 09:57:18 2009 sRon 11/04 8:00 AM 10:00 AM standard serviceman HT off refilled water chiller. brought
scope back up. reset goniometer
Wed Nov 4 10:08:04 2009 Kristin 11/3 12:30 PM 4:30 PM tiltrotate rttomo HeLa 1
Wed Nov 4 15:57:08 2009 eileen 11/4 10:30 AM 3:50 PM tiltrotate rttomo Odde MTs 2 2x2 microscope vac down when I came
in.Ron got microscope up and
running.
Thu Nov 5 17:57:08 2009 Janet Meehl Fox/Winey 11/5 8:30 AM 6:00 PM tiltrotate rttomo poc1del tet and WT tet dupl. bbs 10 I don't know if it was the specimen
rod or the stage motor that was
randomly clicking on my last TS. I
had to wiggle the specimen rod to
get it to move back to center. It
seems quiet with the std. rod in.
My last TS also seized up a couple
of times which I think was the
specimen rod/stage issue.
Fri Nov 6 13:57:08 2009 cindi 11/06 9:30 AM 11:45 AM f30cryo cryoimaging giardia samples too thick
Fri Nov 6 15:28:41 2009 cindi 11/06 11:45 AM 3:30 PM tiltrotate rttomo giardia dextran beads 2 cells looked good! had to reload alignments--weird
rotation in LMM. also, accidentally
unplugged computer, so it got
rebooted.
Sun Nov 8 18:35:40 2009 DNM 11/8 2:30 PM 6:30 PM standard development Testing drift compensation with
image shift
Mon Nov 9 15:14:48 2009 morphew 11-9 9 AM 3 PM tiltrotate rttomo bpv 2
Mon Nov 9 17:12:48 2009 Johanna/Neil 9/11 15:15 PM 17:15 PM fishione rttomo trypanosome collar 2
Mon Nov 9 18:33:33 2009 DNM 11/9 5:30 PM 6:30 PM standard development Drift compensation with image shift
Tue Nov 10 16:44:27 2009 cindi 11/10 9:15 AM 4:45 PM tiltrotate rttomo giardia 4 some montaging tests
Wed Nov 11 15:12:12 2009 Janet Meehl Fox/Winey 11/11 9:00 AM 3:20 PM tiltrotate rttomo WTtet duplicating basal bodies 8
Thu Nov 12 12:33:07 2009 Johanna 11/12 9:15 AM 12:30 PM fishione rttomo tryps 3
Thu Nov 12 17:26:43 2009 Kristin/John 11/12 12:30 PM 5:30 PM tiltrotate rttomo hela telophase 2 montages
Thu Nov 12 18:39:30 2009 DNM 11/12 5:30 PM 6:45 PM standard development Recording shifting image during
exposure on GIF video camera Yes, there is vibration in the GIF
Fri Nov 13 15:56:32 2009 DNM 11/13 3:25 PM 4:00 PM standard development more drift image shift measurements
Fri Nov 13 18:26:39 2009 Johanna 11/13 4:00 PM 6:30 PM fishione rttomo trypanosome 3
Mon Nov 16 17:34:02 2009 Matthew West 11/16 9:00 AM 5:30 PM tiltrotate rttomo Yeast endosomes, Bro1 oe 10 This work was for Greg Odorizzi's
lab.
Tue Nov 17 18:00:26 2009 eileen 11/17 8:30 AM 6:00 PM fishione rttomo C.elegans SPD5 RNAi 10
Wed Nov 18 17:51:04 2009 Robert Kirmse 11/18 9:30 AM 6:00 PM f30cryo cryoimaging desmin 0 no problems
Thu Nov 19 15:39:36 2009 morphew 11-19 8:30 AM 3:30 PM f30cryo cryotomo s9 cells 0
Fri Nov 20 16:19:33 2009 cynthia 11/19 9:30 AM 4:00 PM f30cryo cryoimaging if Seems to be a bug in Refine ZLP
according to David
Mon Nov 23 16:56:21 2009 cynthia 11/23 10:00 AM 5:00 PM f30cryo cryoimaging ifs very good!
Tue Nov 24 18:03:39 2009 eileen 11/24 9:00 AM 6:00 PM fishione rttomo SPD5 RNAi and Dyn1KO recovery 8
Wed Nov 25 15:55:44 2009 morphew 11-26-09 8:30 AM 4 PM f30cryo cryotomo c127 bpv 4
Sat Nov 28 14:28:53 2009 Johanna 11/28 9:00 AM 2:30 PM fishione rttomo trypanosome collar 6
Tue Dec 1 16:34:55 2009 Johanna 12/1 11:00 AM 4:30 PM fishione rttomo trypanosome fusion 6 left myself logged in so that the
files could transfer.
Thu Dec 3 21:09:39 2009 cedric 12/03 10:00 AM 21:30 PM tiltrotate rttomo polyoma 10
Fri Dec 4 15:49:43 2009 cindi 12/04 9:00 AM 3:45 PM tiltrotate rttomo Giardia sections Low Temp 6
Mon Dec 7 14:30:31 2009 morphew 12-7 9 AM 12 AM f30cryo cryotomo s9 0
Mon Dec 7 18:00:23 2009 Johanna 12/7 2:30 PM 6:00 PM fishione rttomo trypanosome fusion 2
Tue Dec 8 17:28:49 2009 Matthew West 12/08 10:15 AM 5:45 PM tiltrotate rttomo Bro1p over-expressed yeast 8 Lower than normal beam energy for
spot size 3 so I used spot size 2,
otherwise good alignments.
Wed Dec 9 18:30:00 2009 Kirmse Robert 12/09 09:00 AM 7:00 PM f30cryo cryoimaging cryo negativ staining test 0 All good during session,
crahed vacuum, while reinserting
RT-holder
Thu Dec 10 13:08:02 2009 Johanna 12/10 9:30 AM 1:00 PM fishione rttomo tryps fusion 1
Thu Dec 10 16:22:51 2009 Kristin 12/10 1:00 PM 5:00 PM tiltrotate rttomo HeLa 2
Fri Dec 11 15:24:29 2009 cedric 12/11 9:30 AM 16:00 PM tiltrotate rttomo polyoma virus 1
Mon Dec 14 18:21:43 2009 Janet Meehl Fox/Winey 12/14 9:00 AM 6:30 PM tiltrotate rttomo Tet poc1del bbs 12
Tue Dec 15 11:31:54 2009 eileen 12/15 8:50 AM 11:30 AM fishione rttomo spd5 RNAi 2
Tue Dec 15 17:35:58 2009 cindi 12/15 12:00 PM 5:30 PM tiltrotate rttomo giardia 6
Wed Dec 16 17:14:40 2009 Kirmse Robert 12/16 9:30 AM 5:30 PM f30cryo cryoimaging Desmin 0 all good
Thu Dec 17 16:43:17 2009 Kristin 12/17 9:00 AM 5:00 PM tiltrotate rttomo HeLa 6
Fri Dec 18 16:35:16 2009 eileen 12/18 9:15 AM 4:30 PM fishione rttomo spd5 RNAi 2
Mon Dec 21 18:08:18 2009 Matthew West 12/21 8:30 AM 6:30 PM tiltrotate rttomo WT & Bro1 over expression 14 Odorizzi lab
Tue Dec 22 16:26:58 2009 cindi 12/22 10:00 AM 4:30 PM tiltrotate rttomo giardia 5
Sun Dec 27 15:24:55 2009 Cindi 12/27 10:00 AM 3:00 PM tiltrotate rttomo giardia 5 The e: drive is sounding very
strange. Cleaned out the drive and
Dave is going to send it back for
repair.
Mon Dec 28 16:58:42 2009 cindi 12/28 9:00 AM 5:00 PM tiltrotate rttomo Giardia 6 camera vacuum high. chuck to come
fix this wednesday.
Tue Dec 29 14:46:51 2009 eileen 12/29 9:30 AM 2:40 PM fishione rttomo Chlamy bb duplication 6
Tue Dec 29 16:15:47 2009 cindi 12/29 3:00 PM 4:00 PM tiltrotate rttomo giardia 1
Wed Dec 30 12:56:07 2009 Chuck Moe 12/30 9:00 AM 11:00 AM standard serviceman fix camera vacuum cleaned seals/orings. Penning guage
bad. Ordered penning guage, to be
replaced on Monday.
Wed Dec 30 15:56:06 2009 Cindi 12/30 2:00 PM 4:00 PM tiltrotate rttomo Giardia 2 Had to wait for cameras to cool
down. Had to shut down ultracam and
restart. Chuck also lubed the
binocs.
Thu Dec 31 10:51:19 2009 Cindi 12/31 8:00 AM 10:45 AM tiltrotate rttomo Giardia 3
Thu Dec 31 16:38:54 2009 Kristin 12/31 11:00 AM 4:45 PM tiltrotate rttomo HeLa 4
Tue Jan 5 17:23:38 2010 eileen 1/5 8:40 AM 5:20 PM fishione rttomo chlamy bb, spd5 rnai, llcpk1 mts 4 + 3 2x2 put the scope in standby mode after
session for power outage
Wed Jan 6 14:23:21 2010 Ron Spilman 1/6 8:40 AM 5:00 PM standard serviceman Preventive maintenance Replace buffer hose, prevac hose,
vac oil. Replace red soop lamp.
Drain water from Jun-Air. Made
electronic and vacuum checks as per
PM check list.
Thu Jan 7 15:03:25 2010 Kristin 1/7 9:00 AM 3:15 PM tiltrotate rtimage trypanosomes 0
Mon Jan 11 18:59:19 2010 Matthew West 01/11 10:30 AM 7:00 PM tiltrotate rttomo Snf7 point mutants and WT yeast 12
Tue Jan 12 13:32:37 2010 eileen 1/12 8:30 AM 1:30 PM fishione rttomo WT gamma-GFP controls 5
Wed Jan 13 13:21:09 2010 morphew 1-13-10 9 AM 1 PM tiltrotate rttomo low temp freeze sub 4
Wed Jan 13 16:56:02 2010 Kristin 1/13 1:30 PM 5:30 PM tiltrotate rttomo trypanosomes 2
Thu Jan 14 17:30:26 2010 Kirmse Robert 01/14 9:00 AM 5:30 PM f20cryo cryoimaging cryo negative stain 0
Tue Jan 19 17:27:46 2010 eileen 1/19 8:50 AM 5:30 PM fishione rttomo spd5 RNAi and Odde mts 6 ts + 2 2x2 ts
Wed Jan 20 18:12:08 2010 Kristin 1/20 7:50 AM 6:30 PM tiltrotate rttomo trypanosomes 8
Fri Jan 22 10:02:12 2010 Ron Spilman 1/22 8:30 AM 10:15 AM standard serviceman Service (Replace PV pump)
Replace prevac pump pump was slow starting ok now
Fri Jan 22 17:36:00 2010 cedric 01/22 10:30 AM 17:30 PM f30cryo cryoimaging sections 0
Mon Jan 25 18:22:22 2010 Johanna 01/25 11:00 AM 6:30 PM fishione rttomo trypanosomes 5 montages
Tue Jan 26 11:34:44 2010 Kristin 1/26 8:30 AM 11:30 AM tiltrotate rtimage hela 0
Tue Jan 26 17:56:00 2010 Johanna 01/26 01:00 PM 6:00 PM fishione rttomo trypanosomes 5 2 x1 montages
Wed Jan 27 16:50:16 2010 morphew 1-27 8:30 AM 5 PM f30cryo cryotomo polyoma 3 I don't think the cryobox is
working properly.
Thu Jan 28 16:55:55 2010 Kristin 1/28 8:30 AM 5:00 PM tiltrotate rttomo hela 6
Fri Jan 29 11:44:25 2010 eileen 1/29 8:15 AM 11:40 AM fishione rttomo De Camilli WT Recov. 1 min 4
Fri Jan 29 15:58:49 2010 morphew 1-29 12 PM 4 PM fishione rttomo polymom virus 3
Mon Feb 1 15:58:11 2010 morphew 2-1-10 9 AM 4 PM fishione rttomo polyoma 4 2x1
Tue Feb 2 14:45:54 2010 Kristin 2/2 8:15 AM 2:45 PM tiltrotate rttomo hela 3 crashed vacuum, computer lost
communication with scope
Wed Feb 3 19:41:22 2010 Johanna 2/4 10:00 AM 7:45 PM fishione rttomo trypanosome collar 2
Fri Feb 5 15:02:12 2010 ghoshn 02/05 1:45 PM 3:00 PM standard training training
Fri Feb 5 15:29:13 2010 cschwart 02/05 3:00 PM 3:30 PM 70 rtimage checking grids, they sucked
Mon Feb 8 14:37:41 2010 ghoshn 02/08 1:00 PM 2:32 PM standard rtimage training
Tue Feb 9 15:59:25 2010 Kristin 2/9 11:30 AM 4:30 PM tiltrotate rttomo hela 2
Wed Feb 10 17:16:43 2010 Kristin 2/10 8:30 AM 5:30 PM tiltrotate rttomo hela 4
Thu Feb 11 12:51:09 2010 Kristin 2/11 9:00 AM 1:00 PM tiltrotate rttomo hela 2
Fri Feb 12 15:11:56 2010 cedric 02/12 12:30 PM 15:30 PM f20cryo cryoimaging sections 0
Mon Feb 15 13:50:30 2010 morphew 2-15 8:15 AM 1:45 PM f30cryo cryotomo mpp 1
Tue Feb 16 18:09:44 2010 eileen 2/16 8:50 AM 6:00 PM tiltrotate rttomo C.elegans spd5; chlamy flagella 10
Wed Feb 17 17:03:37 2010 eileen 2/17 8:20 AM 5:00 PM tiltrotate rttomo chlamy flagella 4
Thu Feb 18 17:12:05 2010 cindi 02/18 9:00 AM 5:00 PM f30cryo cryotomo isolated giardia cytoskeletons 7
Fri Feb 19 17:09:24 2010 Robert Kirmse 2/19 9:30 AM 5:00 PM f30cryo cryoimaging Desmin 0
Mon Feb 22 14:22:28 2010 Nesia Zurek 2/22 10 AM 2 PM fishione rttomo yeast HM20 4 no none
Mon Feb 22 19:40:45 2010 Matthew West 02/22 3:00 PM 7:45 PM tiltrotate rttomo Voeltz lab, ER mutant 6 Voeltz lab Serial EM was a little strange;
worked better after restarting
program
Tue Feb 23 16:44:14 2010 eileen 2/23 8:30 AM 4:40 PM tiltrotate rttomo Odde Ase1 spindles and chlamy
flagella maps 8 extraction voltage was below 4000
Wed Feb 24 21:39:22 2010 Cindi/Moises 02/24 12:00 PM 2:00 PM f30cryo cryoimaging giardia cytos
Wed Feb 24 21:39:45 2010 Cindi/Moises 02/24 2:00 PM 9:30 PM tiltrotate rttomo giardia 5
Thu Feb 25 17:58:53 2010 Cindi/Moises 02/25 10:20 AM 6:00 PM tiltrotate rttomo Giardia 9
Fri Feb 26 17:50:05 2010 cindi/Moises 02/26 9:00 AM 3:00 PM f30cryo cryoimaging giardia cytos samples sucked
Fri Feb 26 17:50:24 2010 cindi/Moises 02/26 3:00 PM 5:45 PM tiltrotate rtimage giardia
Sat Feb 27 17:37:13 2010 cindi/moises 02/27 10:00 AM 5:30 PM f30cryo cryotomo giardia cytos 2
Mon Mar 1 16:43:37 2010 Cindi/Moises 03/01 9:00 AM 11:30 AM tiltrotate rttomo giardia 2
Mon Mar 1 16:43:54 2010 Cindi/Moises 03/01 11:30 AM 4:30 PM f30cryo cryoimaging giardia cytos samples too thick
Mon Mar 1 16:48:03 2010 Cindi 03/01 4:45 PM 4:46 PM standard serviceman Warm-up UltraCam Warmed up Ultra-Cam overnight.
Tue Mar 2 16:34:08 2010 eileen 3/2 8:30 AM 4:30 PM tiltrotate rttomo Odde Ase1 yeast 10
Wed Mar 3 11:37:00 2010 cindi 03/03 9:00 AM 11:30 AM standard serviceman quadrant correction UltraCam Quadrant correction UltraCam
Wed Mar 3 21:47:59 2010 cindi/moises 03/03 12:30 PM 9:45 PM f20cryo cryotomo giardia cytos 6
Thu Mar 4 18:59:14 2010 cindi/moises 03/04 2:00 PM 7:00 PM f20cryo cryotomo giardia WT cytos 4
Fri Mar 5 12:37:35 2010 morphew 3/5 9 AM 12:30 PM tiltrotate rttomo polyoma virus 2
Fri Mar 5 17:15:26 2010 cindi/moises 03/05 2:30 PM 4:30 PM tiltrotate rttomo giardia chem fix 2
Tue Mar 9 17:37:50 2010 eileen 3/9 8:30 AM 5:15 PM tiltrotate rttomo chlamy flagella 4
Thu Mar 11 15:00:53 2010 DNM 3/11 2:35 PM 3:00 PM standard rtimage looking at aperture sizes, getting
carbon film pictures
Fri Mar 12 15:01:02 2010 morphew 3/13 8:30 AM 3 PM tiltrotate rttomo aditi neurons 0
Mon Mar 15 17:06:13 2010 Cindi 03/15 2:30 PM 5:00 PM standard training making maps writing paper on how to make
various maps
Tue Mar 16 12:32:16 2010 Robert Kirmse 03/16 10:00 AM 1:00 PM 914 cryoimaging Vitrobot test 0
Wed Mar 17 14:06:13 2010 DNM 3/17 2:00 PM 2:15 PM standard rtimage New noise files for ultracam
Thu Mar 18 15:38:32 2010 cindi 03/18 10:00 AM 1:00 PM standard serviceman vacuum bad vacuum was at 30 at logon. taking
holder in and out didn't help.
called service. suggested to
replace o-ring and leave holder out
for awhile. vacuum slowly started
dropping. put holder in/out a few
times and vacuum stayed at 6 all
day. chuck to come by in the
morning and double-check.
Fri Mar 19 09:08:56 2010 Chuck Moe 3/19 08:30 AM 09:30 AM standard serviceman checked stage and column vacuum checked stage and column vacuum found no vacuum problems
Mon Mar 22 16:25:17 2010 Workshop 03/22 10:00 AM 4:15 PM 914 training Workshop demo
Fri Mar 26 14:28:06 2010 Cindi 03/26 9:00 AM 2:00 PM f20cryo cryotomo Giardia 4 seems like the camera vacuum may be
spiking when the slit goes in/out.
Mon Mar 29 14:57:30 2010 spilman 3/29 8:00 AM 17:00 PM standard serviceman service vacuum issues after weekend.
Appears to be V5.
Replace 100u C2 aperture.
Tue Mar 30 14:31:49 2010 Spilman 3/30 8:00 AM 2:30 PM standard serviceman service vacuum issues with single tilt
holder.
Tue Mar 30 16:54:41 2010 cindi 03/30 3:00 PM 5:00 PM tiltrotate rttomo Giardia 2
Wed Mar 31 08:33:34 2010 Matthew West 3/24 9:00 AM 7:00 PM tiltrotate rttomo ER triple delta 12 The camera insertion trick works! The cryo box was still in
preventing me from acquiring the
beam and delaying my work. Cindi
pointed it out and I should be able
to fix future cryo box problems
Wed Mar 31 11:24:36 2010 morphew 03/31/10 8:30 AM 11:30 AM tiltrotate rttomo aditi neurons 2 1x2
Thu Apr 1 14:33:26 2010 Kristin 4/1 8:00 AM 2:30 PM tiltrotate rttomo hela 3
Mon Apr 5 14:50:40 2010 Kristin 4/5 8:00 AM 3:00 PM tiltrotate rttomo hela 4
Tue Apr 6 16:22:51 2010 eileen 4/6 8:45 AM 4:20 PM tiltrotate development supermontage test runs with David 1 3x3 plus test runs
Thu Apr 8 17:43:35 2010 Johanna/Cindi 04/08 09:00 AM 5:30 PM f30cryo cryotomo Trypanosomes whole cells 2 Vaccum was at 24 in the morning.
Fri Apr 9 16:40:40 2010 eileen 4/9/10 8:00 AM 4:40 PM 70 rttomo 2x2 supermontage (3x3 panels) auto
acquisiton with macro- YEA!!!! 4 3x3 montage vac was at 6 this morning.
Mon Apr 12 08:09:56 2010 Spilman 4/12 8:00 AM 8:15 AM standard serviceman none check vacuum vacuum at log 6
Mon Apr 12 16:51:31 2010 eileen 4/12 9 AM 4:45 PM tiltrotate rttomo supermontage aquisition with
Needleman 2 3x3 column vac at 6 after the weekend!
Tue Apr 13 12:38:34 2010 Nesia 4/13 10 AM 12:30 PM tiltrotate rttomo HM20 Yeast 1 trouble engaging rod in airlock
Wed Apr 14 11:18:34 2010 mo0rphew 4-13 8:30 AM 11:30 AM tiltrotate rttomo aditi neurons 2 2x1
Thu Apr 15 21:09:38 2010 Johanna 15/4 9:30 AM 9:30 PM fishione rttomo Trypanosome collar 10 At one point, an x-ray warning came
up saying it was not safe. It
disappeared by itself.
Mon Apr 19 16:20:52 2010 eileen 4/19 11:00 AM 4:15 PM fishione rttomo DynaminKO 30min recovery (DeCamilli) 6
Tue Apr 20 16:36:25 2010 Johanna/Cindi 04/20 9:30 AM 16:30 PM f30cryo cryotomo trypanosomes whole mount/lysed 1
Wed Apr 21 16:15:19 2010 morphew 4-21 8:15 AM 4:15 PM tiltrotate rttomo polyoma, aditi neurons 4
Thu Apr 22 12:56:43 2010 Nesia 4/22 9:30 AM 1 PM tiltrotate rttomo HM20 yeast 4
Thu Apr 22 16:38:13 2010 Matt Russell 04/22 1:30 PM 4:30 PM tiltrotate rtimage Practice, checking sample
Fri Apr 23 15:08:47 2010 Cindi 04/23 9:00 AM 3:00 PM standard rtimage EELS warmed upper camera ZLP not centered between imaging
and spectroscopy mode. Need ALOT of
dose for EFTEM imaging. Beam moving
as eV changes. Sent email to Dan
Ray from Gatan. Warmed upper camera
Mon Apr 26 18:54:25 2010 Johanna 04/26 9:30 AM 7:00 PM f30cryo cryotomo trypanosome detergent extracted
flagella 8 bad vaccum in the morning.
Tue Apr 27 16:37:20 2010 eileen 4/27 8:30 AM 4:30 PM tiltrotate rttomo spindle supermontage and synapses
(decamili) 1x2 supermontage (3x3s) and 3 other ts vac at 6 this morning.
Wed Apr 28 17:26:12 2010 Johanna 04/28 9:00 AM 5:30 PM f30cryo cryotomo whole cell tryps 3
Thu Apr 29 18:06:17 2010 Kirmse Robert 04/29 10:00 AM 6:00 PM f30cryo cryotomo desmin 1 put the camera in before opening
the c-valves but in my case the
vaccum didn't spike and stayed at 6
Fri Apr 30 12:48:54 2010 spilman 4/30 8:00 AM 1:00 PM standard serviceman Vibration issues Resolved issue
Mon May 3 12:49:42 2010 eileen 5/3 8:30 AM 12:30 PM fishione rttomo WT neurons 30 min recovery
(DeCamili) 3 column vac at 32 when I came in. Hey Cindi-
column vac was at 32 when I came
in. Took ~ hour to come down. Also,
no e drive available. Saved to 'my
document' folder.
Mon May 3 13:04:23 2010 cindi 05/03 1:00 PM 1:01 PM standard serviceman turned on/off external drive external drive was not recognized.
turned off, then on and now
recognized
Wed May 5 16:11:16 2010 Robert Kirmse 05/03 9:15 AM 04:10 PM f30cryo cryoimaging Desmin 0 Vaccum was at 26 in the morning
Fri May 7 20:50:21 2010 cedric 05/07 10:00 AM 21:00 PM f30cryo cryotomo section 4
Mon May 10 14:51:24 2010 Spilman 5/10 8:00 AM 3:00 PM standard serviceman service 0 Replaced V5 Vacuum deteriates over long period
of time after cryo cycle when turbo
is off
Tue May 11 16:07:20 2010 morphew 5-11 1:30 PM 4:15 PM tiltrotate rttomo heart muscle 2
Wed May 12 15:43:12 2010 morphew 5-13 8:30 AM 3:30 PM tiltrotate rttomo cardiac myocytes 3
Thu May 13 11:52:47 2010 morphew 5-13 9 AM 12 AM tiltrotate rttomo cardiac myocytes 2 2x1
Thu May 13 15:29:42 2010 Robert Kirmse 05/13 1:00 PM 4:00 PM f30cryo cryoimaging Desmin 0
Fri May 14 15:41:51 2010 morphew 5-14 9 AM 4 PM tiltrotate rttomo cardiac myocyte 4
Mon May 17 15:36:32 2010 morphew 5-17 8:30 AM 3:30 PM tiltrotate rttomo cardiac myocyte 4 2x1
Wed May 19 16:50:56 2010 Kristin 5/19 1:30 PM 4:45 PM standard rtimage neurons 0
Thu May 20 12:33:07 2010 Janet Meehl Fox/Winey 5/20/10 9:30 AM 12:30 PM tiltrotate rttomo poc1del, WT tet basal bodies 2
Fri May 21 16:40:57 2010 Robert Kirmse 05/21 9:00 AM 5:00 PM f30cryo cryoimaging Desmin 0
Mon May 24 16:55:12 2010 eileen 5/24 8:30 AM 5:00 PM tiltrotate rttomo Chlamy flagella/bbs 8
Tue May 25 14:24:46 2010 Kristin 5/25 8:45 AM 2:20 PM tiltrotate rttomo neurons 6
Wed May 26 16:18:41 2010 cedric 05/26 14:00 PM 16:30 PM f30cryo cryoimaging sections 0
Thu May 27 13:39:37 2010 Spilman 5/27 9:00 AM 2:00 PM standard serviceman none Replace V15 & IGP4
Fri May 28 18:01:56 2010 cedric 05/28 09:00 AM 18:00 PM f30cryo cryoimaging cryosection 0 do not let Ron fix the microscope
the day before you leave the lab. lots of them
1. software stuck on cryocycle the
whole night. IGP1 was at 99 in the
morning. had to go to supervisor
mode with david to restart the
whole vaccum process twice.
2. the upper camera was not cooled
down either. had to reboot the
camera controler to cool it down.
3. tracking ball was set on
joystick and beam moving all other
the place without being able to
stop it.
4. bottom left quadrond (I cannot
remember how to write it) of the
ultracam dead black. impossible to
use the gif until fixed.
in summary: a day in hell...
Wed Jun 2 09:11:09 2010 Chuck Robertson 05/19 9:00 AM 12:30 PM fishione rttomo S. solfataricus 1
Wed Jun 2 09:11:45 2010 Chuck Robertson 05/26 8:30 AM 11:40 AM fishione rttomo S. solfataricus 1
Wed Jun 2 12:39:20 2010 Chuck Robertson 06/02 9:00 AM 12:40 PM fishione rttomo M barkeri 2 On arrival: col. vac: 32,
extraction voltage: 3800. (hv off)
Beam current today: 22.6 nA, twice
as high as one week ago The default rod seems to be a bit
leaky...
Fri Jun 4 17:59:56 2010 Janet Meehl Fox/Winey 6/4/10 10:45 AM 6:00 PM tiltrotate rttomo Tet poc1delete bbs, WTtet bbs 6 Before taking the gain reference,
the upper right quadrant of the
camera image was lighter, both in
the trial and record mode. After
the gain ref was taken, everything
was fine.
Mon Jun 7 12:18:48 2010 morphew 6-7 8 AM 12 AM tiltrotate rttomo aditi neurons 1
Mon Jun 7 15:46:30 2010 Joanna 06/07 12:30 PM 3:40 PM standard training training with Kristin 0
Tue Jun 8 17:38:49 2010 eileen 6/8 8:45 AM 5:30 PM tiltrotate rttomo chlamy flagella 6
Wed Jun 9 12:05:38 2010 eileen 6/9 9 AM 12 PM tiltrotate rttomo chlamy flagella 2
Wed Jun 9 17:33:15 2010 Janet Meehl Fox/Winey 6/9/10 12:30 PM 5:45 PM tiltrotate rttomo WTtet new basal bodies 6
Thu Jun 10 14:47:35 2010 parkkm 6-10 7:30 AM 2:45 PM tiltrotate rttomo hela midbody 4 upper right camera quadrant very
light in comparison
Fri Jun 11 12:00:09 2010 cedric 06/11 8:15 AM 12:00 PM tiltrotate rttomo pml-minus-virus 1
Fri Jun 11 16:47:19 2010 eileen 6/11 12:15 PM 4:45 PM tiltrotate rttomo chlamy flagella 4
Mon Jun 14 16:54:20 2010 Kristin 6/14 9:30 AM 5:30 PM tiltrotate rttomo HeLa 4
Tue Jun 15 16:21:26 2010 eileen 6/15 8:15 AM 4:15 PM 70 rttomo Odde Ase1 delete 8
Thu Jun 17 17:39:45 2010 cedric 06/17 10:00 AM 5:30 PM tiltrotate rttomo polyoma virus pml-- cells 2
Fri Jun 18 16:58:41 2010 cedric 06/18 10:00 AM 17:00 PM tiltrotate rttomo polyoma-pml-- cells 2 jump or shift of the beam around 38
degrees. start the tilt series at
60 and jumps after 21 or 22 images
2 times in a row. I do not
understand why the tracking in
SerialEM didn't account for it.
Mon Jun 21 17:12:08 2010 cedric 06/21 10:00 AM 17:30 PM tiltrotate rttomo polyoma-pml--cells 2
Tue Jun 22 17:30:33 2010 cedric 06/22 10:00 AM 17:30 PM tiltrotate rttomo polyoma-pml--cells 2
Wed Jun 23 13:12:00 2010 Chuck Robertson 06/22 8:10 AM 1:10 PM fishione rttomo M barkeri 2 beam: 6.6nA
Fri Jun 25 12:31:57 2010 morphew 6-25 8:30 AM 12:15 PM tiltrotate rttomo aditi neurons 4
Fri Jun 25 17:59:33 2010 Janet Meehl Fox/Winey 6/25/10 12:40 PM 6:00 PM tiltrotate rttomo WTtet new basal bodies 4 Upper right quadrant looked
different on taking the first
record image before doing the gain
ref. Once the gain ref was taken,
everything looked fine.
Ultracam work done during session
by David M. and Cindi S.
Tue Jun 29 11:49:29 2010 eileen 6/29 8:30 AM 11:45 AM tiltrotate rttomo Odde yeast E data drive making funny sound-
Cindi turned off. Upper right
quadrant funky.
Wed Jun 30 12:19:52 2010 Chuck Robertson 06/30 8:00 AM 12:15 PM fishione rttomo Mbarkeri 2 beam: 8.54 nA right trackball has problems
Wed Jun 30 17:56:14 2010 cedric 06/30 13:30 PM 18:00 PM tiltrotate rtimage polyoma-a31 0
Thu Jul 1 10:24:00 2010 Janet Meehl Fox/Winey 7/1/10 9:15 AM 10:15 AM standard rttomo WTtet 0 Couldn't get rid of the quadrant
lines after taking a gain ref. The
left 1/2 looks ok but the right
upper and lower quadrants are
noticeable as well as a few
horizontal lines in the upper right
quadrant. I didn't start a tilt
series.
Tue Jul 6 13:52:21 2010 Johanna/Neil 07/06 9:00 AM 1:45 PM fishione rttomo trypanosome collar 1 Microscope shut down HT three times
in one hour. Service called.
Thu Jul 8 15:10:36 2010 spilman 7/7 9:00 AM 4:30 PM standard serviceman HT issues & PM 0 HT 50 volt supply replaced, HD
backup, water leak electronics rack
(replaced hose & clamps PM & HT issues
Thu Jul 8 15:12:20 2010 spilman 7/8 8:00 AM 4:00 PM standard serviceman PM 0 PM & HT issues PM & HT issues
Fri Jul 9 18:07:39 2010 Janet Meehl Fox/Winey 7/9/10 12:15 PM 6:20 PM tiltrotate rttomo WTtet assembling basal bodies 6 Camera was still showing
quadrants. David worked some magic
and got a homogenous gain ref.
Sun Jul 11 16:48:39 2010 DNM 7/11 2:00 PM 4:45 PM standard serviceman quadrant correction, setting up
data disks 0 I don't like the bumps in the
quadrant correction curves. Right
now binnedx2 works fine on a
binnedx2 gain reference but not so
well on an unbinned reference
Mon Jul 12 17:59:34 2010 DNM 7/12 11:00 AM 11:45 AM standard serviceman Camera problems 0 Upper right quadrant gain sometimes
varies a few percent
Mon Jul 12 18:00:26 2010 DNM 7/12 12:01 PM 6:00 PM standard development Contemplation and fixing settling
in walkup 0
Tue Jul 13 12:28:47 2010 eileen 7/13 8:50 AM 12:30 PM tiltrotate rtimage Odde yeast
Tue Jul 13 16:55:34 2010 cedric 07/13 13:00 PM 17:00 PM tiltrotate rttomo pml-- 1 upper cam sucks
Wed Jul 14 18:35:56 2010 cedric 07/14 13:00 PM 18:45 PM tiltrotate rttomo A31-accumulation-virus 1 camera sucked again
Thu Jul 15 11:56:39 2010 morphew 7-14 8 AM 11:50 AM tiltrotate rttomo PyV 3
Thu Jul 15 14:05:36 2010 DNM 7/14 11:55 AM 2:05 PM tiltrotate development testing tilt range assessor 0
Fri Jul 16 16:22:56 2010 DNM 7/16 9:30 AM 10:30 AM standard development more tilt range testing
Fri Jul 16 16:23:29 2010 DNM 7/16 10:30 AM 4:25 PM standard serviceman camera video board work
Mon Jul 19 10:57:46 2010 morphew 7-19 9 AM 11 AM tiltrotate rttomo tour to richard easingwood 1
Mon Jul 19 16:24:52 2010 Chuck Robertson 07/19 1:00 PM 4:30 PM fishione rttomo Mbarkeri 2 Beam = 7.85 nA
Tue Jul 20 13:47:42 2010 Cindi/Joey 07/16 9:00 AM 1:45 PM f30cryo cryoimaging Giardia cytos Too humid to work and grids not so
good.
Tue Jul 20 18:37:03 2010 cedric 07/20 2:30 PM 19:00 PM tiltrotate rttomo a31-polyoma 2
Wed Jul 21 18:32:29 2010 cedric 07/21 7:00 AM 6:40 PM tiltrotate rttomo a31-polyoma-accumulation 3
Thu Jul 22 15:16:38 2010 morphew 7-22 8 AM 3:30 PM tiltrotate rttomo melanoma 4
Fri Jul 23 12:51:19 2010 DNM 7/23 9:40 AM 12:55 PM standard serviceman Replacing card in upper camera
controller, rerunning quadrants
Fri Jul 23 18:00:41 2010 cedric 07/23 1:30 PM 6:00 PM tiltrotate rttomo a31-virus-accumulation 2
Mon Jul 26 18:02:39 2010 Cindi/Joey 07/26 9:30 AM 6:00 PM f20cryo cryotomo Giardia Cytos 6 Great day! Needed to reload alignments. Beam
was weird.
Tue Jul 27 12:21:06 2010 Cindi 07/27 8:00 AM 12:20 PM f30cryo cryoimaging Microtubules samples sucked vacuum crash removing rod.
Wed Jul 28 16:46:56 2010 eileen 7/28 11 AM 4:50 PM tiltrotate rttomo DeCamilli endophilin TKO 4
Fri Jul 30 15:13:25 2010 eileen 7/30 8:50 AM 3:15 PM tiltrotate rttomo DeCamilli endophilin control 4
Mon Aug 2 16:44:57 2010 Chuck Robertson 08.02 1:00 PM 4:45 PM fishione rttomo M barkeri 2 beam = 9.52 nA
Tue Aug 3 14:35:59 2010 eileen 8/3 8:40 AM 2:30 PM tiltrotate rttomo DeCamilli endophilin TKO 6
Fri Aug 6 16:30:15 2010 cindi/guobin 08/06 8:30 AM 4:30 PM f30cryo cryotomo polio infected cells 1
Sat Aug 7 16:45:51 2010 Cindi/Guobin 08/07 9:00 AM 5:00 PM f30cryo cryotomo Poliovirus infected Hela cells
Sun Aug 8 20:22:35 2010 Guobin 8/8 9:00 AM 8:00 PM f30cryo cryotomo Poliovirus infected Hela cells The column vacuum read 18 with RT
holder in the column this morning
when I came in. The vacuum read
decreased to 6 in 15 minutes after
RT holder was removed.
Mon Aug 9 15:56:34 2010 Chuck Robertson 08/09 1:00 PM 3:52 PM fishione rtimage Mbarkeri, desiccated 0 Beam = 8.92 nA Vacuum on the rods was very
unpredictable today. Both the
default rod and fischione were
very, very slow to drop below
13... ended my session early
because the vacuum would not go
below 20. The pattern was, start
the twist, about 2/3 around the
vacuum jumps to 35 or greater.
Insert rod, vacuum either stays at
35 ("forever", eg > 15 min) or
drops to ~20 and goes no lower
(last attempt, let it set for 20
minutes, no change.)
Fri Aug 13 13:32:35 2010 Scott Wadewitz 08/10 9:30 AM 4:00 PM standard serviceman Checking out camera/GIF
Fri Aug 13 13:32:42 2010 Scott Wadewitz 08/11 9:30 AM 4:00 PM standard serviceman Checking out camera/GIF
Fri Aug 13 13:32:46 2010 Scott Wadewitz 08/12 9:30 AM 4:00 PM standard serviceman Checking out camera/GIF
Fri Aug 13 13:33:37 2010 Scott Wadewitz 08/13 9:30 AM 4:00 PM standard serviceman Checking out camera/GIF Camera getting sent back for o-ring
service. Small vacuum leak found
1min after air compressor turns
off. Can't find evidence of other
vacuum leak within Gatan system.
Sun Aug 15 16:53:19 2010 DNM 8/15 2:00 PM 5:00 PM 70 development Testing multiple TS 1
Mon Aug 16 17:58:57 2010 DNM 8/16 11:00 AM 6:00 PM 70 development Testing multiple TS 3
Tue Aug 17 12:31:04 2010 spilman 8/16 8:30 AM 9:00 AM standard serviceman test Z axis inoperative from panel,
ordered new
Wed Aug 18 17:00:14 2010 Kirmse 8/18 9:30 AM 5:00 PM f30cryo cryoimaging IFs 0 during LM search on the grid column
vacuum went up to 26 once closed
valves waited for it to come down
afterwards everything was ok,
had some trouble with beam
alignments, reloaded them from file
then
Thu Aug 19 18:41:28 2010 DNM 8/19 10 AM 6:50 PM 70 development multiple TS, cooking and beam
center problems with LD
Fri Aug 20 17:33:45 2010 DNM 8/20 3.00 PM 6:30 PM 70 development testing multiple TS Mouse left button is unreliable
Mon Aug 23 15:46:13 2010 DNM 8/23 10:30 AM 4:50 PM 70 development checking balance shifts in LD and
navigator
Tue Aug 24 16:10:01 2010 cindi 08/24 9:00 AM 4:00 PM f30cryo cryoimaging giardia mbp-gfp immuno column vacuum was at 25 when I
arrived--very rainy day. coldfinger
brought vacuum down quickly. stage
had very jerky movements,
especially when moving large
distances in X and near the edge.
Wed Aug 25 17:39:48 2010 Johanna 08/25 9:00 AM 5:40 PM fishione rttomo trypanosome collar 0 After alpha-wobbler, the stage did
not go back to zero but to 15.
Thu Aug 26 13:08:12 2010 morphew 8-26 8 AM 1 PM tiltrotate rtimage PyV
Thu Aug 26 16:30:46 2010 DNM 8/26 10:45 AM 12:00 AM tiltrotate development recalibrate mag offsets, check out
view area shifting
Thu Aug 26 16:31:49 2010 DNM 8/26 3:00 PM 4:30 PM 70 development Check out rotated low dose axis
Wed Sep 1 15:57:42 2010 morphew 9-1 8 AM 4 PM 70 rttomo PyV 1
Thu Sep 2 14:23:17 2010 morphew 9-2 8 AM 2 PM 70 rttomo PyV 1 col vac at 34 at 8am.; Added LN2,
pumped to 6 after 2 hours
Wed Sep 8 16:47:50 2010 DNM 9/8 2:30 PM 4:45 PM standard development checking out pixel/intensity
matching 0 Gain of cameras seems to be wrong
in properties file. UScan is about
18 not 12, and UCam is 8.1 to
10.4. The latter could be due to
the last card change where one
quadrant had a gain of 80% relative
to the others... but the UScan
change is inexplicable.
Thu Sep 9 17:10:09 2010 Johanna 9/9 09:20 AM 5:20 PM fishione rttomo trypanosome collar 2
Mon Sep 13 15:28:35 2010 Chuck R 9/13 1:00 PM 3:30 PM fishione rtimage E COLI X1411 BEAM = 9.50nA
Vacuum was very nice... very solid.
Tue Sep 14 14:53:28 2010 Johanna 9/14 9:30 AM 3:00 PM fishione rttomo trypansome collar 2 Had a bad quadrant in the same
place as it was before. Only
happened once though.
Tue Sep 14 18:17:47 2010 DNM 9/14 3:45 PM 6:15 PM 70 development testing HQ stage montaging 0
Thu Sep 16 09:56:45 2010 morphew 9-16 8 AM 10 AM tiltrotate rttomo PyV 1-2x2
Thu Sep 16 09:57:28 2010 morphew 9-15 8 AM 4 PM tiltrotate rttomo PyV 3-2x2
Thu Sep 16 15:48:12 2010 Robert Kirmse 9/16 10:30 AM 4:00 PM f30cryo cryoimaging IFs 0 Stage was always giving "Tracking
Error" and moving vigorously: but
doing the LMM nevertheless.
Camera Quadrant was messed up
(controller restart helped a bit)
Crashed Vaccum once because the
program wouldn't accept holder
choice but lock-in timer started
and red light on the stage went
out. Inserting the rod resultet in
vac crash.
Fri Sep 17 13:50:50 2010 DNM/Cindi 09/17 10:00 AM 12:00 AM standard serviceman camera quadrant issues Swapped boards/cables and figured
out the new board is bad. Gatan
shiipping one to arrive on Monday
to replace. The bad board is the
bottom board.
Fri Sep 24 17:35:58 2010 DNM 9/24 10:15 AM 5:30 PM tiltrotate development New GIF distortion images, mag
gradients
0 Tuned GIF 9400-34000, saved GIB
settings
Tue Sep 28 10:25:51 2010 DNM 9/28 9:45 AM 10:30 AM 70 development Mary's mag gradient problem 0 Quadrant syill bad, saw small and
large glitches
Tue Sep 28 17:08:51 2010 cedric 09/28 11:00 AM 5:30 PM tiltrotate rtimage plastic virus stuff 0
Wed Sep 29 15:57:48 2010 morphew 9-29 8 AM 4 PM fishione rttomo giardia ltfs 6
Thu Sep 30 14:01:57 2010 morphew 9-30 8 AM 2 PM fishione rttomo PyV 6
Fri Oct 1 18:34:01 2010 Cindi/David 10/1 9:30 AM 6:30 PM fishione training Grand user trial and training on
multi-TS 3
Tue Oct 5 18:01:29 2010 eileen 10/5 8:45 AM 6:00 PM fishione rttomo auto acquire test series 4 lots of stage track errors.
Navigator software for auto acquire
is great!
Wed Oct 6 15:41:32 2010 morphew 10-6 8 AM 4 PM fishione rttomo PyV 6
Thu Oct 7 15:06:46 2010 Cindi 10/07 11:00 AM 3:00 PM standard serviceman Taking off upper camera Tried to get images of bad quadrant
and couldn't get it to go super
bad. Tried different binnings and
doses. Anyway, stole some images
from other sources. Removed camera
and got it ready for shipping.
Requested shipping box from Gatan.
Returning controller and both extra
video boards.
Thu Oct 14 17:19:44 2010 Spilman 10/11 8:00 AM 6:45 PM standard serviceman Tip change New Tip
Thu Oct 14 17:20:59 2010 Spilman 10/12 8:00 AM 7:00 PM standard serviceman Tip change tip change
Thu Oct 14 17:22:05 2010 Spilman 10/13 7:30 AM 6:15 PM standard serviceman Tip change Tip change
Thu Oct 14 17:23:10 2010 Spilman 10/14 8:00 AM 5:45 PM standard serviceman Tip change Tip change ip
Fri Oct 15 12:31:25 2010 cedric 10/15 10:00 AM 12:30 PM tiltrotate rtimage virus sections scope screwed up. alignments done
with gun lens bug. shutter left in
open state. corner of aperture in
every GIF image.
Fri Oct 15 16:34:12 2010 cindi 10/15 3:30 PM 4:30 PM standard serviceman retune GIF retuned GIF and aperture went away.
It was just way off centered. Did
not cryo-cycle since P15 isn't
reading right. Just let the cold-
finger warm up gradually.
Fri Oct 22 16:56:51 2010 cedric 10/22 9:30 AM 17:00 PM f30cryo cryoimaging section 0
Fri Oct 22 17:01:15 2010 DNM 10/22 3:45 PM 5:00 PM f30cryo development fixing montage problem
Mon Oct 25 11:59:47 2010 Ron Spilman 10/18 8:00 AM 5:00 PM standard serviceman work on compustage work on compustage
Mon Oct 25 11:59:52 2010 Ron Spilman 10/19 8:00 AM 5:00 PM standard serviceman work on compustage work on compustage
Mon Oct 25 11:59:56 2010 Ron Spilman 10/20 8:00 AM 5:00 PM standard serviceman work on compustage work on compustage
Mon Oct 25 12:00:00 2010 Ron Spilman 10/21 8:00 AM 5:00 PM standard serviceman work on compustage work on compustage
Mon Oct 25 16:56:39 2010 DNM 10/25 12:30 PM 5:00 PM tiltrotate development interpolated dark refs and aligning
with rotation
Tue Oct 26 12:12:59 2010 Cindi 10/26 10:30 AM 12:15 PM standard serviceman Install Controller Controller came bad. It cannot read
temperature and goes to -199
quickly, then reads -1. Happens
when no cable is attached, so cable
is good. Sending controller back.
Tue Oct 26 16:44:48 2010 roy 10/26 1 PM 5 PM standard training EELS
Wed Oct 27 16:24:31 2010 roy 10/27 10:30 AM 4:30 PM standard development EFTEM FeO particles
Wed Oct 27 19:39:07 2010 DNM 10/27 4:30 PM 7:40 PM standard serviceman complete waste of time Computer gave a blue-screen crash
for previous user. DM prefs files
were unreadable and it was very
funky, with bad pixel alignments.
Got prefs files from backup disk,
it is OK now. But then the HT went
out. nothing but problems
Thu Oct 28 09:55:31 2010 cindi 10/28 9:30 AM 9:50 AM standard serviceman HT out HT won't go stabily beyond 4kV,
sometimes will spike up to 10kV.
Ron suggests new tank or cable
needed. Will come in this afternoon
to see.
Fri Nov 19 15:39:41 2010 cindi 11/19 9:30 AM 3:30 PM standard serviceman checking fat dewar and column vacuum column vacuum at 6 using Ron's
holder at 9:30. Changed to our
holder and vacuum spiked to 26.
Vacuum took a long time to settle
around 10, but fluctuated a lot
(after adding LN2). Re-inserted
Ron's holder and it spiked to 46
and also took a long time to settle
with LN2 on. Fat dewar looks like
it might last 8 hours in current
configuration. Need to make more
insulation.
Tue Nov 30 17:37:23 2010 DNM 11/30 4:00 PM 5:40 PM standard development a few bugs
Fri Dec 3 10:36:14 2010 moe 12/3 8:30 AM 10:45 AM standard serviceman Align GIF 0
Fri Dec 3 15:04:01 2010 DNM 11/3 1:30 PM 3:00 PM standard development Testing automatic termination on
dim images 4 Right, 4 tilt series of 6 images
each!
Sun Dec 5 10:02:35 2010 DNM 11/5 9:20 AM 10:05 AM standard serviceman Checking gain reference and redoing "Burned" spot now down to 4% dimmer
Mon Dec 6 17:01:19 2010 eileen 12/6 8:30 AM 5:00 PM fishione rttomo spd5(RNAi) 6 Scope worked great! Some
adjustments to exposure time and
target counts needed.
Tue Dec 7 15:00:45 2010 Chuck Robertson 12/07 8:15 AM 3:00 PM fishione rttomo E. coli MC4100 2 Beam = 10.95 nA None!
Wed Dec 8 12:00:08 2010 Cindi 12/8 8:00 AM 12:00 PM fishione rttomo Giardia CPD cytos 2 3X3 montages
Wed Dec 8 16:17:59 2010 Geiss Dec 8 1:00 PM 4:00 PM standard rtimage Carbon mtl 0
Thu Dec 9 12:12:51 2010 Janet Meehl Fox/Winey 12/9/10 8:15 AM 12:15 PM tiltrotate rttomo Tetrahymena poc1delete 4
Thu Dec 9 15:55:21 2010 Cindi 12/9 1:00 PM 4:00 PM standard rtimage Giardia CPD cytoskeletons Had to reset goniometer because I
went to far in the stage. mage
didn't shift between panels of
montage one time only. Bug in
SerialEM that David saw about image
shift limits in montaging. Vacuum
spiked pretty high inserting rod
several times.
Fri Dec 10 16:57:23 2010 DNM 12/10 4:15 PM 5:00 PM 70 serviceman Fixed shutter connections!
Mon Dec 13 15:30:43 2010 Cindi 12/13 11:30 AM 3:30 PM fishione rttomo giardia cytos 6 vacuum was spiking VERY high when
inserting rods (T/R with Mary,
standard, and Fischione)--up to 64.
Sometimes, tilting stage to 60,
waiting a bit, then tilting to 0
brought the vacuum down rapidly.
Both IGPs spiked.
Tue Dec 14 14:49:31 2010 morphew 12-14-10 12:30 AM 3 PM tiltrotate rttomo PyV 2
Wed Dec 15 12:06:15 2010 Janet Meehl Fox/Winey 12/15 8:15 AM 12:00 PM tiltrotate rttomo Tet poc1delete bbs 5 No problems with the column vac.
Wed Dec 15 16:15:04 2010 Roy Geiss 12/15 12:30 PM 4:30 PM standard rtimage FeO - ZnO Image in focus on viewing screen
was not in focus on ULTRACAM Image in focus on viewing screen
was not in focus on ULTRACAM
Thu Dec 16 20:05:34 2010 Johanna 12/16 11:00 AM 8:00 PM fishione rttomo trypanosome collar 8
Mon Dec 20 14:26:44 2010 Cindi 12/20 9:30 AM 2:30 PM fishione rttomo giardia 4 column vacuum at 13 when I got in.
Checked Fischione oring before
using and it was covered with hair.
Checked standard oring and it had a
few hairs. Stage had difficult time
moving in X.
Thu Dec 23 13:49:50 2010 Cindi 12/23 8:00 AM 2:00 PM tiltrotate rttomo Giardia 4 vacuum at 6 when I came in. Vacuum
spiked high inserting both
fischione and T/R holders. Had to
tilt to -60 to get vacuum to drop.
Several times, vacuum spiked into
20s with large stage movements.
Tue Dec 28 10:12:28 2010 Chuckr 12/28 8 AM 10 AM standard rtimage none 0 not usable due to vacuum problems Vacuum on arrival: 34
After 30 min cooling: 34
At -60 degrees: 34
Remove rod drifts to 24
Standard rod has many fiber under
disecction scope... some as long as
8 mm
Replace std rod... vac spikes to 38
Goes to 24 on full insert
Vacuum is erratic... jump 5-10
values between measurements, eg 12
to 22 in successive col vac values.
Not using scope today because of
this.
Wed Dec 29 17:12:51 2010 Janet Meehl Fox/Winey 12/29 9:30 AM 5:30 PM tiltrotate rttomo Tet poc1delete bbs 9 Vacuum was good. I had to tilt
every time to get vacuum to drop
after rod insertion but it always
came down. I was careful when
inserting the rod and didn't have
any spikes over 35. No spikes when
stage moved. Went over o-rings
with a fine-toothed tweezer.
Tue Jan 4 15:19:06 2011 Chuck R 01/04 8:00 AM 3:15 PM fishione rttomo M barkeri 4 Beam = 8.21 nA Rods still fuzzy when they come out
of the scope, but not as bad as
last week.
Col vac spiked to ~28 when
inserting the rods... had to go
very, very slowly... when the rods
were first seated the vacuum was
often 16-20. The magic recipe du
jour was to rotate to +60... this
always brought the col vac back to
6.
Other than twitchy rod insert, the
machine was very stable today.
Wed Jan 5 14:35:50 2011 Chuck Moe 1/5 08:30 AM 14:30 PM standard serviceman stage service cleaned stage orings and realigned
stage. Performed EFTEM alignments.
Thu Jan 6 15:47:30 2011 Cindi 01/06 1:00 PM 3:30 PM standard rtimage Giardia cpd cytos
Fri Jan 7 16:19:26 2011 eileen 1/7 8:15 AM 4:15 PM fishione rttomo c. elegans spd5 9 scope vac and stage GREAT!
Completely fixed.
Mon Jan 10 16:58:07 2011 eileen 1/10 9 AM 5:00 PM fishione rttomo C. elegans spd5 10 O=rings are still a little fuzzy.
be sure to look at them under the
disection scope.
Tue Jan 11 14:46:36 2011 morphew 1-11 9 AM 3 PM f20cryo cryoimaging PyV A31
Wed Jan 12 16:32:32 2011 eileen 1/12 8:45 AM 4:30 PM fishione rttomo De Camilli; WT recovery and Dynamin 6
Thu Jan 13 15:33:15 2011 Cindi 01/13 8:00 AM 1:00 PM standard rtimage Giardia CPD cytos Stage felt a little squirely.
vacuum spiked once to 46 when
inserting rod.
Fri Jan 14 16:28:18 2011 roy 01/14 1:00 PM 4:30 PM standard development HRTEM Apatite
Tue Jan 18 14:37:18 2011 morphew 1/18 12:30 AM 2:30 PM tiltrotate rttomo ltfs/giardia 2
Wed Jan 19 15:04:24 2011 eileen 1/19 8:30 AM 3:00 PM tiltrotate rttomo Tomoko H40 spindles 3 2x3
Thu Jan 20 15:49:32 2011 Cindi 01/20 8:00 AM 3:45 PM tiltrotate rtimage Giardia Sxns Vacuum was at 13 when I logged in.
It wasn't until noon that it got to
6 with holder in.
Fri Jan 21 11:48:39 2011 eileen 1/21 9:30 AM 11:45 AM tiltrotate rttomo Tomoko H40 1 2x3
Fri Jan 21 14:00:09 2011 Cindi 01/20 12:00 PM 2:00 PM tiltrotate rtimage Giardia control samples had it happen twice with a bad
lower left quadrant (much, much
brighter than the others).
Tue Jan 25 11:11:48 2011 Chuckr 01/25 7:45 AM 11:15 AM fishione rtimage Mbarkeri 0 Mag knob sticks/jumps
Tue Jan 25 17:32:49 2011 Johanna/Neil 01/25 1:00 PM 5:30 PM fishione rttomo trypanosome collar 4
Wed Jan 26 09:16:30 2011 cindi 01/26 8:00 AM 9:00 AM standard serviceman vacuum recovery Last night vacuum crashed while
inserting rod. Could not recover.
Told Johanna to go home. This
morning, vacuum still didn't
recover. Tried cycling vacuum. No
help. Removed holder. Vacuum
instantly recovered to 49 and
dropped to 21 within 30sec. Found a
couple of hairs on o-ring. Decided
to replace o-ring. Inserted rod
(burped to 47) and cooled cryo-box.
Vacuum down to 6.
Fri Jan 28 07:39:38 2011 Ron Spilman 01/26 1:30 PM 3:30 PM standard serviceman try to fix compustage leak Ordered new o-rings
Fri Jan 28 07:40:42 2011 Ron Spilman 01/27 8:00 AM 5:30 PM standard serviceman try to fix compustage leak replaced various o-rings, stage still leaking at
high tilt and extreme X. Major burp of IGP
Fri Jan 28 17:08:44 2011 Spilman 1/28 8:00 AM 5:30 PM standard serviceman Service 0 Compustage vacuum leak. Ordered
hemispherical bearing, bearing cup.
oring set, & IGP Scope has not pump below 14 without
houlder or 23 with holder. Was
able to tilt +-79 degrees at
extreme Y cordinace without
leaking.
Mon Jan 31 10:57:23 2011 JohannaCollar 01/27 09:30 AM 11:00 AM fishione rttomo Collar 0 Vaccum broke as I did eucentricity.
Fri Feb 4 19:53:15 2011 spilman 1/31 9:00 AM 5:00 PM standard serviceman service 0 stage vacuum leak
Fri Feb 4 19:53:37 2011 spilman 1/31 9:00 AM 5:00 PM standard serviceman service 0 stage vacuum leak
Fri Feb 4 19:54:20 2011 spilman 1/31 9:00 AM 5:00 PM standard serviceman service 0 stage vacuum leak
Fri Feb 4 19:55:20 2011 spilman 2/1 9:00 AM 5:00 PM standard serviceman service 0 stage vacuum leak
Fri Feb 4 19:56:04 2011 spilman 2/2 9:00 AM 5:00 PM standard serviceman service 0 replace ion getter pump
Fri Feb 4 19:57:26 2011 spilman 2/3 9:00 AM 6:30 PM standard serviceman service 0 stage alignment issue clamping block
Fri Feb 4 19:58:32 2011 spilman 2/3 6:30 AM 9:00 PM standard serviceman service 0 stage alignment issue clamping block
vacuum issues returned
Mon Feb 7 14:17:37 2011 Cindi 02/07 1:45 PM 2:15 PM standard rtimage Giardia CPD Stage moved OK, but did no tilting
Wed Feb 9 12:23:05 2011 spilman 2/7 7:00 AM 1:00 PM standard serviceman stage issues 0 shift in X when tilting ordered parts
Wed Feb 9 12:24:48 2011 spilman 2/9 7:00 AM 1:00 PM standard serviceman stage issues 0 Replaced X donator, but still have
X shift during tilting ordered parts
Thu Feb 10 18:11:19 2011 Johanna 02/10 10:30 AM 18:10 PM fishione rttomo trypanosome collar 6 -first the scope did not tilt more
than to 22 degrees. Ron fixed that
-the lower left quadrant of the
camera showed brighter than the rest
-the vacuum broke as i did the walk
up. Holder is stuck in compustage
Tue Feb 15 16:05:37 2011 spilman 2/10 3:00 PM 5:00 PM standard serviceman stuck rod & vac leak Removed rod and checked for vacuum
leak. Scheduled to install
floating rod on the 11th.
Tue Feb 15 16:07:59 2011 spilman 2/11 8:00 AM 6:00 PM standard serviceman Floating Rod Replacement Disassembled and re-assembled
compustage with new floating rod.
Allowed to pump down over weekend.
Tue Feb 15 16:09:09 2011 spilman 2/14 8:00 AM 5:30 PM standard serviceman Floating Rod Replacement Started stage alignment and vacuum
checking. Vacuum in column was 14
over the weekend. Trouble-shot gun
shift issues.
Tue Feb 15 16:10:04 2011 spilman 2/15 8:00 AM 5:00 PM standard serviceman Floating Rod Replacement Aligned compustage and checked for
vacuum leaks and tilt shift
issues. Job complete.
Wed Feb 16 10:18:07 2011 Cindi 02/16 8:00 AM 10:15 AM tiltrotate serviceman Testing compustage Vacuum at 6. Put in T/R holder,
would not move in X. Tried gonio
reset and had unresolvable pole
touch. Called Ron and reset
compustage screw on clamp. Reset
gonio worked. Fischione holder
works fine with test TS and test
LMM. Put in F30 cryo holder. Does
not move in X. Reset gonio. Ron to
come in morning to fix v-groove.
Wed Feb 16 17:08:19 2011 Johanna 02/16 10:30 AM 05:00 PM fishione rttomo trypanosome collar 8
Thu Feb 17 13:19:03 2011 morphew 2-17 8 AM 11 PM f30cryo cryoimaging liposomes nanogold stage would not translate--service
called
Thu Feb 17 13:58:37 2011 Ron Spilman 02/17 11 AM 12 PM f30cryo serviceman worked on compustage compustage was sticking with
holder. Ron came in and removed the
silver ring on the purple plate.
Did extensive testing. Should be OK
now.
Fri Feb 18 15:25:37 2011 spilman 2/17 12:00 PM 3:00 PM f30cryo serviceman test stage failed on cryo holder removed
ring from front plate
Fri Feb 18 15:27:51 2011 spilman 2/17 10:00 AM 4:00 PM tiltrotate serviceman test stage failed on tily/rotate
adjusted set screws for horse shoe
and set screw under X motor
Mon Feb 21 15:32:18 2011 morphew 2-21 8 AM 3:30 PM f30cryo cryotomo liops 2
Tue Feb 22 09:54:29 2011 cindi 02/22 8:00 AM 10:00 AM tiltrotate rtimage Giardia CPD Yesterday, vacuum at 27 when
arrived. Today, vacuum at 20 when
arrived. Both days: Stage not
translating past -500 in Y. Grid
doesn't seem 'centered'. Made call
to service.
Wed Feb 23 08:29:11 2011 Chuck Moe 2/23 06:30 AM 09:00 AM standard serviceman service Adjusted Y stage movement. Found
bad P1 lens current. Lens checked
and good. Reseated all lens
electronics P1 now working and rotation problem
fixed. Stage movement good
Wed Feb 23 12:43:46 2011 DNM 11/23 10:15 AM 12:45 PM standard serviceman checking stage cal, looking at
quadrant problem
Wed Feb 23 17:16:24 2011 Johanna 2/23 1:00 PM 5:15 PM fishione rttomo tryps flagella 4 Moving sample using the red cross
does not work at low mag. Davod has
called service
Thu Feb 24 17:20:16 2011 eileen 2/24 8:30 AM 5:20 PM fishione rttomo C. elegans montage embryos Thomas MR 2 2x2
Fri Feb 25 17:46:33 2011 Johanna 2/25 10:40 AM 6:00 PM fishione rttomo trypanosome flagellum 3
Fri Feb 25 20:13:32 2011 Chuck Moe 2/22 12:00 PM 6:00 PM standard serviceman compustage worked on compustage and Projector lens 1
issues.
Tue Mar 1 17:43:09 2011 eileen 3/1 8:30 AM 5:30 PM tiltrotate rttomo CDC20 serials 8 Scope working great. Lots of fibers
on o-rings so be sure to check!
Wed Mar 2 16:34:42 2011 Johanna 3/2 10:30 AM 5:00 PM fishione rttomo Trypanosome flagella 4
Thu Mar 3 13:21:13 2011 morphew 3-2 8 AM 1 PM f30cryo cryotomo liposomes 1 extra low contrast, stage not
translating properly
Thu Mar 3 18:27:18 2011 DNM 3/3 2:00 PM 6:30 PM standard development Chasing montage bugs
Fri Mar 4 13:06:42 2011 eileen 3/4 8:30 AM 1:00 PM fishione rttomo wt_K-Stim DeCamilli neurons 6
Fri Mar 4 15:41:06 2011 Cindi 03/04 1:10 PM 3:40 PM fishione rttomo Giardia CPD of MBP-GFP 1 Finally!
Mon Mar 7 19:11:45 2011 Cindi 03/07 11:00 AM 7:15 PM fishione rttomo Giardia CPD of MBP-GFP 5 Stage seemed to lose some ability
on the right-click and drag.
Tue Mar 8 15:38:06 2011 morphew 3/8 11 AM 3:30 PM tiltrotate rttomo u2os ltfs 4
Wed Mar 9 15:10:43 2011 morphew 3-9 8 AM 3 PM tiltrotate rttomo u2os ltfs 5
Wed Mar 9 19:01:54 2011 Kirmse 03/09 4:00 PM 7:00 PM f20cryo cryoimaging IFs 0
Thu Mar 10 17:53:07 2011 Johanna 3/10 10:30 AM 6:00 PM fishione rttomo Chlamydomonas 6
Fri Mar 11 15:06:40 2011 Johanna 3/11 9:00 AM 3:00 PM f20cryo cryotomo trypanosomes 0 F20 cryo holder crashed vacuum
twice on exit.
Sat Mar 12 18:27:04 2011 Johanna 3/12 12:30 PM 6:30 PM fishione rttomo trypanosomes 4
Mon Mar 14 21:55:57 2011 Johanna 3/14 9:30 AM 10:30 PM fishione rttomo trypanosome collar/connector 16
Tue Mar 15 18:55:20 2011 eileen 3/15 8:30 AM 7:00 PM tiltrotate rttomo U2OS augmin RNAi 4 3x2
Wed Mar 16 16:53:06 2011 eileen 3/16 8:45 AM 5:10 PM tiltrotate rttomo CDC20 6 1x2
Thu Mar 17 15:18:11 2011 morphew 3-17 8 AM 3:15 PM tiltrotate rttomo ltfs u2os 6
Fri Mar 18 16:45:50 2011 eileen 3/18 10 AM 4:45 PM tiltrotate rttomo cik1 delete Odde 8
Tue Mar 29 17:16:15 2011 eileen 3/29 8:45 AM 5:15 PM tiltrotate rttomo CDC20 10 seemed ok
Thu Mar 31 12:48:35 2011 Ron 03/21 8:00 AM 5:00 PM standard serviceman Work on Compustage Worked on Compustage
Thu Mar 31 12:48:39 2011 Ron 03/22 8:00 AM 5:00 PM standard serviceman Work on Compustage Worked on Compustage
Thu Mar 31 12:48:44 2011 Ron 03/23 8:00 AM 5:00 PM standard serviceman Work on Compustage Worked on Compustage
Thu Mar 31 12:48:49 2011 Ron 03/24 8:00 AM 5:00 PM standard serviceman Work on Compustage Worked on Compustage
Thu Mar 31 12:48:54 2011 Ron 03/25 8:00 AM 5:00 PM standard serviceman Work on Compustage Worked on Compustage
Thu Mar 31 12:49:03 2011 Ron 03/28 8:00 AM 5:00 PM standard serviceman Work on Compustage Worked on Compustage
Thu Mar 31 12:49:53 2011 Cindi 03/31 6:30 AM 12:00 PM tiltrotate rttomo MBP-GFP Sections 4 Vacuum at 26 when I came in. Found a bug in
SerialEM for using exposure time during tilt-
series acquisition.
Fri Apr 8 16:01:27 2011 Spilman 4/1 1:00 PM 7:00 PM standard serviceman Stage issues
Fri Apr 8 16:01:58 2011 Spilman 4/2 8:00 PM 7:00 PM standard serviceman Stage issues
Fri Apr 8 16:02:35 2011 Spilman 4/5 8:00 PM 7:00 PM standard serviceman Stage issues
Fri Apr 8 16:02:43 2011 Spilman 4/6 8:00 PM 7:00 PM standard serviceman Stage issues
Fri Apr 8 16:02:52 2011 Spilman 4/7 8:00 PM 7:00 PM standard serviceman Stage issues
Fri Apr 8 16:03:19 2011 Spilman 4/8 8:00 PM 7:00 PM standard serviceman Stage issues
Mon Apr 11 15:34:28 2011 Cindi 04/11 8:00 AM 10:00 AM tiltrotate rttomo MBP-GFP 1to50 0 vacuum at 38 when arrived. Removed
holder, dropped to 16. Reinstert
holder to start pre-pump, dropped
to 6. Vacuum crept up to 38 at one
point during routine imaging (with
valves closed!).
Mon Apr 11 15:34:54 2011 Cindi--tour 04/11 10:00 AM 11:00 AM tiltrotate training tour 0
Mon Apr 11 15:35:48 2011 Ron Spilman 04/11 11:00 AM 4:00 PM tiltrotate serviceman work on compustage leak 0 pulled stage, lubricated orings,
adjusted stage. Cindi double-
checked and seems to be working
Tue Apr 12 07:59:32 2011 Cindi 04/12 7:35 AM 8:00 AM standard rtimage shrinkage dataset vacuum at 6
Tue Apr 12 15:20:58 2011 Spilman 11/12 11:40 AM 3:20 PM standard serviceman Vacuum leak stage Replace oring with 6.63 from 6.62mm
Tue Apr 12 17:49:12 2011 eileen 4/12 8:30 AM 5:45 PM tiltrotate rttomo chalmy mitotic 2 vac issues in the morning. Ron
replaced o-rings and is working
now. May be intensity callibration
issues? Used at spot size 1 and it
was ok.
Wed Apr 13 07:18:00 2011 Cindi 04/13 7:00 AM 7:15 AM standard serviceman Checking beam brightness Redid supervisor alignments. Got
beam to 8.10na. Logged in as myself
and loaded new alignments. Got
8.59na. Decided not to reboot. Beam
is just brighter today.
Wed Apr 13 11:33:58 2011 eileen 4/13 8:30 AM 11:30 AM tiltrotate rttomo chlamy mitotics 2 intensity up over 8 at spot 2 now.
Now issues with vac or intensity
today.
Wed Apr 13 19:16:44 2011 Janet Meehl Fox/Winey 4/11 1:00 PM 7:30 PM tiltrotate rttomo WT and poc1del tetrahymena basal
bodies 6 Got an error in serial EM about
beam intensity so I went to spot
size 1 and it worked fine.
Thu Apr 14 15:27:25 2011 kirmse 04/14 9:30 AM 3:30 PM 914 cryotomo IFs 0 really messed up, objective
aperture was way out, couldn't get
a e- dose reading at first, beam
centering is off between images.
might be my alignment file, or the
reloaded service one and everything
has to be redone for my account
Mon Apr 18 16:09:40 2011 eileen 4/15 1:30 PM 5:15 PM tiltrotate rttomo tub4-25degrees 3 1x2
Mon Apr 18 16:10:57 2011 eileen 4/18 8:30 AM 4:10 PM tiltrotate rttomo tub4 25 degrees 3 plus 3 1x2
Tue Apr 19 16:17:38 2011 chuckr 04/19 8:20 AM 4:15 PM fishione rttomo M barkeri 4 beam marginal (7.5 nA)
Vacuum very nice, very solid...
Tue Apr 19 18:21:52 2011 Janet Meehl Fox/Winey 4/15 11:15 AM 1:15 PM tiltrotate rttomo poc1del tetrahymena 2
Thu Apr 21 15:55:11 2011 Cindi 04/21 8:00 AM 4:00 PM tiltrotate rttomo Giardia MBP-GFP ICC Cytos 1 1X2. It took a LONG time to find a
sample. David and I redid the beam
intensity calibrations for spot 2,3
(on right side of crossover). David
redid the crossover calibrations.
Fri Apr 22 12:57:33 2011 Cindi 04/22 7:15 AM 1:00 PM tiltrotate rttomo Giardia Immuno-labeled Cytos 3 Vacuum was at 15 the last 3 days.
Fri Apr 22 18:08:18 2011 Janet Meehl Fox/Winey 4/22 1:00 PM 6:00 PM tiltrotate rttomo WTtetrahymena basal bodies 6
Sat Apr 23 18:35:17 2011 Johanna 4/23 10:00 AM 6:30 PM fishione rttomo trypanosomes old flagellum 2
Mon Apr 25 13:25:15 2011 Cindi/David 04/25 8:00 AM 1:00 PM standard serviceman Working out serialEM calibs alignment seems to be way off. Ron
said to try a reboot. EFTEM is
definitely way off.
Mon Apr 25 19:25:44 2011 Cindi 04/25 1:45 PM 7:30 PM tiltrotate rttomo Kin14b GFP Cytoskeletons 8 Had some serious drift/focus
issues. Redid rotation center and
it seemed to help a little.
Tue Apr 26 16:20:37 2011 Chuck R 04/26 8:30 AM 4:20 PM fishione rttomo M barkeri 4 used c2 = 150u = 19 nA beam none
Tue Apr 26 18:02:28 2011 morphew 4-25 4:15 PM 6:30 PM tiltrotate rttomo ptk
Wed Apr 27 15:45:57 2011 Ron 04/27 8:00 AM 2:00 PM standard serviceman redo alignments redid all alignments (including
EFTEM). New service alignment
called Service042711
Wed Apr 27 15:46:33 2011 Cindi 04/27 3:00 PM 3:30 PM tiltrotate rtimage check sample checked alignments and eftem.
seemed fine.
Thu Apr 28 21:52:06 2011 Matthew West 8:00 AM 5:00 PM tiltrotate rttomo yeast ER Mitochondria 8 Pole touches close the valves--
easily corrected, thanks for the
assistance Cindi!
Thu Apr 28 21:52:52 2011 Matthew West 5:00 PM 10:00 PM tiltrotate rttomo yeast MVBs 5 Odorizzi Time
Fri Apr 29 17:27:20 2011 eileen 4/29 8:30 AM 5:30 PM tiltrotate rttomo tub4 25 degrees 6 HT/Feg shut off- message ODP
Heater. Recycled vacuum and all was
ok. HT/FEG shut off, camera vac shot
up. Column valves closed at the
time and the column vac not
affected. Message: ODP Heater.
Called Ron and he suggested
recycling vacuum. The system
recovered and all was fine the rest
of the day. ?? He is going to order
another ODP heater.
Sun May 1 09:51:32 2011 chuckr 5/1 9:15 AM 9:50 AM fishione rttomo none 0 peoui.exe would not start... hangs
at "Loading control panels".
Mon May 9 16:45:23 2011 eileen 5/9 8:30 AM 4:45 PM tiltrotate rttomo WT yeast 25 degrees 4 1x2 scope worked great!
Tue May 10 15:47:45 2011 Chuck R 05/10 8:30 AM 3:50 PM fishione rttomo M barkeri montage 3 beam was puny for my purposes at
spot 2,c2 = 100 micron. Otherwise
the machine was very stable.
Wed May 11 06:44:16 2011 Spilman 5/3 8:00 AM 5:00 PM standard serviceman test Scope intermintently shuts down
Wed May 11 06:45:23 2011 Spilman 5/3 7:30 AM 12:00 PM standard serviceman test Scope intermintently shuts down
Wed May 11 06:46:31 2011 Spilman 5/5 7:30 AM 12:00 PM standard serviceman test Scope intermintently shuts down Replace faulty relay
Wed May 11 15:24:47 2011 Janet Meehl Fox/Winey 5/11/11 9:00 AM 3:30 PM tiltrotate rttomo WTtet basal bodies 6 Nanoamp reading was very high (over
200) but the beam looked normal.
David checked it out and couldn't
figure out why it was high. I
didn't have any issues.
Fri May 13 17:17:29 2011 eileen 5/13 1:30 PM 5:15 PM tiltrotate rttomo CDC20 4
Fri May 13 17:17:41 2011 eileen 5/13 1:30 PM 5:15 PM tiltrotate rttomo CDC20 4
Fri May 13 17:19:37 2011 spilman 5/13 9 AM 1 PM standard serviceman service 0 Ron came in yesterday (5/12) and
today to fix the screen meter.
Replaced a board and it is now
working.
Mon May 16 17:13:22 2011 eileen 5/16 8:30 AM 5:15 PM tiltrotate rttomo CDC20 7 1x2
Tue May 17 17:11:34 2011 Chuck R 05/17 8:30 AM 5:10 PM fishione rttomo M barkeri 4 spot size 2 beam = 6.13 nA to dim
to use... used spot size 1 beam =
12.6 nA No alignments for spot size 1 below
2000x? Beam appears as once
concentrated spot, controls have no
impact on it.
Wed May 18 16:52:28 2011 Janet Meehl Fox/Winey 5/18/11 9:15 AM 5:00 PM tiltrotate rttomo Tetrahymena WT & poc1Del basal
bodies 7 None!
Thu May 19 13:41:22 2011 morphew 5-19 8:30 AM 1:30 PM tiltrotate rttomo ptk, chromatin 4
Fri May 20 17:04:51 2011 eileen 5/20 8:30 AM 5:00 PM tiltrotate rttomo P.pacificus 5 2x2
Sat May 21 18:05:49 2011 chuck r 05/21 8:55 AM 6:05 PM fishione rttomo M barkeri 4 spot size 1 beam = 12.26 nA none...
Mon May 23 16:36:29 2011 eileen 5/23 8:30 AM 3:30 PM tiltrotate rttomo P.pacificus 2 2x2 Cindi accidentally dumped power
while working with UPS. Called
service and restarted scope from
wall. The ZEM chiller did not come
back online after the reboot, so
lenses aren't working. A ticket was
opened to fix chiller.
Tue May 24 15:05:12 2011 Aztlano Archuleta 05/24 12:30 PM 3:05 PM standard serviceman ZEM Chiller not running 0 Found ZEM Chiller powered off. The
main 6 A fuse was blown. Replaced
and watched it run for a few
minutes. Looks good but would like
the temp stabilize before using the
scope.
Wed May 25 07:44:59 2011 Atzlano 05/24 10:00 AM 2:00 PM standard serviceman work on ZIM chiller replaced fuse on zim chiller.
Wed May 25 07:50:46 2011 Cindi 05/25 7:30 AM 7:45 AM standard serviceman checked beam beam not very bright ~6na at spot
2, 20K.
Wed May 25 14:34:40 2011 morphew 5-25 8 AM 2:30 PM tiltrotate cryotomo chromatin 2
Thu May 26 19:47:12 2011 Matt West 5/26 8:10 AM 6:00 PM tiltrotate rttomo wt yeast ER 12 Voeltz lab; Scope worked superbly
Thu May 26 19:51:32 2011 Matt West 5/26 6:00 PM 7:45 PM tiltrotate rttomo wt MVBs with pGO642 1 I was planning 4 more TS, but had
to end my session early I had problems with the stage map
not recognizing the location on the
section. Worse, the tracking was
lost at high tilt near the center
of the grid for no obvious reason
even after multiple attempts with
different cells and realignments.
I'm stumped...ending my Odorizzi
session early.
Thu May 26 19:56:37 2011 Matt West 5/26 6:00 PM 7:45 PM tiltrotate rttomo wt with pGO642 1 Follow-up to problem: The screw
was not entirely screwed into the
holder. Hopefully no damage was
done to the threads. Likely
explains the short TS of my first
Odorizzi sample.
Sun May 29 08:20:43 2011 Chuck R 5/28 8:55 AM 6:10 PM fishione rttomo M barkeri 4
Tue May 31 08:12:29 2011 Chuck R 5/29 8:15 AM 5:30 PM fishione rttomo M barkeri 4
Wed Jun 1 16:28:01 2011 eileen 6/1 8:30 AM 4:30 PM tiltrotate rttomo augmin U2OS 2 2x3
Thu Jun 2 09:58:28 2011 Maria-Cindi 6/2 8 AM 10 AM standard training training maria is learning
Fri Jun 3 13:09:10 2011 Cindi 06/03 8:00 AM 11:00 AM standard serviceman F30 Down UPS Failed. Took scope to 'line' power to bypass
UPS. Scope comes up on standby, but computer
doesn't work right. Looks like bad video card.
Called service. Aztlano came to look at it.
Fri Jun 3 16:34:01 2011 Aztlano Archuleta 06/03 11:00 AM 4:45 PM standard serviceman Computer would not turn on. Found Video card in the CPU was
bad. Replaced with one from
another site. Ordered one and will
return that one to the other site.
Cold started the FEG and slowly
rasied the HT. All appears to be
normal so back to CU.
Sat Jun 4 16:35:07 2011 Chuck R 06/04 8:15 AM 4:45 PM fishione rttomo M barkeri 4 Used 150um C2 and spot size 2 =
13.18 nA
Mon Jun 6 17:01:38 2011 eileen 6/6 2:00 PM 5:00 PM tiltrotate rttomo tub4 high mag ends 2 screen meter reading is not correct
(<< below zero na). Cindi called
for service.
Tue Jun 7 16:42:57 2011 eileen 6/7 9:00 AM 4:45 PM tiltrotate rttomo tub4/Vogel and augmin U2OS/Tomoko 2 plus 2 2x3
Wed Jun 8 12:48:35 2011 Aztlano Archuleta 06/08 8:45 AM 12:45 PM standard serviceman Exposure Time on Screen Found the wire connecting the large
screen to the exp. read out circuit
was broke. Repaired and checked
exp from large to small screen. It
appears to be working fine. System
is back to CU.
Thu Jun 9 16:23:33 2011 chuck r 06/09 11:00 AM 4:30 PM fishione rttomo M barkeri 2 c2 = 150
spot = 2
beam = 10.5 down almost 3 nA from
previous session with same spog and
same c2
Fri Jun 10 10:28:17 2011 cindi 06/10 7:45 AM 10:30 AM standard serviceman Scope was down when I got in called service and Aztlano walked
me through bringing scope back up.
Cold start after vacuum and desired
states were "ready". brought
extraction volt. to 4250, started
HT at 20 and ramped every 10 to
200, then to 300.
Fri Jun 10 13:07:20 2011 Cindi 06/10 10:45 AM 1:00 PM standard serviceman scope shut down Scope shut down again. Brought up
like last time, only with warm
start.
Fri Jun 10 14:54:53 2011 Aztlano Archuleta 06/10 1:30 PM 2:50 PM standard serviceman Unexplained Powering Off Tool was running fine when I came
in but it turned off twice today.
Checked the relay in mains power,
K1, that was replaced a few weeks
ago, was fine. Checked the +/-
15v, 24v and 5v power supplies.
All were fine. The 75v PS was
reading 76.7v which is good but the
center supply is very loud and is
blowing much hotter air then the
otehr two. Ordered a replacement
75vdc PS PN 5322 695 16042. I left
the back and front panels off or
open to get more air flow. I also
am leaving the curtain open on one
side to get more cooling to the
mains power rack. It can be used
this way for now. Back to CU.
Sat Jun 11 17:42:07 2011 Chuck R 06/11 8:30 AM 6:45 PM fishione rttomo M barkeri 4 C2 = 150 um
spot 2
beam = 12.01nA
Mon Jun 13 13:56:30 2011 Spilman 06/13 8:30 AM 2:00 PM standard serviceman Power supply Wrong power supply ordered. Looked
for Fan, but couldn't find one. put
back in old power supply and will
replace fan tomorrow.
Mon Jun 13 16:52:41 2011 eileen 6/13 2:30 PM 5:00 PM tiltrotate rtimage U2OS mitotics- scanning
Tue Jun 14 16:22:33 2011 spilman 6/14 12:30 PM 4:00 PM standard serviceman test 0 Replace power supply fan
Wed Jun 15 18:00:14 2011 DNM 6/15 4:00 PM 6:00 PM standard development fixing stage movement on reading
montage
Thu Jun 16 13:30:23 2011 morphew 6-16 8 AM 1:30 PM tiltrotate rttomo tryps 2
Fri Jun 17 16:42:30 2011 Janet Meehl Fox/Winey 6/17 9:00 AM 5:45 PM tiltrotate rttomo Tetrahymena poc1delete basal bodies 10
Mon Jun 20 15:01:09 2011 Spilman 6/20 8:30 AM 6:00 PM standard serviceman test 0 PM due Made electronic and vacuum checks
as per PM checklist. Replaced
filter, back up harddrive,
replaced apertures checked for
normal operation
Tue Jun 21 09:22:16 2011 Cindi 06/21 08:45 AM 9:00 AM standard serviceman fixed key on back key on back was loose. Reseated and now peoui
works fine.
Tue Jun 21 17:25:08 2011 Chuck R 06/21 8:15 AM 5:45 PM fishione rttomo M barkeri 4 Beam current (c2 = 150 um),
spot 2: 11.57nA Security key loose prevented peoui
from starting
Wed Jun 22 14:59:37 2011 morphew 6/22 8 AM 3 PM tiltrotate rttomo u2os, ltfs 4
Fri Jun 24 15:45:08 2011 Spilman 6/24 10:30 AM 4:00 PM standard serviceman test 0 Replaced C2 and Objective apertures.
apertures look much better.
Mon Jun 27 10:52:16 2011 Matt West 06/28 10:00 AM 4:45 PM tiltrotate rttomo COS cell ER-Mito Contacts 7 Voeltz lab
Tue Jun 28 16:18:27 2011 Chuck R 06/28 8:15 AM 4:15 PM fishione rttomo M barkeri 4 beam = 15.36 nA spot 2, c2 ap size
not known... c2 position = 3 None... well behaved
Wed Jun 29 20:59:22 2011 Matt West 06/29 8:00 AM 9:00 PM tiltrotate rttomo Plastic COS 14 Had a slow start with a poorly
placed grid in the tilt-rotate (my
error), but once corrected worked
well
Fri Jul 1 17:29:28 2011 Janet Meehl/Winey 7/1 9:30 AM 5:45 PM tiltrotate rttomo WTtet basal bodies 8 Couldn't get full tilt range in the
negative direction, got to about 53-
56 degrees and the rotation was
hindered. But those tilt series
got to the +60 ok. Also, it seems
like the tracking ball that centers
the beam wasn't working toward the
end of my session.
Sat Jul 2 12:16:16 2011 DNM 7/2 10:00 AM 12:15 PM 70 serviceman trying to calibrate mag gradients Beam shift alignment and beam-image
alignment were both very screwed
up. I had to realign these in
order to take montages. Saved
alignments in Mast070211 with this.
Tue Jul 5 11:15:38 2011 DNM 7/5 10:30 AM 11:20 AM 70 serviceman Calibrating mag gradients
Tue Jul 5 17:15:30 2011 eileen 7/5 10:30 AM 5:15 PM tiltrotate rttomo U2OS supermontage setup/tests
Wed Jul 6 17:29:11 2011 eileen 7/6 8:15 AM 5:30 PM tiltrotate rttomo U2OS Anaphase supermontage 4 3x3 (2 serial 1x2 supermontage)
Thu Jul 7 16:56:59 2011 cedric 07/07 8:30 AM 5:00 PM standard rtimage virus 0
Fri Jul 8 17:15:34 2011 eileen 7/8 8:30 AM 5:15 PM tiltrotate rttomo U2OS Anaphase supermontage 1 3x3 plus mapping
Sat Jul 9 14:35:02 2011 chuckr 07/09 8:30 AM 2:30 PM fishione rtimage M barkeri 0 Beam = 13.79 nA
Mon Jul 11 17:12:00 2011 eileen 7/11 8:45 AM 5:10 PM tiltrotate rttomo U2OS supermontage 4 3x3 (2 serial 1x2 supermontage)
Tue Jul 12 15:03:29 2011 Chuckr 07/12 8:45 AM 3:15 PM fishione rtimage M barkeri, Ecoli, Grid mapping 0 Column vacuum very slow to come
below 15...
Fri Jul 15 17:06:26 2011 eileen 7/15 8:30 AM 5:10 PM tiltrotate rttomo basal body dup 3
Mon Jul 18 17:01:13 2011 eileen 7/18 8:30 AM 5:00 PM tiltrotate rttomo chlamy basal bodies 3
Tue Jul 19 11:36:05 2011 chuck r 07/19 8:00 AM 11:30 AM fishione rtimage E. coli Beam = 12.33 nA
Tue Jul 19 18:06:44 2011 eileen 7/19 12 PM 6 PM fishione rttomo chlamy basal bodies 7 plus maps
Wed Jul 20 18:13:57 2011 Kirmse 07/20 9:30 AM 6:00 PM fishione rtimage muscle biopsy 1
Fri Jul 22 15:12:09 2011 R Spilman 7/22 1:30 PM 3:30 PM standard serviceman Zem chiller shut down 0 Checked zem, added water, open
curtain a little, monitored ZEM
temp. Left scope a 200KV over
weekend to alow objective to run
cooler over weekend. Can't say yet
if Zem needs to be replace. See
how it does next few days.
Fri Jul 22 15:18:08 2011 eileen 7/22 8:45 AM 1:00 PM fishione rttomo chlamy mitotics 4 The objective lens went out-
chiller failed (30C). Called Ron
and he turned it off, let it cool
down and it came back.
Mon Jul 25 16:59:55 2011 eileen 7/25 8:45 AM 5:00 PM fishione rttomo chlamy 6
Tue Jul 26 16:10:52 2011 chuckr 07/26 8:40 AM 4:15 PM fishione rttomo M barkeri mapping 3 Even with C2 = 150 um, beam current
is too low: 9.40 nA. Had to go to
spot size 1.
Also... had a column vacuum burp...
col vac went to 99 after std rod
removed, but turbo pump stayed on.
VAC System self corrected within
about 5 minutes... no other issues
with vaccuum in this session.
Wed Jul 27 16:15:37 2011 eileen 7/27 8:30 AM 4:15 PM tiltrotate rttomo LLCPK mts 2 2x2
Fri Jul 29 14:52:36 2011 morphew 7/29 8 AM 3 PM tiltrotate rttomo ptk hec1 4 2x1
Mon Aug 1 15:52:44 2011 eileen 8/1 8:45 AM 3:30 PM tiltrotate rttomo Odde LLCPK 2 2x2
Mon Aug 8 16:52:54 2011 eileen 8/8 8:30 AM 4:50 PM fishione rttomo Augmin RNAi Tomoko 2x3 montage The objective lens chiller failed
again. Ron ordered a new one that
will be installed tomorrow.
Wed Aug 10 15:50:41 2011 morphew 8-10 8 AM 3:45 PM f30cryo cryotomo neurons 4 gif was a trouble-maker
Thu Aug 11 14:54:59 2011 Kirmse 08/11 11:00 AM 3 PM fishione rtimage Myosin 0
Fri Aug 12 16:00:58 2011 eileen 8/13 8:30 AM 4:00 PM fishione rttomo Augmin RNAi Tomoko 3 2x3 montage took 3 gain refs due to quadrant
issues.
Wed Aug 17 16:44:06 2011 eileen 8/17 8:45 AM 4:40 PM fishione rttomo Augmin RNAi Tomoko 2 2x3 montages
Wed Aug 24 13:04:08 2011 Cindi 08/24 7:00 AM 1:00 PM fishione rttomo WT Septin lower left quadrant really bad and
fluctuating. Used GIF. Tuned great,
but the image shifts depending on
mag. Need to do alignment.
Thu Aug 25 08:29:54 2011 Cindi 08/25 7:00 AM 8:30 AM fishione rttomo Septin 6 forgot to put TS number yesterday
Thu Aug 25 13:27:36 2011 Cindi 08/25 8:45 AM 1:30 PM standard serviceman quadrant correction/alignments Did quadrant correction on upper
camera. Redid image alignments for
both cameras. Should stay in
position while changing mag.
Fri Aug 26 17:45:35 2011 Robert 08/29 4:00 PM 6:00 PM fishione rttomo IFs 3 Was just a small test thats why I
used the Ultracam, did the 15 frame
averages for the gain ref, but the
lower left quadrant is really bad,
lines and all
Wed Aug 31 16:08:52 2011 morphew 8/31 8 AM 4 PM f30cryo cryotomo neuron whole mount 3
Tue Sep 6 13:11:22 2011 Cindi/Joey 09/05 8:30 AM 1:00 PM f30cryo cryoimaging Giardia cytos grids super contaminated had a real hard time getting vacuum
to drop after adding holder. Grids
very contaminated. GIF tuning went
very long. Had to re-tune several
times.
Tue Sep 6 15:17:30 2011 Joanna Duncan 09/06 8:30 AM 3:00 PM f30cryo cryoimaging Giarida cytos 0 No problems that you don't already
know about.
Thu Sep 8 10:34:41 2011 DNM 9/7 3:30 PM 5:00 PM standard serviceman trying old camera video card
Thu Sep 8 14:17:51 2011 morphew 9/8 8 AM 2 PM f30cryo cryoimaging neurons
Fri Sep 9 16:12:50 2011 DNM 9/9 8:45 AM 4:15 PM standard serviceman Swapping camera cards, seems to
have fixed it
Mon Sep 12 13:21:38 2011 Joanna Duncan 09/12 8:00 AM 1:17 PM f30cryo cryoimaging Giardia cytos with gold 0 Bad alignment between view and
record. Trouble finding samples as
they were not where they appeared
on the LMM.
Tue Sep 13 11:03:02 2011 morphew 9/13 8 AM 11 AM tiltrotate cryoimaging neurons "sample rod used" won't affirm
Tue Sep 13 17:32:32 2011 eileen 9/13 1 PM 5:30 PM fishione rttomo tub4 S360
tub4-S360A 6
Wed Sep 14 17:31:30 2011 Aztlano Archuleta 09/14 8:15 AM 5:30 PM standard serviceman Vacuum Leak Related to Z Axis
Movement Replaced the hemisherical bearing o-
rings. This solved the vacuum leak
related to Z but caused one with
alpha. Replaced the larger bearing
o-ring with the non-45 degree
seam. This solved all vacuum leaks
related to stage movement but it
now has a leak when the rod is
inserted. Will continue to work on
this tomorrow. Dr. Mastronarde
requested that the stage height be
returned to the height which gives
us ~88% on the objective lens in
focus. This hopefully will solve
the image, beam and aperture shifts
he has been seeing. Obj is
currently at 93% and historically
it was closer to 88%. The schedule
has been changed so FEI service can
have the system until Friday
afternoon.
Aztlano Archuleta 720 236-8159 cell.
Fri Sep 16 12:29:21 2011 Aztlano Archuleta 09/16 8:30 AM 12:30 PM standard serviceman Compustage height adjustment and
vacuum leak Replaced the bearing cup and vacuum
was fine with and with out the
holder. Stage moved to limits with
no vacuum increases.
Set stage height lower in the
column. Now obj. lens current is
90.8% at focus in eucentric
height. Did do alignments but not
EFTEM. Back to CU for further
testing.
Fri Sep 16 15:53:57 2011 Aztlano Archuleta 09/16 2:00 AM 4:00 PM standard serviceman Tilt Axis Adjustment Adjusted tilt axis. It looks good
now. Back to CU.
Fri Sep 16 17:35:42 2011 Kirmse 09/16 4:00 PM 6:00 PM standard rtimage Diffraction experiment 0
Wed Sep 21 12:55:13 2011 Cindi 09/21 7:00 AM 1:00 PM fishione rttomo Septins WTB 4
Thu Sep 22 17:12:14 2011 Spilman-Archeleta 9/22 8:00 AM 5:30 PM standard serviceman alignments Aligned eftem
Fri Sep 23 16:58:47 2011 eileen 9/20 8:30 AM 4:00 PM fishione rttomo Augmin RNAi Tomoko 2 2x3
Fri Sep 23 16:59:24 2011 eileen 9/20 10 AM 5:00 PM fishione rttomo bld2_5 bbs 7
Mon Sep 26 16:55:21 2011 eileen 9/26 10 AM 5 PM fishione rttomo bld2-5 6
Tue Sep 27 17:05:14 2011 eileen 9/27 8:30 AM 5:10 PM tiltrotate rttomo C.elegans metaphase supermontage 1x2 supermontage of 3x3 montages
Wed Sep 28 18:50:30 2011 spilman 9/28 8:40 AM 6:55 PM standard serviceman align 0 ETEM Alignements
Fri Sep 30 10:24:57 2011 Joanna 09/29 8:30 AM 1:00 PM f30cryo cryoimaging Giarid gold controls 0 FEM alignment so far off it was not
usable. :( Digital micrograph
crashed twice.
Fri Sep 30 14:17:16 2011 cindi 09/30 8:00 AM 11:15 AM fishione rttomo 2738B septins 2 punched a hole in the grid when
rotation :(
Sat Oct 1 15:41:21 2011 Johanna/Per 10/01 08:00 AM 03:45 PM f30cryo cryoimaging In vitro microtubules
Sun Oct 2 22:39:17 2011 Johanna/Per 10/02 11:30 AM 10:45 PM f30cryo cryoimaging In vitro MTs LMMs are all off, as are where
record and autofocus is in
comparison to where you aim.
Tue Oct 4 12:07:27 2011 Chuck Moe 10/04 08:30 AM 12:00 PM standard serviceman EFTEM alignment Checked EFTEM alignments and
verified successful GIF tuning in
supervisor and customerservice
workspaces
Wed Oct 5 09:44:18 2011 cindi 10/5 7:00 AM 9:45 AM standard serviceman figuring out why account bad Alignments looked good in
supervisor, mary's account. Bad in
mine. Turns out I was just way out
of focus in EFTEM mode, causing the
beam to be super out of alignment.
I fixed focus and all is fine.
Wed Oct 5 11:23:09 2011 Cindi 10/5 9:45 AM 11:25 AM tiltrotate rttomo 2738B 2
Thu Oct 6 14:03:55 2011 Johanna 9/6 10:00 AM 11:00 AM f30cryo cryoimaging In vitro Mts
Fri Oct 7 12:56:20 2011 Spilman 10/07 11:00 AM 1:00 PM standard serviceman ZEM chiller 0 ZEM chiller failed, reset & adjusted
Fri Oct 7 14:35:54 2011 DNM 10/7 1:15 PM 2:30 PM standard development switching SerialEM to mercurial
Mon Oct 10 14:01:03 2011 Charles Moe 10-10 09:00 AM 2:00 PM standard serviceman Water Flow problem ZEM chiller is not working. Re-
routed hoses for Haskras Chiller to
cool OBJ lens temporarily untill
new ZEM chiller arrives.
Tue Oct 11 18:40:34 2011 Eileen 10/11 8:30 AM 6:40 PM tiltrotate rttomo C.elegans supermontage with Steffi 3 2x3 Stage moved by itselft- Ron
rebooted the comupter and it fixed
the problem.
Wed Oct 12 18:21:27 2011 eileen 10/12 8:30 AM 6:20 PM tiltrotate rttomo C.elegans anaphase supermontage/
Steffi 6 2x3 file transfer very slow
Thu Oct 13 14:39:07 2011 Joanna Duncan 10/13 8:30 AM 2:35 PM f30cryo cryoimaging Giarida cytos 0
Fri Oct 14 17:54:30 2011 DNM 10/14 10:30 AM 6:30 PM standard development Recalibrate IS offsets, beat head
against realign to item 0
Sun Oct 16 17:45:24 2011 DNM 10/16 1:15 PM 5:45 PM 70 development Testing realign to item
Mon Oct 17 16:50:18 2011 eileen 10/17 8:45 AM 4:50 PM fishione rttomo tub4-360A Vogel/Winey 6
Tue Oct 18 13:24:35 2011 Joanna 10/18 9:30 AM 1:21 PM f20cryo cryoimaging Not successful 0
Tue Oct 18 16:32:23 2011 eileen 10/18 2:00 PM 4:30 PM fishione rttomo tub4-S360A Vogel/Winey 4
Wed Oct 19 09:57:36 2011 Spilman 10/19 7:45 AM 10:00 AM standard serviceman none Installed and adjusted ZEM chiller
Wed Oct 19 14:55:44 2011 eileen 10/19 11:00 AM 3:00 PM fishione rttomo tub4S360A Vogel/Winey 4
Thu Oct 20 13:30:09 2011 Joanna Duncan 10/20 8:00 AM 1:00 PM f30cryo cryotomo Tilt series of WT cytos 2
Mon Oct 24 16:39:25 2011 eileen 10/24 1 PM 4:40 PM fishione rttomo DeCamilli Dynamin 1/3 KO K+ 5
Tue Oct 25 12:56:38 2011 Kristin 10/25 9:30 AM 1:00 PM fishione rttomo lens capsule 2
Tue Oct 25 17:27:34 2011 eileen 10/25 1:00 PM 5:30 PM fishione rttomo DeCamilli dynamin1/3ko and wt yeast 4
Wed Oct 26 12:52:36 2011 Cindi 10/26 6:30 AM 12:50 PM f30cryo cryotomo Giardia WT Cytoskeletons 2 Had some drift Power went out hardcore at 7AM, but
scope did fine with UPSs.
Thu Oct 27 13:10:47 2011 Cindi 10/27 6:30 AM 1:06 PM f30cryo cryotomo F30 WT giardia cytos 0 drift really bad at high tilt.
Turns out the aperture is affecting
drift with some sort of charging.
No charging near grid bar, no
charging with standard holder at
high tilt. Unclear what the issue
is. FEI needs to come and redo tilt-
axis offset and z-height.
Fri Oct 28 16:15:23 2011 eileen 10/28 8:30 AM 4:15 PM fishione rttomo tub4-S360A and wt Vogel/Winey 7
Mon Oct 31 16:09:26 2011 Spilman 10/31 8:30 AM 4:30 PM f30cryo serviceman Compustage Adjusted V groove and aligned
stage. Checked stage with single
tilt and F20 cryo holders which
worked fine. Found F30 cryo holder
to be dirty. cleanend holder &
checked for normal operation. cold
& warm.
Tue Nov 1 19:01:44 2011 eileen 11/1 8:30 AM 7:00 PM fishione rttomo tub4-360A Vogel/Winey 14
Thu Nov 3 13:04:44 2011 Joey 11/03 8:30 AM 1:01 PM f20cryo cryotomo Tilt series WT cytos 1 Messed up the vacuum-ooops!
Fri Nov 4 13:19:21 2011 morphew 11-5 8 AM 1 PM tiltrotate rttomo neurons 2-3x1
Mon Nov 7 12:50:36 2011 Cindi 11/07 9:30 AM 12:45 PM 70 rttomo 2738ELG 4
Tue Nov 8 15:42:44 2011 eileen 11/8 8:30 AM 3:40 PM tiltrotate rttomo cardiac myocytes Tom (Kohl lab) 2
Thu Nov 10 15:08:55 2011 Joey 11/10 8:00 AM 3:00 PM f30cryo cryotomo TS WT giardia cytos 2 As mentioned previously.
Thu Nov 10 15:19:54 2011 Joey 11/10 8:00 AM 3:00 PM f30cryo cryotomo cytos 2 During TS, in view before second
image beam was lost. Only about to
find it again in LM. Fixed by
pressing Reset Beam in TUI. Managed
to finish TS.
Fri Nov 11 12:35:04 2011 morphew 11-11 8 AM 12:30 PM f30cryo cryoimaging hela-correlativeLM/EM stage moved to extreme position
when touched the trackbal (or
multifunction knobs). Re-booted
computer, fixed the problem.
Mon Nov 14 13:10:47 2011 Cindi 11/14 6:30 AM 1:15 PM f30cryo cryotomo Giardia WT cytos 4 low mag obj should be ~7.9%
Mon Nov 14 17:11:21 2011 eileen 11/14 1:30 PM 5:10 PM tiltrotate rttomo H30 WT Tomoko 2 2x2
Tue Nov 15 18:07:42 2011 eileen 11/15 3:15 PM 6:05 PM tiltrotate rttomo Tomoko H30 wt 2 2x2
Wed Nov 16 12:53:51 2011 Cindi 11/16 8:40 AM 12:50 PM tiltrotate rttomo Septins 2738ELog 4
Wed Nov 16 15:29:32 2011 morphew 11/16 1:30 PM 3:30 PM f20cryo cryoimaging neurons
Thu Nov 17 16:08:25 2011 Joey 11/17 11:00 AM 4:00 PM f30cryo cryoimaging WT cytos 0
Fri Nov 18 16:48:12 2011 eileen 11/18 8:30 AM 4:45 PM tiltrotate rttomo tub4_S360A 9
Mon Nov 21 14:28:40 2011 morphew 11/21 9 AM 2:30 PM f30cryo cryotomo neuron 3
Tue Nov 22 15:35:42 2011 eileen 11/22 8:30 AM 3:30 PM tiltrotate rttomo Celegans anaphase sec16 b axis 3x1 supermontage of 3x2 montages
Tue Nov 29 18:06:17 2011 eileen 11/29 8:30 AM 6:00 PM tiltrotate rttomo tub4 S360A Vogel/Winey 10
Wed Nov 30 13:28:16 2011 Kristin 11/30 8:30 AM 1:30 PM tiltrotate rttomo lens capsule 2
Thu Dec 1 12:46:22 2011 Cindi 12/01 6:30 AM 1:00 PM tiltrotate rttomo septin correlative work 5
Fri Dec 2 11:39:54 2011 Cindi 12/2 7:00 AM 11:45 AM tiltrotate rttomo correlative septins 5 beam crazy bright (16 na)
Mon Dec 5 12:10:13 2011 Joanna Duncan 12/05 8:30 AM 12:00 PM f30cryo cryoimaging Viewed giardia cytos 0
Tue Dec 6 17:55:00 2011 Matthew West 12-6 9:30 AM 6:00 PM tiltrotate rttomo MIM mutants 8 Odorizzi lab Stage controller is sticky in one
spot
Thu Dec 8 16:12:49 2011 morphew 12/8 8 AM 4 PM f30cryo cryotomo neurons 4
Fri Dec 9 14:24:28 2011 Linda 12/9 8:30 AM 2:30 PM f30cryo training streptomyces cryo My account has a problem with
intensity automatic control. And
teh sample was too thick with
bacteria
Mon Dec 12 15:08:41 2011 morphew 12/12 8 AM 3 PM f20cryo cryoimaging neuron
Tue Dec 13 18:36:59 2011 eileen 12/13 9:00 AM 6:30 PM tiltrotate rttomo tub4-S360A Vogel 6 Big dewar still had nitrogen after
9.5 hours
Wed Dec 14 15:33:21 2011 Spilman 11/14 8:00 AM 4:00 PM standard serviceman test 0 PM due, Made electronic & vac
checks as per PM checklist. Replace
ODP oil, water filter, Vacuum hose,
drain compressor & added oil.
Added water to Haskris chiller,
Checked beam & image for normal
operation.
Thu Dec 15 16:37:59 2011 Spilman 11/15 1:00 PM 5:00 PM standard serviceman test 0 Removed compustage cleaned & lubed
orings. Reinstalled, aligned and
tested. Ordered new set of orings.
Left scope logged on and vacuum
logger on over night. Will return
Friday to check status.
Fri Dec 16 08:00:31 2011 Spilman 12/16 7:15 AM 8:15 AM standard serviceman test 0 Checked vacuum logger (OK) Tilted
sample +&- 79 degrees with Y at +&-
500. Moved stage to multiple
limits. No vacuum leaks found.
Fri Dec 16 19:56:10 2011 Linda Sandblad 12/16 10 AM 8 PM f30cryo cryotomo Streptomyces 5
Mon Dec 19 15:20:49 2011 Cindi 12/19 8:00 AM 3:20 PM f30cryo cryotomo Giardia WT cytoskeletons 7 column had higher vacuum when
shutter was opened on holder. It
took hours to get to 6 from around
20. Compustage might need tweaking.
Optical axis 5um from tilt-axis in
extreme stage.
Tue Dec 20 21:55:39 2011 Linda Sandblad 12/20 10 AM 10 PM f30cryo cryotomo cryo keratin 7
Thu Dec 22 00:56:15 2011 Linda 12/21 12 AM 12 PM f30cryo cryotomo FilP cryo 9
Fri Dec 23 18:01:05 2011 Linda 12/23 10 AM 6 PM f30cryo cryoimaging keratin
Mon Dec 26 20:45:20 2011 Linda 12/26 1 PM 9:00 PM f30cryo cryotomo Keratin 1 Was stucked in Cryo cycle since
friday, restart, OK
Tue Dec 27 17:33:20 2011 eileen 12/27 9:00 AM 5:30 PM tiltrotate rttomo Tetrahymena conjugation 8
Wed Dec 28 15:49:11 2011 Cindi & Garry 12/18 9:00 AM 3:39 PM f20cryo cryotomo Training and giardia cytos 1
Thu Dec 29 18:26:21 2011 Linda 12/92 10 AM 18:30 PM f30cryo cryotomo keratin 1
Fri Dec 30 14:42:22 2011 morphew 12/29 8 AM 2:30 PM f30cryo cryotomo neurons 4
Sat Dec 31 01:41:47 2011 Linda 12/30 4 PM 2 AM f30cryo cryotomo FilP 7
Mon Jan 2 13:42:38 2012 morphew 1/2 9 AM 1:30 PM f30cryo cryoimaging LM/EM tests
Tue Jan 3 16:50:48 2012 eileen 1/3 9:00 AM 4:50 PM tiltrotate rttomo scanning RPE1 mitotics 1
Wed Jan 4 15:26:37 2012 morphew 1-4 8 AM 3:30 PM f30cryo cryotomo neuron 4
Thu Jan 5 14:33:15 2012 morphew 1/5 9 AM 2:30 PM tiltrotate rttomo jon martin neurons 2 2x2 vacuum goes up w/o rod in.
Tue Jan 10 17:09:02 2012 Byung-Ho Kang 01/10 8:00 AM 5:00 PM fishione rttomo Arabidopsis cotyledon 16 I did not sign yesterday (1/9)
time in 9:30, time out 6:00 vacuum failed on 9th Jan when the
compustage move up to 60oC. Turned
off according to Cindi's direction
Thu Jan 12 13:36:23 2012 Spilman 1/11 11:00 AM 5:30 PM standard serviceman yest 0 Replaced compustage orings, bearing
cup & ball as needed. Cleaned &
lubricated. Aligned and checked
for normal operation.
Thu Jan 12 13:37:11 2012 Spilman 1/12 8:00 AM 1:45 PM standard serviceman yest 0 Replaced compustage orings, bearing
cup & ball as needed. Cleaned &
lubricated. Aligned and checked
for normal operation.
Thu Jan 12 15:45:08 2012 morphew 1-12 2 PM 3:45 PM standard rtimage rpe1
Fri Jan 13 16:49:23 2012 Joey 01/13 9:00 AM 4:45 PM f20cryo cryotomo tilt series of WT giardia cytos 4
Tue Jan 17 18:09:31 2012 eileen 1/17 11:00 AM 6:05 PM tiltrotate rttomo Vogel WT yeast 6
Wed Jan 18 16:36:07 2012 Cindi 01/18 8:00 AM 10:00 AM 70 rtimage Giardia supermontage couldn't do a supermontage b/c the
movement in X was bad. Aztlano
worked on it.
Wed Jan 18 16:44:50 2012 Aztlano Archuleta 1/18 10 AM 4:45 PM tiltrotate serviceman X drift Found the X encoder sticking a
little. Cleaned off and verified
it was smooth. Also found the V-
Groove was misaligned. Aligned the
V-Groove but it still needs some
slight adjustments. Will be back
in tomorrow for that adjustment.
Thu Jan 19 11:05:00 2012 Aztlano Archuleta 01/19 9:00 AM 11:00 AM f30cryo serviceman V Groove Adjustment Adjusted V Groove with the beta
tilt/cryo holder. Eucentric height
adjusts great at -74um and has very
little shift at +/- 60 degrees.
When using SerialEM to move the
sample it is with in spec less than or = 500nm
in both directions. Back to CU.
Thu Jan 19 15:01:59 2012 morphew 1-19 12:30 PM 3 PM f20cryo cryotomo neuron 3
Fri Jan 20 17:30:29 2012 eileen 1/20 8:30 AM 5:30 PM tiltrotate rttomo Pacificus anaphase 4 2x2 montage
Mon Jan 23 15:17:32 2012 Kristin 1/23 8:15 AM 3:15 PM tiltrotate rttomo lens capsule 3 stage got completely stuck...
luckily Ron was here to take it
apart and get it moving again
Tue Jan 24 12:24:20 2012 Joey 01/24 9:20 AM 12:20 PM f20cryo cryoimaging Did not get any done 0 Compustage was not functioning
correctly. It was not able to move
the stage properly when I tried to
take a lmm. Cedric helped me take
out the rod and try to reset the
stage position but it did not help.
It seems to be having trouble
finding the centre of the stage and
then moving away from there.
Wed Jan 25 16:39:04 2012 eileen 1/25 9:00 AM 4:30 PM tiltrotate rttomo P.pacificus 4 2x2 montages No stage issues!
Wed Jan 25 16:39:47 2012 eileen 1/25 9:00 AM 4:30 PM tiltrotate rttomo P.pacificus 4 2x2 montages No stage issues!
Thu Jan 26 14:39:49 2012 Joey 01/26 9:15 AM 2:30 PM f30cryo cryoimaging None 0 Lots of problems again today. Stage
is is still not working properly.
Seems to be confused about its
position and unable to move
properly in the X direction. Also
had problem with the GIF and
ultracam. Could not get focuses or
eucentric easily. Could not get the
GIF to refine the zero loss peak. I
am not sure if I should have
completely retuned the GIF to fix
this. Also, some of the settings on
my account are now off from what
they should be from the efforts of
various people to fix it.
Fri Jan 27 14:05:48 2012 morphew 1-27 8 AM 2 PM f20cryo cryotomo neurons 4
Mon Jan 30 16:54:58 2012 eileen 1/30 8:45 PM 5:00 PM fishione rttomo chalmy bbs 6
Tue Jan 31 15:00:47 2012 morphew 1-31 8 AM 3 PM f20cryo cryotomo neurons 4
Wed Feb 1 13:46:03 2012 eileen 2/1 8:30 AM 1:45 PM fishione rttomo chlamy bbs 4
Thu Feb 2 17:37:51 2012 Spilman 2/2 10:45 AM 5:45 PM standard serviceman cal/spheres 0 Went through special alignments not
satisfied with results more time
needed.
Mon Feb 6 15:26:39 2012 morphew 2/6 8 AM 3:30 PM f20cryo cryotomo neuron 2
Tue Feb 7 19:20:50 2012 eileen 2/7 2:30 PM 7:20 PM tiltrotate rttomo Vogel wt 4
Wed Feb 8 07:46:36 2012 Cindi 02/07 7:30 AM 1:30 PM standard serviceman Cryo cycle stuck found cryo-cycle stuck. With advice
from Ron, tried rebooting. Had to
manually turn on IGP pumps after
vacuum cycle started. HT couldn't
come on. Ron came in and worked on
it.
Wed Feb 8 08:44:41 2012 Cindi 02/08 7:30 AM 8:45 AM standard serviceman none Stuck in cryo-cycle. Called Ron:
Close V6, turn off turbo. Reboot
computer, after TEM server starts,
start IGP2,1,4. Get out of manual
mode! Start FEG, enable vacuum on
scope. Start warm cycle. Turn on
HT. Bring up HT and extraction
voltage when warm cycle complete.
Thu Feb 9 13:20:16 2012 Spilman 2/7 3:00 PM 4:00 PM standard serviceman none 0 Restarted pumping system after cryo
cycle failed to complete
Thu Feb 9 13:22:34 2012 Spilman 2/9 8:00 AM 9:30 AM standard serviceman none 0 Found P15 gage failed to read
correct vacuum causing cryocycle
failure. Tapped on gage to knock
off whisker/dirt and scope worked
fine.
Fri Feb 10 17:52:09 2012 Joey 02/10 9:15 AM 5:45 PM f20cryo cryotomo wt CYTOS 6
Mon Feb 13 17:01:36 2012 eileen 2/8 8:30 AM 4:00 PM tiltrotate rttomo Vogel wt yeast 8
Mon Feb 13 17:01:53 2012 eileen 2/8 8:30 AM 5:00 PM tiltrotate rttomo Vogel wt yeast 9
Mon Feb 13 17:02:09 2012 eileen 2/13 8:30 AM 5:00 PM tiltrotate rttomo Vogel wt yeast 9
Tue Feb 14 08:23:24 2012 Cindi 02/14 8:00 AM 8:20 AM standard serviceman stuck in cryo-cycle tapped P15 guage and the cryo-cycle
finished. guage needs replacing.
Tue Feb 14 14:17:20 2012 Spilman 2/14 8:00 AM 2:30 PM standard serviceman gold calibration 0 Took head of new p15 gage and
installed electonics of old gage to
make p15 work. Completed special
alignments.
Wed Feb 15 16:35:49 2012 Joey 02/15 9:15 AM 4:31 PM f20cryo cryotomo WT cytos 3
Thu Feb 16 10:11:30 2012 morphew 2/16 8 AM 10 PM tiltrotate cryotomo rpe1 1
Thu Feb 16 15:31:25 2012 Joey 02/16 10:10 AM 3:30 PM f20cryo cryotomo TS WT cytos 3
Fri Feb 17 13:59:09 2012 morphew 2-17 8 AM 2 PM tiltrotate rttomo rpe1 1
Fri Feb 17 15:15:30 2012 cindi 02/17 2:45 PM 3:15 PM standard serviceman PVP pump failure Vacuum crashed during cryo-cycle.
Restarted vacuum and had buffer
tank error. Called Ron and got
column under vacuum. Looks like
buffer tank pump has failed and can
no longer pull vacuum.
Mon Feb 20 14:43:55 2012 Aztlano Archuleta 2/20 10:30 AM 3:00 PM standard serviceman Projection PVP Vacuum Error Replaced the PVP and still had high
vac ~46 log/ 1 torr. Found the
rubber hose to the buffer tank was
cracked and when moved the pressure
will spike up and you could hear
the leak. Replaced but still had
high leak of 1 torr/min. Blanked
off V1 and still had high
pressure. Ordering V1.
Wed Feb 22 11:09:28 2012 Service 02/21 8:00 AM 5:00 PM standard serviceman scope down bad V1 V1 needs replacement. Aztlano accidentally
ordered the wrong part (used FEI V1, when he
should have used Edwards V1).
Thu Feb 23 15:15:37 2012 Aztlano Archuleta 2/23 8:30 AM 3:30 PM standard serviceman Vacuum Problems Replaced V1 and the ODP dipstick o-
rings. Pumped the system down and
checked the beam. Looks good so
back to CU.
Thu Feb 23 18:01:40 2012 DNM 2/24 5:30 PM 6:00 PM standard development looking at rotation on GIF 220 x
for handedness issue Jensen lab
Fri Feb 24 12:06:39 2012 Joey 02/24 9:15 AM 12:01 PM f30cryo cryotomo Cryo 0 I let a little air into the column
and crashed the vacuum, faily minor
and it was able to recover quickly.
Had a problem getting the rod to go
in the last bit when loading the
cryo rod.
Mon Feb 27 16:53:38 2012 Cindi 02/27 6:45 AM 4:48 PM f30cryo cryotomo WT cytos 9 Stage seemed to be far out of
alignment when up at high tilt at -
60. Had to take it there manually
and then realign frequently during
TS. This was improved by starting
the ts at +60 instead.
Tue Feb 28 17:02:28 2012 eileen 1/28 9 AM 4:30 PM tiltrotate rttomo Tomoko Augmin U2OS 3 2x3 montage
Tue Feb 28 17:03:28 2012 David/Eileen 1/28 4:30 AM 5:00 PM standard rttomo alignments 0
Wed Feb 29 14:31:00 2012 morphew 2/29 7:45 AM 2:30 PM f30cryo cryotomo neurons 0
Wed Feb 29 17:57:35 2012 DNM 2/29 2:30 PM 6:00 PM standard serviceman Trying to work with ill-fated
aperture alignment, checking out
GIF camera
Thu Mar 1 12:36:08 2012 Aztlano Archuleta 3/01 08:30 AM 1:00 PM standard serviceman Drift in (-) Alpha Adjusted V-Groove. Drift has been
improved. Please check and let me
know if it needs additional
adjustments.
Fri Mar 2 17:20:17 2012 eileen 3/2 8:45 AM 5:15 PM tiltrotate rttomo Tomoko/Goshima augmin RNAi 3 2x3 montage
Mon Mar 5 15:24:40 2012 morphew 3/5 7:30 AM 3:20 PM f30cryo cryoimaging neuron
Tue Mar 6 16:45:08 2012 eileen 3/6 8:45 AM 4:45 PM tiltrotate rttomo C.elegans supermontage 1 2x3 and 1x3 supermontage of 2x3 montages
Wed Mar 7 16:23:17 2012 cindi 03/07 7:30 AM 1:30 PM f20cryo cryotomo giarida cytos 2 found F20 cryo holder pin slightly
bent. Tested a -4um and -6um tilt-
series to see if ultracam ok (still
unclear). GIF drifting consistenly
to minus side (about -7eV over 45
min).
Thu Mar 8 15:19:13 2012 morphew 3/8 8 AM 3:15 PM fishione rttomo ltfs giardia 4
Fri Mar 9 13:13:53 2012 morphew 3/9 8 AM 1 PM fishione rttomo ltfs giardia 4
Fri Mar 9 18:00:39 2012 eileen 3/9 1:30 PM 6:00 PM tiltrotate rttomo Tomoko normal control 1 3x3 montage and grid scans
Tue Mar 13 17:08:42 2012 eileen 3/13 8:45 AM 5:00 PM tiltrotate rttomo Tomoko wt- H51 serials 3 3x3 montage
Wed Mar 14 15:28:09 2012 eileen 3/14 8:30 AM 3:30 PM tiltrotate rttomo Celegans supermontage 3 2x3 beam seemed dim at spot 2 (~4ish)
Thu Mar 15 13:11:20 2012 morphew 3/15 8 AM 1 PM tiltrotate rttomo ltfs chlamy 4
Thu Mar 15 16:43:31 2012 DNM 3/15 1:00 PM 4:45 PM 70 serviceman mostly futile GIF testing
Fri Mar 16 17:58:41 2012 eileen 3/16 8:45 AM 6:00 PM tiltrotate rttomo Celegans anaphase supermontage 6 2x3 montages
Mon Mar 19 16:19:22 2012 eileen 3/19 8:30 AM 4:15 PM tiltrotate rttomo Celegans anaphase supermontage 3 2x3 montage
Mon Mar 19 19:53:42 2012 DNM 3/19 4:30 PM 7:50 PM standard development testing autoalign changes and nav
correction for shrinkage in realign
Tue Mar 20 16:41:55 2012 eileen 3/20 8:30 AM 4:40 PM fishione rttomo DeCamilli WT_K_1min 8 Scope was giving stage end of range
messages, but was in the middle
when I logged on. Stage got stuck
when doing eucentricity. Chuck
reanabled the stage but it gave the
message again when inserting rod.
David said this happened to him
last night. The scope worked fine
and I only got the stage message
one time after stage was enabled.
Wed Mar 21 15:57:35 2012 morphew 3/21 8 AM 4 PM tiltrotate rttomo rpe1 5
Thu Mar 22 18:05:26 2012 Janet Meehl Fox/Winey 3/22 12:40 PM 6:10 PM tiltrotate rttomo poc1Delete tetrahymena basal bodies 6
Fri Mar 23 11:11:23 2012 eileen 3/23 8:30 AM 9 AM standard rtimage no work done- zem chiller out again 0 Zem chiller is out again. Service
called.
Tue Mar 27 17:30:05 2012 Aztlano Archuleta 03/27 9:30 AM 5:30 PM standard serviceman Zepher Zem 300 to Hasker Replacement Replaced the ZEM with a new Haskers
R050 chiller. Ran into a few
problems with flow and blocked
lines. Those were resolved but now
the PVP will not pump down.
Getting a spare and gauges to
verify if the pump is ok or an
internal leak. System is still
down.
Wed Mar 28 17:09:36 2012 Aztlano Archuleta 03/28 9:00 AM 5:00 PM standard serviceman Haskers Install Replaced the ODP O-Rings and
restarted TEM Vac. All is looking
fine there. Spent the rest of the
day pump purging the Haskers to get
rid of air in the water lines. It
is currently running with vacuum.
Will check tomorrow and finish the
Haskers ZEM swap. System is still
down.
Thu Mar 29 10:27:30 2012 Aztlano Archuleta 03/29 9:00 AM 10:30 AM standard serviceman ZEM To Haskers Replacement Objective lens water still has
bubbles so repumping system and
continuing to purge air out of the
water. Will check back later.
System is still down.
Thu Mar 29 18:05:09 2012 Aztlano Archuleta 3/29 5:30 PM 6:00 PM standard serviceman Haskers R050 Pumped the reservoir again with a
little more air coming out. Will
leave overnight and check
tomorrow. System is down.
Fri Mar 30 14:53:54 2012 Aztlano Archuleta 3/30 9:00 AM 3:00 PM standard serviceman Haskers R050 Spoke with Haskers and it sounds
like we have multiple leaks in the
water tank. This was most likely
caused in shipping. Haskers is
sending me a replacement tank.
System is down.
Tue Apr 3 10:41:02 2012 Service 04/02 8:00 AM 5:00 PM standard serviceman Scope down for bad chiller Still down. Waiting for water tank on chiller.
Wed Apr 4 10:13:35 2012 Archuleta-Spilman 4/3 1:00 PM 4:00 PM standard serviceman none Resealled fittings of Haskriss
chiller vacuum valve. and pumped
over night.
Wed Apr 4 10:15:33 2012 Archuleta-Spilman 4/3 8:30 PM 11:00 AM standard serviceman none Switched on objective lens supply
checked beam & lens currents.
Adjusted wate flow. Checked for
normal operation. Prepare ZEM for
return.
Wed Apr 4 17:22:45 2012 eileen 4/4 11:00 AM 5:20 PM tiltrotate rttomo Chlamy anaphase 4 1x2 montage
Thu Apr 5 14:55:57 2012 morphew 4-5 8 AM 3 PM f30cryo cryotomo neurons 3
Fri Apr 6 16:30:27 2012 eileen 4/6 8:45 AM 4:30 PM fishione rttomo DeCamilli WT K 8
Mon Apr 9 10:56:06 2012 cindi 04/09 8:15 AM 10:45 AM fishione rttomo Giardia in HM20 2 Looked at gain ref of Ultracam. Oil
spots seem to be mostly gone.
Tue Apr 10 14:18:43 2012 Spilman 4/10 8:00 AM 2:20 PM standard serviceman none Made electronic & vacuum checks,
backed harddrive, saved align
files, Tecnai configuration files,
and fingerprint files. Replaced
vacuum hoses, and checked scope for
normal operation.
Wed Apr 11 14:56:53 2012 morphew 4/11 8 AM 3 PM f30cryo cryotomo neurons 5
Fri Apr 13 10:42:03 2012 Cindi 04/13 8:00 AM 10:30 AM f30cryo cryoimaging delta-giardin gfp cytos crashed vacuum removing F30 holder
second time. Also, holder couldn't
get below 13.
Fri Apr 13 15:30:07 2012 cindi 04/13 10:15 AM 3:30 PM tiltrotate rttomo giardia sections 5 sample looks like crap :(
Tue Apr 17 13:30:00 2012 Cindi 04/17 7:30 AM 1:30 PM f30cryo serviceman testing GIF
Tue Apr 17 17:28:39 2012 cedric 04/17 4:00 PM 5:30 PM f30cryo cryoimaging nothing 0
Mon Apr 23 18:03:49 2012 DNM 4/23 10:15 AM 6:00 PM 70 development testing realign to item, trying
special alignment
Tue Apr 24 16:24:04 2012 eileen 4/24 1:00 PM 4:20 PM fishione rttomo Odde LLCPK1 scans and tilt series 1 2x2
Tue Apr 24 21:35:18 2012 DNM 4/24 5:30 PM 9:30 PM 70 serviceman Special alignments - success!
Wed Apr 25 13:03:44 2012 Maria Pagratis 04/25 8:00 AM 1:00 PM 70 training Lense capsule 1
Wed Apr 25 16:33:47 2012 eileen 4/25 1:30 PM 4:30 PM fishione rttomo Odde Mts 2 2x2
Thu Apr 26 17:01:51 2012 Joey 04/26 9:15 AM 4:55 PM f30cryo cryotomo WT cytos 5 GIF would not stay aligned, at one
point I had to realign the ZLP
about every two minutes. Also, a
lot of problems taking tilt series.
I had to stop two as it could no
longer track and would move so far
off imaging area that I could not
realign it and continue. This
occurred suddenly within a single
loop. Not sure what was causing
this. Also seemed to have a lot of
trouble keeping the beam centered
by the end of the session.
Mon Apr 30 22:29:18 2012 DNM 4/30 10:00 AM 10:30 PM 70 development Recalibrate for special alignment,
debug realign to item
Tue May 1 18:30:48 2012 eileen 5/1 8:45 AM 6:30 PM tiltrotate rttomo Tomoko/Ghoshima augmin RNAi 3 3x3 montage
Wed May 2 14:49:30 2012 Cindi 05/02 7:45 AM 2:45 PM f30cryo cryotomo vitreous sxns giardia wt 2 view and preview aren't lined up.
Wed May 2 18:31:37 2012 DNM 5/2 4:15 PM 6:30 PM 70 development realign to item maybe last bug
Thu May 3 16:54:57 2012 Joey 05/03 9.00 AM 4.50 PM f30cryo cryotomo WT cytos 6 No problems with GIF today stage position shown on screen with
crosshairs was a bit off from
actual stage positions
Fri May 4 12:54:50 2012 eileen 5/4 8:30 AM 1:00 PM tiltrotate rttomo Tomoko/Ghoshima augmin RNAi 1 3x3 montage and grid scans
Mon May 7 15:01:32 2012 eileen 5/7 8:45 AM 3:00 PM tiltrotate rttomo Tomoko/Ghoshima augmin RNAi 2 3x3 montage
Tue May 8 15:29:53 2012 Maria Pagratis 05/08 9 AM 3:30 PM tiltrotate rttomo Dr. Chris Goulbourne 4
Mon May 14 18:02:32 2012 Kirmse 05/14 9:30 AM 6:00 PM f20cryo cryoimaging IF 0
Tue May 15 17:52:53 2012 eileen 5/15 9:00 AM 6:00 PM fishione rttomo Candida wt and scans 6
Wed May 16 16:08:22 2012 eileen 5/16 8:45 AM 4:00 PM tiltrotate rttomo Tomoko/Ghoshima augmin RNAi 2 3x3 montage
Thu May 17 15:22:41 2012 Kirmse 05/16 9:30 AM 3:30 PM f30cryo cryoimaging IF 0
Fri May 18 16:06:52 2012 cindi 05/18 9:00 AM 4:00 PM f30cryo cryotomo delta giardin-gfp cytos 5 used special alignments with 30um
aperture. It was awesome! also
increased image shift limit to 2
um. BUT stage needs some
adjustment. It's very wonky during
tilting
Mon May 21 15:20:37 2012 Maria Pagratis 5/21/12 9 AM 3 PM tiltrotate rttomo liposomes 5
Tue May 22 16:44:32 2012 eileen 5/22 8:45 AM 4:40 PM tiltrotate rttomo RPE1 supermontage 2 3x3 montage trouble with realign to item
Wed May 23 13:17:40 2012 morphew 5/23 8 AM 1 PM tiltrotate rttomo chromatin 4
Thu May 24 13:09:23 2012 Spilman 5/24 8:00 AM 1:30 PM f30cryo serviceman Test 1 Compustage alignments and
adjustments for use with heavy
holders.
Tue May 29 18:28:56 2012 DNM 5/29 9:20 AM 6:30 PM tiltrotate development Realign to item bugs with Eileen's
sample
Wed May 30 15:44:40 2012 pagratis 5/31 9 AM 3:30 PM tiltrotate rttomo goulbourne
Thu May 31 15:44:38 2012 Cindi 05/31 8:30 AM 4:00 PM f30cryo cryotomo Giardia delta-gfp cytos 3 lots of issues tilting. probably
need a floating rod. new alignments
and SEM calibrations great!
Fri Jun 1 13:23:04 2012 DNM 6/1 10:30 AM 1:30 PM 70 development Non-centered trimming in realign to
item
Tue Jun 5 13:38:11 2012 eileen 5/4 8:45 AM 12:00 AM tiltrotate rttomo supermontage with overnight 4 2x3
Tue Jun 5 13:39:42 2012 eileen 6/5 12:00 AM 1:30 PM tiltrotate rttomo supermontage overnight acquisition 2 3x2
Tue Jun 5 16:16:45 2012 DNM 6/5 2:00 PM 4:15 PM tiltrotate development Nailed weird realign failure on
second tilt series
Tue Jun 5 17:26:21 2012 eileen 6/5 4:15 PM 5:30 PM tiltrotate rttomo RPE1 mitotic test series 1
Wed Jun 6 08:51:50 2012 Cindi 06/06 8:30 AM 8:45 AM standard serviceman move alignment files moved alignment files and rebooted
computer
Wed Jun 6 13:51:12 2012 Spilman 6/6/ 11:00 AM 4:00 PM standard serviceman none 9 Replace 5 volt supply
Wed Jun 6 19:22:42 2012 Cindi 06/06 2:00 AM 7:15 PM tiltrotate rttomo Giardia sections 1 Tested plasmon imaging on 350 nm
sections.
Thu Jun 7 15:18:35 2012 DNM 6/7 9:00 AM 3:20 PM standard training going over special alignments with
Cedric
Fri Jun 8 15:27:28 2012 eileen 6/8 8:30 AM 4:30 PM tiltrotate rttomo Tomoko/Ghoshima U2OS augmin wt 2 3x3
Mon Jun 11 15:48:43 2012 eileen 6/10 8:45 AM 3:45 PM tiltrotate rttomo Tomoko/Ghoshima WT U2OS 2 3x3 montage
Tue Jun 12 16:13:41 2012 pagratis 6/12 9:00 AM 4:00 PM tiltrotate rttomo goulbourne 2
Wed Jun 13 13:32:01 2012 Cindi 06/13 7:45 AM 1:30 PM 70 rttomo Giardia gamma-matrigel 2
Wed Jun 13 16:11:21 2012 pagratis 6/20 2:30 AM 4:15 PM tiltrotate rttomo Goulbourne 1
Thu Jun 14 14:27:20 2012 Joey 06/14 9:15 AM 2:22 PM f30cryo cryoimaging None :( 0 Got the cryo rod in second time
fine, think I have figured out what
I am doing wrong
Thu Jun 14 16:53:40 2012 DNM 6/14 3:00 PM 5:00 PM 70 serviceman getting mag gradient files
Fri Jun 15 16:59:19 2012 eileen 6/15 8:45 AM 5:00 PM tiltrotate rttomo Tomoko/Ghoshima augmin RNai 2 3x3 montage
Mon Jun 18 17:20:19 2012 eileen 6/18 8:30 AM 5:15 PM fishione rttomo Ghoshima/Tomoko U2OS augmin RNAi 6 2x3 montages
Wed Jun 20 17:21:18 2012 eileen 6/20 1:30 PM 5:30 PM tiltrotate rttomo Basrai spc98 4 + 2 1x2 montage
Thu Jun 21 15:30:32 2012 pagratis 6/21 9 AM 4 PM tiltrotate rttomo goulbourne 4
Tue Jun 26 17:30:48 2012 eileen 6/22 8:45 AM 6:30 PM tiltrotate rttomo Basrai/Choy SPC98 6 (4 1x2 and 2 1x3)
Tue Jun 26 17:31:57 2012 eileen 6/26 8:45 AM 5:30 PM tiltrotate rttomo Basrai/Choy WT 9 (6 1x2 and 3 1x3 montage)
Thu Jun 28 15:35:39 2012 Joey 06/28 9:15 AM 3:23 PM f20cryo cryotomo image WT cytos 3 Some issue with beam not staying
centred for the trial images. Did
not cause a serious problem.
Mon Jul 2 14:21:18 2012 cindi 07/02 8:00 AM 2:15 PM f20cryo cryoimaging giardia cytoskeletons crashed vacuum 2X. Once while
putting holder in. Once while
taking holder out.
Tue Jul 3 18:35:16 2012 eileen 7/2 9:15 AM 6:30 PM tiltrotate rttomo Celegans metaphase supermontage 2 3x3 montage
Thu Jul 5 13:24:41 2012 Joey 07/05 9:00 AM 1:18 PM f20cryo cryotomo WT cytos 1 Drift measured during autofocus
does not correspond to drift
observed during record images in
ts, especially bad at high tilt.
Some instability in the vacuum,
especially when tilting.
Fri Jul 6 19:09:18 2012 Cindi 07/06 8:30 AM 7:00 PM tiltrotate rttomo gamma-matrigel 3 3X4 montages
Mon Jul 9 13:52:29 2012 Spilman 7/9 10:00 AM 2:00 PM standard serviceman service 0 leaky pnuematic solinoid for V3
replaced
Mon Jul 9 16:50:16 2012 morphew 7/9 2 PM 4:45 PM tiltrotate rttomo Hec1 2
Tue Jul 10 18:13:07 2012 eileen 7/10 8:45 AM 6:10 PM tiltrotate rttomo C.elegans anaphase supermontage 6 2x3 montages
Wed Jul 11 15:14:02 2012 pagratis 7/11 9:00 AM 3:00 PM tiltrotate rttomo boulbourne 4
Fri Jul 13 09:36:03 2012 Spilman 7/12 9:00 AM 4:40 PM standard serviceman test 0 Repair vacuum leak in compustage
Fri Jul 13 19:11:14 2012 Cindi 07/13 9:00 AM 7:00 PM tiltrotate rttomo giardia gamma 3 3 4X3 montages found a few bugs in serialEM with
David.
Mon Jul 16 12:23:48 2012 pagratis 7/16 9:00 AM 12:00 PM tiltrotate rttomo goulbourne 3
Tue Jul 17 18:14:20 2012 eileen 7/17 8:45 AM 6:15 PM tiltrotate rttomo Vogel/Kristy tub4_360A 8
Wed Jul 18 15:43:44 2012 eileen 7/18 8:45 AM 3:45 PM tiltrotate rttomo Vogel/Kristy WT 4
Fri Jul 20 09:16:48 2012 cindi 07/19 12:00 PM 12:00 AM tiltrotate rttomo gamma-matrigel 3 4X4 montages had a com error that required a
reset of the goniometer
Fri Jul 20 09:17:11 2012 cindi 07/20 12:00 AM 10:00 AM tiltrotate rttomo gamma-matrigel 3 4X4 montages worked fine!
Fri Jul 20 18:26:28 2012 eileen 7/20 11:30 AM 6:30 PM tiltrotate rttomo Vogel/Krysty wt yeast 6
Mon Jul 23 18:10:06 2012 eileen 7/23 8:30 AM 6:10 PM tiltrotate rttomo RPE1 anaphase supermontage dual axis 1x2 supermontage of 3x3 montages
Wed Jul 25 14:56:08 2012 morphew 7-25 8 AM 3 PM tiltrotate rttomo hec1 4
Thu Jul 26 15:26:52 2012 cindi/karen davies 07/26 8:00 AM 3:30 PM tiltrotate training training training of Karen Davies of MPI for
serialEM
Fri Jul 27 15:32:17 2012 Cindi/Karen 07/27 8:00 AM 3:30 PM tiltrotate training training serialEM training Stage very wonky. Called service.
Need new float rod, but it's on
backorder.
Mon Jul 30 14:58:50 2012 Kristin 07/30 8:00 AM 3:00 PM fishione rttomo e. coli 2
Tue Jul 31 16:54:55 2012 eileen 7/31 11:00 AM 5:00 PM fishione rtimage section survey and montage 0
Wed Aug 1 17:12:18 2012 eileen 8/1 8:45 AM 5:10 PM tiltrotate rttomo RPE1 metaphase 3x3 montage 2 3x3 montage The second data disk died and no
data could be written to e drive.
David disconnected the second drive
and data can be written to the
first drive now.
Thu Aug 2 14:12:08 2012 supervisor 08/02 2:00 PM 2:15 PM standard serviceman Putting scope on standby Scope will be on standby until the
morning of Aug 13 when Ron brings
it back up. Robert will watch the
IGP pumps to be sure they are
working.
Mon Aug 13 10:20:01 2012 Spilman 8/13 8:00 AM 10:30 AM standard serviceman none 0 Brought scope up from standby,
Restarted feg (cold start) Checked
beam & image.
Tue Aug 14 16:58:15 2012 eileen 8/14 9:00 AM 5:00 PM tiltrotate rttomo RPE1 metaphase 4
Fri Aug 17 17:02:55 2012 eileen 8/17 10:30 AM 5:00 PM tiltrotate rttomo RPE1 metaphase scans and montage 1 3x3 montage and grid scans
Mon Aug 20 15:28:55 2012 eileen 8/20 8:45 AM 3:25 PM tiltrotate rttomo RPE1 metaphase 1 3x3 vac crash during second tilt series
acquisition. Camera was also at
room temp. Cindi increased water
flow and temp back to normal now.
Thu Aug 30 13:42:21 2012 Spilman 8/21 9:00 AM 5:00 PM standard serviceman service 0 Install, align compustage floating
rod. monitor vacuum over multiple
days 8/21/2012 thru 8/30/2012.
untill call complete.
Thu Aug 30 13:42:41 2012 Spilman 8/22 9:00 AM 5:00 PM standard serviceman service 0 Install, align compustage floating
rod. monitor vacuum over multiple
days 8/21/2012 thru 8/30/2012.
untill call complete.
Thu Aug 30 13:42:51 2012 Spilman 8/23 9:00 AM 5:00 PM standard serviceman service 0 Install, align compustage floating
rod. monitor vacuum over multiple
days 8/21/2012 thru 8/30/2012.
untill call complete.
Thu Aug 30 13:43:11 2012 Spilman 8/24 9:00 AM 5:00 PM standard serviceman service 0 Install, align compustage floating
rod. monitor vacuum over multiple
days 8/21/2012 thru 8/30/2012.
untill call complete.
Thu Aug 30 13:43:46 2012 Spilman 8/27 1:00 PM 5:00 PM standard serviceman service 0 Install, align compustage floating
rod. monitor vacuum over multiple
days 8/21/2012 thru 8/30/2012.
untill call complete.
Thu Aug 30 13:44:11 2012 Spilman 8/28 8:00 PM 5:00 PM standard serviceman service 0 Install, align compustage floating
rod. monitor vacuum over multiple
days 8/21/2012 thru 8/30/2012.
untill call complete.
Thu Aug 30 13:44:22 2012 Spilman 8/29 8:00 PM 5:00 PM standard serviceman service 0 Install, align compustage floating
rod. monitor vacuum over multiple
days 8/21/2012 thru 8/30/2012.
untill call complete.
Thu Aug 30 13:44:35 2012 Spilman 8/30 8:00 PM 5:00 PM standard serviceman service 0 Install, align compustage floating
rod. monitor vacuum over multiple
days 8/21/2012 thru 8/30/2012.
untill call complete.
Thu Sep 6 13:50:00 2012 spilman 12/5 8:00 AM 6:30 PM tiltrotate serviceman test Adjusted compustage for normal
operation. Found heavy wear on
cryo holder alowing excessive
movement when tilting. Dr
Masternarde ran multiple maps in
serial em. Scope available for use.
Fri Sep 7 15:19:07 2012 eileen 9/7 9:00 AM 3:15 PM tiltrotate rttomo RPE1 metaphase section 7 and baking 2 3x3 montage
Mon Sep 10 17:28:06 2012 eileen 9/10 8:30 AM 5:30 PM tiltrotate rttomo RPE1 metaphase 4 3x3 montage
Tue Sep 11 14:01:03 2012 cindi 09/11 9:00 AM 10:00 AM 70 rttomo mouse heart 0 crashed vacuum with +/-70 holder.
can't use in this scope because the
compustage wasn't calibrated for
it. Vacuum had to be reset, so
switched to F20
Wed Sep 12 15:40:29 2012 Cindi/Chris Gilbourne 09/12 9:00 AM 4:00 PM f30cryo rttomo heart wt 5
Thu Sep 13 12:04:57 2012 Cindi/Chris Gilbourne 09/13 8:00 AM 10:30 AM f30cryo rttomo heart wt sections 3
Fri Sep 14 14:20:56 2012 cindi/minghua 09/14 9:00 AM 2:15 PM f30cryo training training staved pombe crashed vacuum removing cryo-holder
Mon Sep 17 15:49:19 2012 Cindi 09/17 1:40 PM 3:40 PM standard rtimage giardia sections
Tue Sep 18 14:43:09 2012 eileen 9/18 8:45 AM 2:30 PM tiltrotate rttomo RPE1 metaphase 2 3x3 montage
Thu Sep 20 14:18:56 2012 Cindi 09/19 9:00 AM 2:30 PM tiltrotate training Making training videos 1
Thu Sep 20 14:19:10 2012 Cindi 09/20 9:00 AM 2:15 PM tiltrotate training Making training videos 4
Fri Sep 21 15:43:50 2012 Cindi 09/21 8:30 AM 3:45 PM f30cryo training making serialem videos 1 4th grid is the charm!
Tue Sep 25 08:47:28 2012 Cindi 09/24 8:30 AM 4:00 PM tiltrotate training SerialEM demo vidoes lost internet/network connectivity
Tue Sep 25 08:47:50 2012 Cindi 09/25 8:30 AM 8:45 AM tiltrotate training SerialEM demo vidoes cannot connect to bio3d
Wed Sep 26 17:29:01 2012 Janet Meehl Fox/Winey 9/26 1:00 PM 5:10 PM tiltrotate rttomo poc1Del tetrahymena basal bodies 4 When I pushed the eucentric focus
button during the alignment, it was
really off.
Fri Oct 5 15:48:23 2012 Cindi 10/05 8:45 AM 3:45 PM tiltrotate rttomo giardia 2 3X3 montages
Fri Oct 5 17:42:31 2012 DNM 10/5 4:15 PM 5:45 PM standard development testing backlash adjust in Nav
Mon Oct 8 13:02:17 2012 DNM 10/8 10:45 AM 1:00 PM standard development backlash, realign changes
Wed Oct 10 15:30:16 2012 Cindi 10/10 9:00 AM 3:30 PM tiltrotate rttomo Giardia 2 2X3 montages
Thu Oct 11 18:12:37 2012 Matt West 10/11 10 AM 6:15 PM fishione rttomo Cos cell wt 8
Wed Oct 17 13:45:24 2012 Cindi/Jared Polis 10/17 1:00 PM 2:00 PM tiltrotate rttomo giardia tour with jared polis
Tue Oct 23 17:08:33 2012 spilman 10/23 8:00 AM 5:30 PM standard serviceman none 0 PM. Backup harddrive, change PVP
oil, Replace compress air hoses,
clean regulators, install
condensation vent valve. Replace
water filter.
Wed Oct 31 17:11:50 2012 DNM 10/27 11:00 AM 11:00 PM standard development nav testing Was continuously logged in until
10/31, mostly working remotely
Wed Oct 31 17:13:09 2012 DNM 10/31 5:00 AM 5:00 PM standard development nav testing Just a wild estimate of how much
time I was actually on scope since
10/27
Tue Nov 6 18:10:20 2012 eileen 11/5 12 PM 6:10 PM fishione rttomo Murray lab/Natalie WT 6
Thu Nov 8 17:41:15 2012 Matthew West 11/8 9:15 AM 6:00 PM fishione rttomo Cos WT MVBs 12
Fri Nov 9 10:33:07 2012 Gordana 11/9 9:30 AM 10:30 AM standard rtimage EELS data analysis
Tue Nov 13 18:20:54 2012 eileen 11/13/12 11:30 AM 6:20 PM fishione rttomo Natalie 276_ndc10 yeast 4
Wed Nov 14 18:25:29 2012 eileen 11/14 8:15 AM 6:30 PM fishione rttomo Natalie ndc10 and wt 16
Thu Nov 15 12:26:20 2012 Gordana Dukovic 11/15 9:45 AM 12:25 PM standard rtimage EELS data analysis with
DigitalMicrograph
Thu Nov 15 17:04:13 2012 cynthia 11/15 1:00 PM 5:00 PM tiltrotate rttomo human heart chem fixed 2 dual axis
Fri Nov 16 15:55:12 2012 cynthia 11:16 9:15 AM 5:00 PM tiltrotate rttomo human heart 3.5 dual axis
Sat Nov 17 15:25:36 2012 DNM 11/17 1:30 PM 3:30 PM standard development checking realign with view offset
Mon Nov 19 16:15:36 2012 cynthia 11/19 1:30 PM 4:15 PM tiltrotate rtimage human heart
Tue Nov 20 13:10:24 2012 Cindi 11/20 8:30 AM 1:00 PM f30cryo cryotomo bpv 4 tests for John Heumann
Wed Nov 21 14:14:26 2012 cynthia 11/21 10:00 AM 2:15 PM tiltrotate rttomo human heart 1
Mon Nov 26 14:49:28 2012 DNM 11/26/12 10:30 PM 2:50 PM standard serviceman redo ultracam quadrant correction
Tue Nov 27 11:05:00 2012 Cindi 11/27 9:00 AM 11:00 AM f30cryo cryoimaging BPV sample not so good vacuum spiked to 61 when inserting
holder
Tue Nov 27 11:05:02 2012 Cindi 11/27 9:00 AM 11:00 AM f30cryo cryoimaging BPV sample not so good vacuum spiked to 61 when inserting
holder
Wed Nov 28 16:43:47 2012 eileen 11/28 8:30 AM 4:45 PM fishione rttomo Murray/Natalie yeast 173_1.8 6
Mon Dec 3 16:41:41 2012 eileen 12/3 9:00 AM 4:40 PM fishione rttomo Murray/Natalie 293_3sk 6
Wed Dec 5 17:51:17 2012 eileen 12/5 10 AM 5:50 PM fishione rttomo Murray/Natalie ndc10 12
Thu Dec 6 15:43:53 2012 Cindi 12/06 9:00 AM 3:45 PM standard development trying to figure out pixel sizes scope was at 10 when logged on
Fri Dec 7 15:39:28 2012 eileen 12/7 9:45 AM 3:40 PM fishione rttomo Murray/Natalie ndc10 9
Tue Dec 11 17:18:30 2012 eileen 12/11 9:30 AM 5:20 PM fishione rttomo Murray/Natalie ndc10_3sk 11
Tue Dec 18 15:25:35 2012 Kristin 12/18 8:30 AM 3:30 PM standard rttomo M. barkeri and E. coli 14
Wed Dec 19 15:35:12 2012 Cindi 12/19 8:30 AM 3:30 PM tiltrotate rtimage crappy salivary gland samples
Thu Dec 20 17:21:28 2012 Janet Meehl Fox/Winey 12/20 12:15 PM 5:20 PM tiltrotate rttomo Tetrahymena basal bodies 4 Serial EM froze with 3 degrees left
in a tilt series, David M. checked
it out. Data was not lost.
Thu Jan 3 15:36:18 2013 Cindi 01/03 10:00 AM 3:30 PM tiltrotate rtimage giardia 0
Mon Jan 7 16:19:52 2013 Cindi and Ackerson Lab 0/07 1:00 PM 4:20 PM f30cryo cryoimaging Bacteria
Wed Jan 9 18:15:26 2013 eileen 1/9 8:45 AM 6:15 PM fishione rttomo Moore 282_8 8
Tue Jan 15 15:58:49 2013 Cindi 01/15 11:15 AM 4:00 PM standard rtimage bactera +/- selenium tried some EFTEM and EELS. It is
NOT clear that there is Se in the
+Se cells. But there are densities
not present in control.
Wed Jan 16 15:43:20 2013 Cindi/Cynthia 01/16 2:45 PM 3:45 PM standard rtimage salivary glands of fly
Fri Jan 18 18:11:10 2013 eileen 1/18 8:45 AM 6:10 PM tiltrotate rttomo stain tests and Moore 282_2 9
Mon Jan 21 15:58:26 2013 DNM 1/21 3:40 PM 4:00 PM standard development Looking at low-dose pictures before
getting data elsewhere
Tue Jan 22 17:59:36 2013 eileen 1/22 9:00 AM 6:00 PM tiltrotate rttomo Murray/Natalie 293_3sk 6 2x1 montages
Wed Jan 23 17:00:47 2013 eileen 1/23 9:00 AM 5:00 PM tiltrotate rttomo testing unstained sections/ SF9 5 ts plus focus series crashed vacuum- hair on o-ring! vac
recovered fine.
Fri Jan 25 15:18:26 2013 Joey 01/25 9:30 AM 3:15 PM f30cryo cryoimaging Tried to find microvesicles 0 Crashed vacuum - ooopsee! It
recovered very quickly though. Problems with objective apature. I
used the first 30 um apature and
found that it was constantly
drifting off centre. I had to
recentre it about 20 times.
Mon Jan 28 16:43:54 2013 eileen 1/28 9:00 AM 4:45 PM tiltrotate rttomo Patrick Willey/ Candida 3
Tue Jan 29 16:58:44 2013 eileen 1/29 9:00 AM 4:50 PM tiltrotate rttomo Patrick Willey Candida 6
Fri Feb 1 18:13:16 2013 eileen 2/1 9:00 AM 6:15 PM tiltrotate rttomo Murray/Natalie 3sk, Celegans
supermontage mapping 3
Mon Feb 4 16:55:07 2013 eileen 2/4 9:00 AM 4:55 PM tiltrotate rttomo Celegans supermontage 1x3 supermontage of 3x3 montage
Tue Feb 5 19:42:25 2013 eileen 2/5 8:30 AM 7:40 PM tiltrotate rttomo C.elegans anaphase supermontage 6 3x3 montage
Wed Feb 6 10:34:16 2013 DNM 2/6 9:20 AM 10;40 AM standard development image pairs from ultracam
Wed Feb 6 16:47:59 2013 eileen 2/6 10:30 AM 4:50 PM tiltrotate rttomo C.elegans supermontage (1x3 of 3x3) 3 3x3 montages
Fri Feb 8 17:03:11 2013 eileen 2/8 9:00 AM 5:00 PM tiltrotate rttomo C.elegans anaphase supermontage
(1x3 of 3x3) 3 3x3 montages
Mon Feb 11 14:58:09 2013 eileen 2/11 8:45 AM 3:00 PM tiltrotate rttomo C.elegans supermontage T0265_31
(1x3 super of 3x3 montages) 3 3x3 montage
Tue Feb 12 20:20:48 2013 Matt West 2/12 10:30 AM 8:15 PM tiltrotate rttomo Cos mutants 8 Thanks to Eileen I was able to
salvage a scope day after the f-20
camera failed.
Wed Feb 13 09:59:21 2013 Cindi 02/13 8:45 AM 10:00 AM standard rtimage epon FS samples looked like crap :(
Wed Feb 13 16:38:54 2013 eileen 2/12 2:30 PM 4:40 PM tiltrotate rtimage Scanning Drosophila embryo sections 0
Tue Feb 19 15:57:16 2013 cindi 02/19 2:00 PM 4:00 PM standard rtimage Giardia on intestines beam very dim when I logged in,
came up nice with alignments
Wed Feb 20 18:08:19 2013 Cindi 02/20 8:30 AM 6:15 PM standard rtimage giardia intestines
Fri Feb 22 15:57:58 2013 Minghua Liu 02/22 9:10 AM 4:00 PM f30cryo cryoimaging Pombe
Mon Feb 25 17:04:29 2013 eileen 2/25 8:25 AM 5:00 PM tiltrotate rttomo mtth yeast 7 camera error
Tue Feb 26 16:53:52 2013 eileen 2/26 8:45 AM 5:00 PM tiltrotate rttomo MTTH yeast Murray/Natalie 3sk yeast 8
Wed Feb 27 17:08:31 2013 eileen 2/27 11:00 AM 5:10 PM tiltrotate rttomo murray/natalie ndc10 yeast 4 vac went up to 20 when tilting (-
20). cleaned fibers off o-ring and
put new grease. vac was fine the
rest of the day.
Thu Feb 28 11:27:23 2013 Kristin 2/28 8:30 AM 11:30 AM standard rttomo didn't get past alignments 0 Digital Micrograph not recognizing
camera (detailed e-mail sent to
Cindi and Eileen)
Fri Mar 1 15:20:00 2013 Cindi 03/01 9:00 AM 3:20 PM f30cryo cryoimaging BPV, sample too thick DM took 16 times to work. Crashed
vacuum inserting cryo holder the
first time.
Mon Mar 4 14:45:17 2013 DNM 3/4 11:00 AM 2:45 PM standard serviceman Trying to get ultracam to work,
pairs on upper camera
Tue Mar 5 16:32:21 2013 DNM 3/5 10:0 AM 4:30 PM standard development testing dose rates and counts
Fri Mar 8 18:30:48 2013 eileen 3/8 9:00 AM 6:30 PM tiltrotate rttomo Chlamy uni1 6
Mon Mar 11 17:14:54 2013 eileen 3/11 1:00 PM 5:15 PM tiltrotate rttomo Chlamy uni1 8
Tue Mar 12 15:26:18 2013 eileen 3/12 9:00 AM 3:30 PM tiltrotate rttomo Chlamy uni1 5 vac issues (23 at tilt 20)with both
holders. Cleaned o-rings, and it
resolved. no vac issues at all
during tilt series acquisition and
none at all with eucentricity.
Wed Mar 13 18:31:39 2013 Kristin 3/13 8:30 AM 6:30 PM tiltrotate rttomo E. coli 2
Thu Mar 14 15:37:15 2013 Spilman 3/14 8:00 AM 3:45 PM standard serviceman stage 9 Lube stage orings to eliminate leak
during tilt. align stage. adjust
switch for sensing the holders.
Thu Mar 14 15:37:33 2013 Spilman 3/14 8:00 AM 3:45 PM standard serviceman stage 9 Lube stage orings to eliminate leak
during tilt. align stage. adjust
switch for sensing the holders.
Tue Mar 19 18:10:39 2013 eileen 3/9 8:45 AM 6:10 PM 70 rttomo pombe and Drosophila 6 the microscope recognized the
holder on the first insertion, but
then did not unless the turbo was
off and Cindi used her magic touch!
Wed Mar 20 16:48:31 2013 eileen 3/20 9:30 AM 4:50 PM tiltrotate rtimage pombe and Drosophilia supermontage
mapping 2
Thu Mar 21 15:25:23 2013 Cindi 03/21 8:30 AM 3:30 PM f30cryo cryotomo BPV 2
Fri Mar 22 17:12:49 2013 eileen 3/22 2:30 PM 5:10 PM standard rtimage Drosophila scans 0
Mon Mar 25 16:52:34 2013 eileen 3/25 8:45 AM 4:50 PM tiltrotate rttomo Candida 102 6
Mon Apr 1 13:51:51 2013 Cindi 04/01 8:30 AM 2:00 PM tiltrotate rttomo Giardia 2 alpha wobbler stopped when pressing
z-axis button. Reset goniometer,
worked fine after.
Tue Apr 2 14:45:50 2013 Cindi 04/02 9:00 AM 2:45 PM tiltrotate rttomo giardia for fib 4
Wed Apr 3 12:46:02 2013 cynthia 04/02/13 9:00 AM 12:45 PM f30cryo cryotomo bpv got one grid in then we had two
crashes trying to get the second
grid in got one grid in then we had two
crashes trying to get the second
grid in. We are baking out the F30
rod as there was a alot od frost on
the rod itself and the dewar was
cold.
Fri Apr 5 17:34:28 2013 eileen 4/5 9:00 AM 5:30 PM fishione rttomo pombe 2738 3 1x2
Mon Apr 8 10:48:14 2013 Cindi 04/08 10:30 AM 10:45 AM standard rtimage MTs not polymerized
Thu Apr 11 16:40:03 2013 Cindi 04:11 8:30 AM 4:30 PM f20cryo cryoimaging BPV 0 The f30 rod crashed, Cindi will
replace O-ring. The F20 rod worked
OK even after the crash!
Fri Apr 19 16:36:23 2013 Cindi 04/19 8:30 AM 4:30 PM tiltrotate rttomo neurons for FIB 6
Mon Apr 22 15:40:16 2013 Cindi 04/22 8:30 AM 2:30 PM f30cryo cryoimaging BPV sample concentration way too low
Mon Apr 22 15:40:41 2013 Cindi 04/22 2:30 PM 3:45 PM tiltrotate rttomo giardia, testing auto acquire TS 1
Fri Apr 26 15:47:58 2013 Joey 04/26 9:30 AM 3:45 PM f30cryo cryotomo Mth tagged cytos 1
Tue Apr 30 11:51:53 2013 Cindi 04/30 9:30 AM 12:00 PM f30cryo cryoimaging giardia with 17053-mth crappy sample
Thu May 2 14:49:15 2013 Joanna 05/02 8:15 AM 2:45 PM f30cryo cryoimaging none 0 Had problems with the vacuum.
Crashed with F30 rod, then had
trouble with F20 rod. Airlock not
counting down, instead constant
pumping and could not get rod in.
Later, (3 hours) Mary was about to
insert warm cryorod twice and
everything seemed normal.
Fri May 3 11:30:49 2013 Cindi 05/03 9:30 AM 11:30 AM standard serviceman testing vacuum when inserting
holders Tried 3X with F30 cryo holder, 2X
with T/R holder, 3X with F20 cryo
holder. Took some time to come down
after inserting holders, but it
always worked. Also, changed o-ring
on F20 cryo holder.
Mon May 6 15:39:10 2013 DNM 5/6/13 11:00 AM 3:40 PM 70 development Realign to item with huge view
defocus offset
Fri May 10 16:51:54 2013 cynthia 05/10/13 2:00 PM 5:00 PM standard rtimage k4m sections of DES_MTH When I fisrt started the scope
colum vacuum was 30. Removed rod
and cleaned of O ring there were
about 8 threads/hairs! And once the
rod was in the vacuum was back down
again.
Fri May 17 13:13:20 2013 DNM 5/17/13 11 AM 1:15 PM tiltrotate development testing stability of rotating rod
with cable tethered to stage 1 Rotation stopped working - seems to
be the cable
Tue May 21 12:30:52 2013 eileen 5/22 9:15 AM 12:30 PM fishione rttomo candida 102 1
Wed May 22 16:12:33 2013 eileen 5/22 8:45 AM 4:15 PM fishione rttomo Candida 102 anaphase 4
Tue May 28 17:31:08 2013 eileen 5/28 1:00 PM 5:30 PM fishione rttomo Odde Candida 4
Wed May 29 16:56:46 2013 eileen 5/29 8:45 AM 5:00 PM fishione rttomo Odde Candida kip1del 5
Fri May 31 15:23:47 2013 eileen 5/31 9:00 AM 3:30 PM fishione rttomo Odde Candida 12711 5
Mon Jun 3 16:33:26 2013 morphew 6/3 9 AM 4:30 PM f30cryo cryotomo BPV_focus series 4
Tue Jun 4 17:59:41 2013 eileen 6/4 8:45 AM 6:00 PM fishione rttomo Candida strain 102 7 The stage was not always moving to
the right place when dragging in
SerialEM.
Wed Jun 5 16:55:00 2013 eileen 6/5 8:45 AM 5:00 PM fishione rttomo Candida strains 102 and 12711 10
Thu Jun 6 15:36:27 2013 morphew 6/6 1 PM 3:30 PM fishione rtimage tet-MTH
Fri Jun 7 13:42:27 2013 eileen 5/7 10 AM 1:30 PM fishione rttomo Candida 12711 3
Thu Jun 13 17:17:13 2013 Joey 06/13 9:30 AM 5:15 PM tiltrotate rttomo Montage of cyto 17
Mon Jun 17 17:27:40 2013 eileen 6/17 12:00 PM 5:30 PM fishione rttomo Candida 111 3
Tue Jun 18 17:44:18 2013 eileen 6/18 9 AM 5:45 PM fishione rttomo Candida 111 and 12377 5
Wed Jun 19 17:05:44 2013 eileen 6/19 9:00 AM 5:10 PM fishione rttomo Odde Candida 27377 7
Fri Jun 21 13:10:41 2013 morphew 6-21 8:45 AM 1 PM fishione rttomo tetrehymena MTH 2
Mon Jun 24 17:06:41 2013 eileen 6/24 9:45 AM 5:00 PM fishione rttomo Tetrahymena K4M 3MTH 4
Tue Jun 25 18:25:04 2013 eileen 6/25 9:00 AM 6:30 PM fishione rttomo SPC42_MTH freeze sub resins 6
Wed Jun 26 15:45:58 2013 morphew 6-26 10 AM 3:45 PM fishione rtimage mth
Thu Jun 27 16:16:04 2013 c page 6/27 10 AM 4:15 PM tiltrotate rtimage prasad rotovirus 0
Fri Jun 28 17:48:16 2013 cynthia 06/28 10:00 AM 5:30 PM fishione rttomo Prasad rotavirus epon 6
Tue Jul 2 15:42:13 2013 cynthia 07/01 10 AM 12:00 PM fishione cryotomo prasad 0 Did not recognize the fishione the
second time I tried to put it in.
Tue Jul 2 15:44:51 2013 cynthia 07/01 12:00 PM 4:00 PM tiltrotate cryotomo prasad plastic 1 didn't work because I was havinfg
trouble with my account need
alighment tab and files to load
lost of problems with aperature no
way to load new alignments need and updated account
Tue Jul 2 15:46:25 2013 cynthia 07/02 10:00 AM 3:00 PM tiltrotate rttomo prasad plastic 4 , 2X1 I used Eileens account
Wed Jul 3 17:02:04 2013 eileen 7/3 11:30 AM 5:00 PM tiltrotate rttomo Natalie/Murray 273_2 6
Mon Jul 8 14:03:21 2013 Cindi 07/09 1:30 PM 2:00 PM standard rtimage Brain Sections
Mon Jul 8 16:20:40 2013 morphew 7/8 2:30 PM 4:30 PM tiltrotate rttomo desmin_MTH 1
Tue Jul 9 12:47:30 2013 eileen 7/9 11:30 AM 12:45 PM tiltrotate rtimage yeast examples 0
Tue Jul 9 18:06:49 2013 morphew 7/9 1:00 PM 4:15 PM tiltrotate rttomo desmin MTH 2
Tue Jul 9 18:07:55 2013 eileen 7/9 4:15 PM 6:00 PM tiltrotate rttomo SPC42_MTH_FS_HM20_green 2
Wed Jul 10 18:18:30 2013 Joey 07/10 11:05 AM 6:15 PM tiltrotate rttomo neg stain cyto montages 16
Thu Jul 11 10:06:59 2013 Spilman 7/11 8:15 AM 10:15 PM fishione serviceman test 0 Adjust specimen airlock switches.
Thu Jul 11 18:13:31 2013 Cindi 07/11 2:30 PM 6:15 PM 70 rttomo MTH samples 4
Fri Jul 12 16:45:34 2013 Joey 07/11 9:30 AM 4:45 PM tiltrotate rttomo Neg stained cytos 16
Tue Jul 16 17:34:45 2013 eileen 7/16 8:45 AM 5:30 PM tiltrotate rttomo SPC42_MTH samples 6
Wed Jul 17 16:54:04 2013 eileen 7/17 2:00 PM 5:10 PM 70 rtimage spc42MTH scans 0
Thu Jul 18 09:12:32 2013 Cindi 07/18 8:30 AM 9:15 AM standard rtimage samples for FIB
Thu Jul 18 14:36:18 2013 cynthia 07/19 1:30 PM 2:30 PM tiltrotate rttomo prasad plastic grid broke!
Fri Jul 19 17:47:21 2013 eileen 7/19 8:45 AM 4:00 PM 70 rttomo SPC42-MTH 2 The microscope no longer recognizes
the +/- 70 holder.
Mon Jul 22 13:29:21 2013 cynthia 07/22 10:30 AM 1:30 PM tiltrotate rttomo orasad plastic
Mon Jul 22 17:57:17 2013 eileen 7/22/13 2:30 PM 6:00 PM tiltrotate rttomo spc42_mth 1
Tue Jul 23 11:39:04 2013 Cindi 07/23 9:00 AM 11:40 AM standard rtimage PTA stained samples rebooted computer to boost intensity
Tue Jul 23 17:55:27 2013 Cynthia 07/23 12:30 PM 6:00 PM tiltrotate rttomo prasad plastic 2
Fri Jul 26 10:47:22 2013 Spilman 7/23 8 AM 5:00 PM standard serviceman Test 0 PM due, Made electronic & vaccum checks,
replaced pump oil, water filter, Xray checks,
inspected hoist. re[aced 50 volt supply for HT.
Fri Jul 26 10:48:10 2013 Spilman 7/24 8:00 AM 5:00 PM standard serviceman Test 0 PM due, Made electronic & vaccum checks,
replaced pump oil, water filter, Xray checks,
inspected hoist. re[aced 50 volt supply for HT.
Fri Jul 26 10:48:51 2013 Spilman 7/26 8:00 AM 11:00 AM standard serviceman Test 0 PM due, Made electronic & vaccum checks,
replaced pump oil, water filter, Xray checks,
inspected hoist. re[aced 50 volt supply for HT.
Fri Jul 26 14:16:50 2013 morphew 7/26 11:00 AM 2:30 PM f30cryo cryotomo BPV
Fri Jul 26 16:00:58 2013 Cindi 07/26 2:30 PM 4:00 PM standard serviceman FEG Crash Mary had 2 vacuum crashes. Probably
coincidental. HT died at 268. Ron
coming Monday to look at it again.
Mon Jul 29 17:27:21 2013 Byung-Ho 07 11:30 AM 5:30 PM tiltrotate rttomo C elegans 10
Tue Jul 30 16:44:42 2013 Byung-Ho 07/30 8:30 AM 4:40 PM fishione rttomo Arabidopsis leaf 16
Wed Jul 31 14:25:11 2013 Byung-Ho 07/31 8:40 AM 2:20 PM fishione rttomo Arabidopsis 8
Wed Jul 31 17:09:44 2013 cindi 07/31 2:45 PM 5:15 PM fishione rttomo PTA tests 3
Thu Aug 1 16:21:47 2013 morphew 8/1 10 AM 4:30 PM f20cryo cryotomo bpv_focal pairs
Mon Aug 5 14:45:15 2013 Cindi 08/05 9:00 AM 2:45 PM fishione rttomo FIB samples 5 Fischione rod makes the program ask
you multiple times for what holder
you need.
Tue Aug 6 18:58:37 2013 Cynthia 08/06 10:30 AM 7:00 PM tiltrotate rttomo prasad plastic sucrose 4 duals took a while 20 minutes for vacuum
to go below 20 with t/r, standard
went below right away when I cryo
cycled
Wed Aug 7 17:35:38 2013 Cynthia 08/07 10:15 AM 5:30 PM tiltrotate rttomo prasad sucrose plastic 6
Thu Aug 8 17:30:17 2013 Cynthia 08/08 11:00 AM 5:30 PM tiltrotate rttomo prasad sucrose plastic 5 vacuum went down quickly when I put
in T/R rod
Fri Aug 9 20:35:02 2013 Joey 08/09 10:50 AM 8:30 PM tiltrotate rttomo Whole cytos 32
Mon Aug 12 12:36:49 2013 Cindi 08/12 9:00 AM 12:40 PM tiltrotate rttomo FIB tests 1
Mon Aug 12 18:02:19 2013 eileen and Jayne (Moore lab) 8/12 1:00 PM 6:00 PM tiltrotate rttomo 282 yeast spindles 6
Tue Aug 13 18:41:28 2013 eileen 8/13 9:00 AM 6:40 PM tiltrotate rttomo Candida 102 8
Wed Aug 14 10:38:08 2013 eileen 8/14 8:45 AM 10:30 AM tiltrotate rttomo Candida 102 2
Fri Aug 16 17:04:35 2013 Cindi 08/16 9:00 AM 5:00 PM tiltrotate rttomo FIB Samples 4 noticed vacuum spikes were less and less each
time I inserted rod.
Mon Aug 19 16:55:07 2013 Joey 08/19 10:15 AM 5:00 PM tiltrotate rttomo whole discs 16 Vacuum took a long time to come
down with tilt rotate rod. Removed
rod, added vacuum grease and that
helped a little. Also started
imaging with iptg 1 = 17. Then
seemed to come down after that.
Tue Aug 20 18:13:41 2013 eileen 8/20 8:45 AM 6:10 PM tiltrotate rttomo Desmin-MTH, WTyeast_Au(III)CL,
Candida 102 7
Wed Aug 21 16:24:52 2013 eileen 8/21 8:45 AM 4:30 PM tiltrotate rttomo WT-desmin secondary only, Candida
102 5
Fri Aug 23 16:05:30 2013 Joey 08/22 10:15 AM 4:00 PM tiltrotate rttomo Montage whole discs 16 The last montaged image of the tilt series
contained two images that were completely
black. All other images were fine. I do not this
this was due to a grid bar because they were
right in the center of the montage and the rest
of the other images were fine.
Fri Aug 23 17:00:17 2013 cindi 08/23 9:30 AM 4:30 PM tiltrotate rttomo fib samples 8
Mon Aug 26 17:32:56 2013 eileen 8/26 11 AM 5:30 PM tiltrotate rttomo Moore 282 8
Tue Aug 27 18:20:59 2013 eileen 8/27 9:00 AM 6:20 PM tiltrotate rttomo Moore lab 282 alpha SPC42MTH 5
Fri Aug 30 17:02:55 2013 Joey 08/30 10:00 AM 5:00 PM tiltrotate rtimage checking out grids 0
Wed Sep 4 20:43:24 2013 Joey 09/04 9:30 AM 8:45 PM tiltrotate rttomo montage whole discs 32
Fri Sep 6 15:00:36 2013 morphew 9-6 9 AM 3 PM f20cryo cryotomo bpv 0 3 vac crashes--bailed
Mon Sep 9 16:17:59 2013 morphew 9/9 12:30 PM 4:30 PM f30cryo cryotomo bpv focal pairs 6
Wed Sep 11 16:12:49 2013 Cynthia 09/11 10:00 AM 4:00 PM tiltrotate rtimage dedecker take the cryo box out dingbat :)
Mon Sep 16 15:08:14 2013 morphew 9/16 1 PM 3 PM f30cryo cryotomo bpv 0 vac crash, slow recovery
Fri Sep 20 08:48:48 2013 Ben DE-12 Install 09/17 10:00 AM 7:00 PM standard serviceman try and install DE-12 on scope
Fri Sep 20 08:49:09 2013 Ben DE-12 Install 09/18 10:00 AM 7:00 PM standard serviceman remove DE-12 and put on F20
Fri Sep 20 08:49:59 2013 Ron Spilman 09/18 8:00 AM 5:00 PM standard serviceman Reinstall gatan camera vacuum leak. reinstall gatan
camera. looking better friday
morning
Fri Sep 20 17:17:59 2013 cindi 9/20 8:30 AM 5:15 PM tiltrotate rttomo Selenium bacteria 4
Mon Sep 23 16:50:25 2013 eileen 9/23 9:30 AM 5:00 PM tiltrotate rttomo SPC42_MTH and wt preps 4
Tue Sep 24 14:38:37 2013 Minghua Liu 9/24 10:00 AM 2:30 PM f30cryo cryoimaging Pombe
Wed Sep 25 18:14:04 2013 eileen 9/25 8:30 AM 6:10 PM tiltrotate rttomo Odde Candida 5
Wed Sep 25 19:04:00 2013 Minghua 09/25 6:00 PM 7:00 PM tiltrotate rtimage pombe
Fri Sep 27 15:50:27 2013 eileen 9/27 8:45 AM 3:34 PM tiltrotate rttomo nsp1_MTH 6
Mon Sep 30 18:01:02 2013 Cynthia 09/30 10:30 AM 6:00 PM f20cryo cryotomo desmin wt 7 Nice little F30, Thank you!
Tue Oct 1 17:00:11 2013 eileen 10/1 8:30 AM 5:00 PM tiltrotate rttomo eg5_mts_A3 and wt 6
Wed Oct 2 15:05:59 2013 Cynthia 10/02 9:30 AM 11:30 AM f20cryo cryotomo des mth lousy grids, tooooo thick
Fri Oct 4 13:32:36 2013 eileen 10/4 9:00 AM 1:30 PM tiltrotate rttomo SPC42MTH nano only 2
Fri Oct 4 15:12:23 2013 morphew 10/4 1:30 PM 3 PM f30cryo cryoimaging vitreous sections vac crash
Sat Oct 5 22:10:29 2013 Byungho Kang 10/05 2:00 PM 10:00 PM tiltrotate rttomo C elegans Arabidopsis 20
Sun Oct 6 18:41:00 2013 Byung-Ho Kang 10/06 10:00 AM 6:30 PM tiltrotate rttomo C elegans, Arabidopsis 22 Thanks. F30 worked well.
Tue Oct 8 18:39:54 2013 eileen 10/8 9 AM 6:30 PM tiltrotate rttomo nic96-2mth, wt, Milan_cmk yeast 7
Wed Oct 9 17:38:21 2013 eileen 10/9 8:45 AM 5:45 PM tiltrotate rttomo milan yeast, nic96MTH, chlamy 12
Thu Oct 10 16:38:18 2013 Cindi 10/10 4:15 PM 4:30 PM standard rtimage neg stain MTs
Fri Oct 11 19:02:34 2013 Cynthia 10/11 9:30 AM 7:00 PM tiltrotate rttomo desmin wt and mth tail 9/24 HPF2 7
Mon Oct 14 17:44:54 2013 eileen 10/14 8:45 AM 5:45 PM tiltrotate rttomo Milan yeast and nup1008MTH 6
Mon Oct 14 22:26:45 2013 DNM 10/13 11:00 PM 12:15 AM standard development Testing remote access from 64-bit
version
Tue Oct 15 16:49:25 2013 minghua 10/15 11:00 AM 5:00 PM f30cryo cryoimaging pombe
Wed Oct 16 19:40:55 2013 eileen 10/15 9:00 AM 7:45 PM tiltrotate rttomo nup100 8MTH chlamy 10
Thu Oct 17 12:06:47 2013 DNM 10/18 11:00 AM 12:10 PM standard development testing remote server nd 64-bit
version 1
Thu Oct 17 16:04:17 2013 Cynthia 10/17 1:30 PM 4:00 PM tiltrotate rtimage Prasad Time course t2 screen meter not registering beam
Mon Oct 21 18:54:07 2013 eileen 10/21 8:30 AM 7:00 PM tiltrotate rttomo nup100_8MTH and Milan yeast dmso 10
Tue Oct 22 14:55:08 2013 Cindi 10/22 8:30 AM 2:45 PM tiltrotate rttomo 71+selenium bacteria 8
Tue Oct 22 18:11:38 2013 eileen 10/23 3:00 PM 6:10 PM tiltrotate rttomo wt control nup100 4
Mon Oct 28 16:07:19 2013 Spilman 10/28 9:15 AM 4:30 PM standard serviceman test 0 Communication issue cause by faulty
Right handpanel.
Tue Oct 29 17:54:51 2013 DNM 10/29 11:00 AM 5:00 PM standard serviceman reinstalling Gatan camera and
recovering vacuum
Tue Oct 29 17:55:35 2013 DNM 10/29 5:00 PM 6:00 PM standard development checking if focus change between
GIF and uooer camera changes
intensity
Wed Oct 30 17:46:58 2013 Cindi 10/30 8:30 AM 5:45 PM 70 rttomo spc42mth and se bacteria 4
Thu Oct 31 17:11:42 2013 Cynthia 10/31 12:00 PM 5:00 PM tiltrotate cryotomo prasad 3 cryo box was out. I could not get
comeplete tilt series would stop
after about 112 images at about 48
degrees and claim to be more than
30% out of alignment, yet the
record in 2 of 3 cases was in the
correct area.kept taking pictures
at the same spot doing "reset shift
and realign" and I got poletouch
messages from Microscope Control
program
Fri Nov 1 17:37:39 2013 Cynthia 11/01 10:30 AM 5:30 PM tiltrotate rttomo prasad time course time 2 2x1, 2X2 I could only go -54 to 60 because I
was getting pole touches at tilts
above -54
Mon Nov 4 18:11:17 2013 c page 11/04 3:00 PM 6:00 PM tiltrotate rttomo a3desmin hm20 3 worked great, +_60!
Wed Nov 6 17:25:20 2013 Cynthia 11/06 10:30 AM 5:30 PM tiltrotate rttomo prasad timepoint3 1
Thu Nov 7 17:19:57 2013 Cynthia 11/07 10:15 AM 5:15 PM tiltrotate rttomo prasad timepoint 2 7
Tue Nov 12 17:32:17 2013 eileen 11/12 9 AM 5:30 PM tiltrotate rttomo SPC42MTH post stain tests 3
Wed Nov 13 16:39:10 2013 Cindi 11/13 9:00 AM 4:30 PM tiltrotate rttomo drp1-2xmth-mcherry 5
Thu Nov 14 15:51:53 2013 eileen 11/14 9:00 AM 2:00 PM tiltrotate rttomo DRB1 MTH gold/HM20 3
Fri Nov 15 16:44:17 2013 Cindi 11/15 8:30 AM 9:30 AM standard rtimage cos7
Mon Nov 18 17:43:11 2013 Cynthia 11/18 11:00 AM 5:45 PM tiltrotate rttomo prasad time course2 pt 2 5
Wed Nov 20 18:12:56 2013 eileen 11/20 9 AM 6:10 PM tiltrotate rttomo Yumei/DeCamilli hpf neurons 12
Thu Nov 21 15:35:06 2013 Cynthia 11/21 11:00 AM 3:30 PM tiltrotate rttomo prasad tc2 tp3 5
Fri Nov 22 10:36:07 2013 DNM 11/22 10:00 AM 10:40 AM standard development testing dependence of stage
calibration on rotation angle
Fri Nov 22 17:26:42 2013 eileen 11/21 1:15 PM 5:30 PM tiltrotate rttomo Yumei/DeCamilli Dynamin1KO 4
Mon Nov 25 10:57:50 2013 DNM 11/25 9:55 AM 11:00 AM standard development Testing stage cal with whole image
correlation
Tue Nov 26 15:55:39 2013 Cynthia 11/26 11:00 AM 4:00 PM tiltrotate rttomo prasad tc2,4 4 The beam centering is really off
when you go from low to high mag.
Is this due to the fact that the C2
aperture is off center a bit?
Sun Dec 1 14:43:15 2013 DNM 12/1 8:00 AM 2:45 PM standard development tsting image shift/stage calibration
Mon Dec 2 17:02:41 2013 eileen 12/2 10:30 AM 5:00 PM tiltrotate rttomo Yumei/De Camilli neurons 3
Tue Dec 3 15:41:35 2013 eileen 2/3 9:00 AM 3:40 PM tiltrotate rttomo Yumei/DeCamilli neurons Vogel yeast 5
Wed Dec 4 21:01:57 2013 Johanna 12/4 09:00 AM 9:00 PM f30cryo cryotomo Spermatozoa 11
Thu Dec 5 20:30:47 2013 Johanna 12/5 9:00 AM 8:30 PM f30cryo cryotomo Human spermatozoa 12
Sun Dec 8 20:40:48 2013 Johanna 12/7 11:00 AM 4:00 PM tiltrotate rtimage Human spermatozoa 0
Sun Dec 8 20:43:22 2013 Johanna 12/8 10:30 AM 8:40 PM f30cryo cryotomo Human spermatozoa 9 There was a contamination on the
uppercamera for a while, but
disappeared by it self.
Mon Dec 9 15:51:35 2013 eileen 12/9 8:30 AM 3:50 PM tiltrotate rttomo SPC42-MTH ATM only + imaging 2
Wed Dec 11 18:08:51 2013 eileen 12/11 4:00 PM 6:00 PM tiltrotate rttomo SPC42-MTH ATM acetate 60min 2
Fri Dec 13 15:38:01 2013 Cynthia 12/13/13 11:00 AM 3:30 PM f30cryo cryotomo desmin 0 Grid no good, but scope seems fine
Sat Dec 14 19:31:52 2013 Johanna 14/12 11:00 AM 07:30 PM f30cryo cryotomo Spermatozoa 3
Sun Dec 15 17:29:52 2013 Johanna 15/12 12:00 AM 5:30 PM f30cryo cryotomo Spermatozoa 3
Mon Dec 16 17:44:38 2013 eileen 12/16 10:30 PM 5:40 PM tiltrotate rttomo SPC42_MTH pH expts 5
Tue Dec 17 21:20:53 2013 Johanna 17/12 10:00 AM 09:15 PM f30cryo cryotomo Spermatozoa 5
Wed Dec 18 21:14:56 2013 Johanna 18/12 09:00 AM 09:15 PM f30cryo cryotomo Spermatozoa 10
Fri Dec 20 14:50:42 2013 morphew 12/20 11 AM 3 PM tiltrotate rttomo spc42MTH 0 2 vac crashes
Mon Dec 23 16:39:01 2013 Cindi 12/23 9:00 AM 5:00 PM standard serviceman scope down restart vacuum, no water flow.
Atzlano came and cleaned out lines
with compressed air. Got flow, but
now one of the connectors is
leaking. Scope is down.
Mon Dec 30 11:06:29 2013 Ron Spilman 12/29 8:00 AM 11:00 AM standard serviceman replace water connectors replaced connector, now works
Thu Jan 2 16:47:55 2014 Cindi 01/02 9:00 AM 4:45 PM tiltrotate rttomo bacteria +se 10
Fri Jan 3 17:54:04 2014 eileen 1/3 9:00 AM 5:50 PM tiltrotate rttomo C.elegans anaphase supermontage 3 3x3 montages
Sat Jan 4 15:03:45 2014 eileen 1/4 8:40 AM 3:00 PM tiltrotate rttomo C. elegans anaphase supermontage 3 3x3 montages
Mon Jan 6 17:57:22 2014 eileen 1/6 8:20 AM 5:55 PM tiltrotate rttomo C.elegans anaphase supermontage 4 3x3
Tue Jan 7 18:57:26 2014 eileen 1/7 8:00 AM 7:00 PM tiltrotate rttomo C. elegans anaphase supermontage 6 3x3 montages
Wed Jan 8 15:02:02 2014 Cindi 01/08 8:30 AM 3:00 PM tiltrotate rttomo giardia snmts 6 vacuum at 37, cleaned Oring, OK now
Thu Jan 9 11:16:15 2014 Cindi 01/09 7:15 AM 11:15 PM tiltrotate rttomo giardia snmts 8
Thu Jan 9 16:18:36 2014 Minghua 01/09 11:00 AM 4:30 PM f20cryo cryoimaging Pombe 0 Crashed twice. Used F30 Cryo holder
first and then F20 Cryo holder.
Fri Jan 10 15:24:42 2014 Cindi 01/10 9:15 AM 3:30 PM tiltrotate rttomo giardia snmts 11
Sun Jan 12 16:25:37 2014 eileen 1/12 10:15 AM 4:20 PM tiltrotate rttomo C. elegans anaphase 3 3x3 montages
Mon Jan 13 17:19:15 2014 eileen 1/13 8:45 AM 5:15 PM tiltrotate rttomo C.elegans anaphase supermontage 4 3x3 montages
Mon Jan 20 18:04:51 2014 spilman 1/13 8:00 AM 5:00 PM standard serviceman test 0 Replace objective lens coils &
flush water lines
Mon Jan 20 18:05:04 2014 spilman 1/14 8:00 AM 5:00 PM standard serviceman test 0 Replace objective lens coils &
flush water lines
Mon Jan 20 18:05:15 2014 spilman 1/15 8:00 AM 5:00 PM standard serviceman test 0 Replace objective lens coils &
flush water lines
Mon Jan 20 18:05:26 2014 spilman 1/16 8:00 AM 5:00 PM standard serviceman test 0 Replace objective lens coils &
flush water lines
Mon Jan 20 18:05:39 2014 spilman 1/17 8:00 AM 5:00 PM standard serviceman test 0 Replace objective lens coils &
flush water lines
Mon Jan 20 18:05:54 2014 spilman 1/20 8:00 AM 5:00 PM standard serviceman test 0 Replace objective lens coils &
flush water lines
Wed Jan 22 15:52:39 2014 eileen 1/22 9:00 AM 3:50 PM tiltrotate rttomo Drosophila and yeast 2
Thu Jan 23 16:01:12 2014 MINGHUA 01/23 11:30 AM 4:30 PM f30cryo cryoimaging Pombe crashed when putting the single
tilt rod back in after viewing.
Fri Jan 24 17:36:46 2014 eileen 1/24 8:30 AM 5:30 PM tiltrotate rttomo ellutriated yeast 1 HT and FEG were off when I logged
on. Focus issues.
Tue Jan 28 13:12:46 2014 Cindi 01/27 1:00 PM 2:15 PM standard serviceman alignments
Tue Jan 28 13:33:33 2014 Cindi 01/28 1:00 PM 1:30 PM standard serviceman alignments
Wed Jan 29 10:22:09 2014 Chuck Moe 1/29/14 08:15 AM 10:30 AM standard serviceman alignments EFTEM
Wed Jan 29 13:52:31 2014 Cindi 01/29 1:14 PM 1:45 PM standard serviceman scope down HT is off, but reads 60kV and 50uA
Wed Jan 29 13:52:33 2014 Cindi 01/29 1:14 PM 1:45 PM standard serviceman scope down HT is off, but reads 60kV and 50uA
Thu Jan 30 15:53:32 2014 Spilman 1/30 8:00 AM 4:00 PM standard serviceman none 0 Troubleshoot HT
System down
Tue Feb 4 15:17:51 2014 Spilman 2/3 9:00 AM 3:30 PM standard serviceman Service 0 High dark current when HT Off.
Replace HT feedback board and
aligned scope as needed. tourble
shoot beam instability. Issue went
away and cause not found.
Tue Feb 4 15:18:13 2014 Spilman 2/4 9:00 AM 3:30 PM standard serviceman Service 0 High dark current when HT Off.
Replace HT feedback board and
aligned scope as needed. tourble
shoot beam instability. Issue went
away and cause not found.
Wed Feb 5 17:14:50 2014 eileen 2/5/14 9:00 AM 5:10 PM tiltrotate rttomo Moore 596 yeast 12 worked great!
Fri Feb 7 16:55:20 2014 Cynthia 02/07 11:00 AM 5:00 PM tiltrotate rtimage prasad exp4
Mon Feb 10 17:28:46 2014 Cynthia 02/10 12:00 PM 5:30 PM tiltrotate rttomo prasad exp 4 sample 3 5
Tue Feb 11 18:32:41 2014 eileen 2/11 10:00 AM 6:30 PM tiltrotate rttomo Kristy wt ellute 3
Wed Feb 12 17:02:50 2014 Cynthia 02/12 10:30 AM 5:00 PM tiltrotate rttomo Prasad exp4 sample 6 grid 17c 4
Mon Feb 24 14:10:16 2014 FEI 2/14 8:00 AM 5{00 PM standard serviceman Test 0 Replaced defective lens coil.
reassembled, and aligned.
Mon Feb 24 14:10:32 2014 FEI 2/15 8:00 AM 5{00 PM standard serviceman Test 0 Replaced defective lens coil.
reassembled, and aligned.
Mon Feb 24 14:10:45 2014 FEI 2/16 8:00 AM 5{00 PM standard serviceman Test 0 Replaced defective lens coil.
reassembled, and aligned.
Mon Feb 24 14:10:56 2014 FEI 2/17 8:00 AM 5{00 PM standard serviceman Test 0 Replaced defective lens coil.
reassembled, and aligned.
Mon Feb 24 14:11:15 2014 FEI 2/18 8:00 AM 5{00 PM standard serviceman Test 0 Replaced defective lens coil.
reassembled, and aligned.
Mon Feb 24 14:11:49 2014 FEI 2/21 8:00 AM 5{00 PM standard serviceman Test 0 Replaced defective lens coil.
reassembled, and aligned.
Wed Feb 26 17:43:59 2014 Cynthia 2/25 12:30 PM 6:00 PM tiltrotate rttomo Prasad exp 6 sample 6 grid a2 4
Wed Feb 26 17:45:13 2014 Cynthia 2/26 12:00 PM 5:45 PM tiltrotate rttomo Prasad exp 6 sample 6 grid a2 5
Thu Feb 27 17:27:54 2014 Cynthia 02/27 9:30 AM 5:30 PM tiltrotate rttomo Prasad exp 6 sample 6 grid a4 6 Eucentricity is way off between the
standard and the t/r - don't
remember that being the case. Must
set tilt axis offset by around 6
for t/r rod to be eucentric. Some
issues with auto focus only
measuring a % of actual focus at 60
degrees mag 31k and tilt series
stopped. OK went down in mag to 23K
Fri Feb 28 18:03:59 2014 Cynthia 02/28 11:00 AM 6:15 PM tiltrotate rttomo Prasad exp 6 sample 6 grida3 9
Mon Mar 3 18:00:56 2014 Byungho Kang 03/04 8:20 AM 6:00 PM tiltrotate rttomo Celegans 20 works good none
Tue Mar 4 17:46:41 2014 Byungho Kang 03/05 9:10 AM 5:40 PM tiltrotate rttomo Celegans, Arabidopsis 18 Worked ok other than the vacuum. Vacuum degraded slowly to reach 20
after collecting 2 tilt series.
Wed Mar 5 18:13:16 2014 Byungho Kang 03/05 10:00 AM 06:10 PM tiltrotate rttomo Celegans, Arabidopsis 18 The vacuum degradation problem.
Other than that, it worked ok.
Thu Mar 6 11:08:29 2014 Ron Spilman 03/06 8:45 AM 11:00 AM standard serviceman diagnosing leak in compustage area line needs replacing maybe V8
Thu Mar 6 18:38:46 2014 Cindi, Eva, Alexa 03/06 11:15 AM 6:30 PM tiltrotate rttomo muscle 4
Fri Mar 7 13:20:40 2014 Spilman 3/7 9:00 AM 1:30 PM standard serviceman none 0 Replace & adjust V8 valve, Replace
vacuum line between V8 &V 41
Fri Mar 7 18:00:42 2014 eileen and Ava/Alexa 3/7/14 2 PM 6:00 PM tiltrotate rttomo heart muscle montages 2 1x2
Mon Mar 10 14:52:31 2014 Cindi 03/07 9:00 AM 2:45 PM tiltrotate rttomo neurons 2 sample had to be destained tilt-axis offset high (6 um)
Tue Mar 11 15:45:48 2014 Minghua 03/11 10:00 AM 16:00 PM tiltrotate rttomo Pombe 2
Wed Mar 12 16:54:19 2014 eileen and Eva/Alexa 4/11 8:25 AM 5:00 PM tiltrotate rttomo heart muscle 3 2x2 montages
Fri Mar 14 14:24:37 2014 Cindi,Cynthia,Eva 03/13 9:00 AM 8:45 PM tiltrotate rttomo muscle 10 tilt-axis offset high
Fri Mar 14 18:09:10 2014 eileen 3/14 8:45 AM 6:05 PM tiltrotate rttomo Vogel wt elutriated 11
Tue Mar 18 18:07:30 2014 eileen 3/18 9:00 AM 6:05 PM tiltrotate rttomo wt Jayne/Moore lab and DeCamilli
neurons 3 1x3 montage and 4 regular tilt series
Thu Mar 20 17:32:50 2014 Eileen/Cynthia/Eva 031914 9:00 AM 6:45 PM tiltrotate rttomo Peter Kohl samples 3 1X2 duals, 1 dual
Thu Mar 20 17:34:39 2014 Mary/Cynthia/Eva 03/14 9:45 AM 5:30 PM tiltrotate rttomo Peter Kohl samples cardiomyocytes 3 duals
Fri Mar 21 17:48:13 2014 eileen and Eva 3/21 1:00 PM 5:45 PM tiltrotate rttomo heart muscle Kohl lab 4 1x2 montage
Mon Mar 24 15:35:56 2014 eileen 3/24 8:30 AM 3:30 PM tiltrotate rtimage Yumei/De Camilli neurons 10
Tue Mar 25 15:31:31 2014 eileen 3/25 9:30 AM 3:30 PM tiltrotate rttomo Yumei/De Camilli neurons 8
Wed Mar 26 18:01:24 2014 Mary/Eva/Cynthia 03/26 8:45 AM 6:00 PM tiltrotate rttomo cardiac cells 3, 1X2 duals
Thu Mar 27 18:01:46 2014 Mary/Eva/Cynthia 03/27 9:00 AM 6:00 PM tiltrotate rttomo cardiac cells 2, 1X2 duals 2 duals, 2single
Tue Apr 1 14:15:48 2014 Cindi 04/01 9:00 AM 1:00 PM tiltrotate rttomo neurons 14-day 2 no issues with inserting holder
Wed Apr 2 18:04:46 2014 Mary/Eva/Cynthia 04/02 9:00 AM 6:00 PM tiltrotate rttomo Cardiac cells 4 duals
Wed Apr 2 19:01:06 2014 Minghua 04/02 6:00 PM 7:00 PM tiltrotate rtimage pombe
Thu Apr 3 18:09:23 2014 Cindi/Eva/cynthia 04/03 9:00 AM 6:00 PM tiltrotate rttomo Cardiac Cells 3, 1X3 duals, 1, 1X1 dual
Fri Apr 4 16:14:25 2014 Minghua 04/04 9:50 AM 4:15 PM tiltrotate rttomo Pombe 3
Mon Apr 7 17:14:11 2014 eileen 4/7 11:30 AM 5:10 PM tiltrotate rttomo Jaspersen EM1350 yeast 6
Wed Apr 9 18:07:36 2014 Cindi?Eva/Cynthia 04/09 8:30 AM 6:00 PM tiltrotate rttomo Cardiac Cells 3, duals, 2, 1X2 duals, 1 single axis
Thu Apr 10 18:07:48 2014 Mary/Eva/cynthia 0410 9:00 AM 6:00 PM tiltrotate rttomo Cardiac 4dual, 1, 1X2 dual heard the turbo(?) wind down sound
when I removed the T/R rod
Mon Apr 14 16:30:29 2014 eileen 4/14 8:30 AM 4:30 PM tiltrotate rttomo Jaspersen EM1350 9
Wed Apr 16 17:28:10 2014 eileen 4/16 9:15 AM 5:30 PM tiltrotate rttomo Jaspersen EM1350 10
Thu Apr 17 19:06:07 2014 Matthew West 4/17 9:00 AM 7:00 PM tiltrotate rttomo yeast mutants for Gia Voeltz 12
Fri Apr 18 18:35:16 2014 Cynthia 04/18 10:00 AM 6:30 PM tiltrotate rttomo prasad exp 7 red 3 duals, 4 single axis Eucentricity was way off (LD about
6)with T/R rod as usual of late.
Mon Apr 21 20:46:37 2014 Cynthia 04/21 9:45 AM 8:45 PM tiltrotate rttomo prasad exp 7 blue 6 duals
Thu Apr 24 17:46:38 2014 Cynthia 04/24 10:30 AM 5:30 PM tiltrotate rttomo prasaad exp 7 blue 4 duals 2 singles
Fri Apr 25 13:05:02 2014 morphew 4-25 9 AM 1 PM f20cryo cryoimaging vit sections 0 3 vac crashes
Mon Apr 28 18:08:47 2014 Cynthia 04/30 10:00 AM 6:00 PM tiltrotate rttomo prasad exp 7 blue grid a3 4 duals Yah, no crashes!
Tue Apr 29 12:45:07 2014 Cindi 04/28 8:30 AM 12:45 PM tiltrotate rttomo neurons 4
Wed Apr 30 17:17:47 2014 Cindi 04/29 11:00 AM 5:15 PM tiltrotate rttomo neurons 3
Thu May 1 15:35:12 2014 Cindi 5/1 8:30 AM 5:30 PM tiltrotate rttomo 7 compustage wonky. service coming
next week.
Fri May 2 18:04:21 2014 eileen 5/2 9 AM 6 PM tiltrotate rttomo CDC20 yeast 6 2x2 montages
Wed May 7 13:24:30 2014 Cindi 05/07 8:30 AM 1:30 PM tiltrotate rttomo Bacteria 6
Thu May 8 13:59:58 2014 Cindi 05/08 9:30 AM 2:00 PM tiltrotate rttomo bacteria 5
Fri May 9 16:39:02 2014 eileen 5/9 10:30 AM 4:30 PM tiltrotate rttomo Moore wt anaphase 4
Tue May 13 12:48:20 2014 David 05/12 10:00 AM 11:00 AM standard serviceman fix rotation issue
Tue May 13 12:48:51 2014 Cindi 05/12 11:00 AM 1:00 PM tiltrotate rttomo neurons +10 nm gold 2 david had to fix SerialEM
Tue May 13 17:13:16 2014 eileen 5/13 1 PM 5:10 PM tiltrotate rttomo Moore 596_wt 4
Wed May 14 13:47:25 2014 eileen 5/14 9 AM 1:30 PM tiltrotate rttomo CDC20 6
Thu May 15 15:35:10 2014 morphew 5/15 11 AM 3:30 PM f30cryo cryoimaging vitreous sections U2OS
Fri May 16 22:38:03 2014 Matt West 5/16 9:30 AM 10:30 PM tiltrotate rttomo Yeast mutants 12
Mon May 19 17:10:52 2014 eileen 5/19 8:45 AM 5:05 PM tiltrotate rttomo CDC20 6
Wed May 21 17:22:35 2014 Cynthia 05/21 9:30 AM 5:15 PM tiltrotate rttomo prasad exp7 blue 7 Turbo kicked on each time I removed
the T/R rod. This is unusual. Turbo kicked on each time I removed
the T/R rod. This is unusual.
Thu May 22 15:21:52 2014 morphew 5/22 8:45 AM 3:15 PM tiltrotate cryoimaging pombe
Fri May 23 17:48:05 2014 eileen 5/23 8:45 AM 5:50 PM tiltrotate rttomo CDC20 10
Tue May 27 16:10:26 2014 Cynthia 05/27 10:00 AM 3:30 PM tiltrotate rttomo prasad exp7 blue 7 airlock pumped a second time after
inserting standard rod airlock pumped out a second time
after inserting standard rod at end
of session
Thu May 29 15:38:38 2014 Cynthia 05/29 9:30 AM 3:30 PM tiltrotate rttomo Prasad exp7 blue 9
Fri May 30 17:31:27 2014 Cynthia 05/30 9:30 AM 5:15 PM tiltrotate rttomo prasad blue exp 7 10 putting in standard rod was asked
2X to select rod, and the airlock
was prepumped 2X.
Additionally when I first put in
the T/R rod the system did not
allow me to select a rod, ie it
appeared not to know the rod had
been inserted. I slippped the rod
out slightly and returned it. Then
the rod was detedted.
Mon Jun 9 15:52:33 2014 morphew 6/9 2:30 PM 3:45 PM standard rtimage shuhb boranephosphanate
Tue Jun 10 17:22:41 2014 Cynthia 06/10 11:00 AM 5:15 PM tiltrotate rtimage cytoskeleton Scope worked great! Did have a
blip due to power outage.
Wed Jun 11 17:29:04 2014 Cynthia 06/11 10:00 AM 5:15 PM tiltrotate rttomo Giardia cytoskeletons 1 almost I did Eucentricity with the
standard rod for alignments, then
the lateral displacement was 4.25
when I put in the T/R rod.
Thu Jun 12 17:13:40 2014 Cynthia 06/12 11:00 AM 5:00 PM tiltrotate rttomo Cytoskeletons 1 The F30 was very well behaved
today, I think that is because
Cindor was here!
Wed Jun 18 14:16:24 2014 Cynthia 06/17/14 0:0 AM 0:0 PM standard development NONE please see note On June 11th, the high tension was
off in the morning after an cryo
cycle done the night before
Thu Jun 19 10:59:02 2014 Cynthia 06/19 10:20 AM 10:50 AM standard development trouble shoot I had trouble with 4X4 montage,
looked like every other panel in Y
was out of focus. Was going to do a
1X5 montage to begin to determin
problem, however the beam was,
shaky and pulsing larger then
smaller. During alignments beam was shaking
and pulsing larger, smaller. David
said to call for service.
Tue Jun 24 15:07:04 2014 Chuck Moe 6/23 09:00 AM 5:00 PM standard serviceman service replaced upper obj lens completed upper obj lens
replacement and verified proper
operation
Tue Jun 24 15:08:36 2014 Chuck Moe 6/24 9:00 AM 4:00 PM standard serviceman service conditioned column and HT. Checked
for proper operation after lens
coil replacement conditioned column and HT. Checked
for proper operation after lens
coil replacement
Tue Jun 24 17:28:23 2014 DMN 6/24 1:00 PM 1:01 PM standard development testing log mailing Here is a test message to mary and
eileen
Tue Jun 24 20:11:10 2014 DNM 6/24 1:02 PM 1:03 PM standard development email test 2 Here is a test problem sent to mary,
eileen, and mast
Tue Jun 24 20:13:18 2014 DNM 6/24 1:02 PM 1:03 PM standard development email test 3 Here is a test problem sent to mary,
eileen, and mast@bio3d
Tue Jun 24 20:14:06 2014 DNM 6/24 1:02 PM 1:03 PM standard development email test 4 Here is a test problem sent to
mast@bio3d
Tue Jun 24 20:28:55 2014 DNM 6/24 1:02 PM 1:03 PM standard development email test 5 Here is a test problem sent to mary
and eileen
Tue Jun 24 20:42:01 2014 DNM 6/24 1:05 PM 1:06 PM standard development Test email with subject? Having no subject is a problem too
Thu Jun 26 15:50:10 2014 Chuck 06/26 8:30 AM 4:00 PM tiltrotate rttomo E. coli 2
Mon Jun 30 20:57:37 2014 chuckr 06/30 8:30 AM 9:00 PM tiltrotate rttomo e coli seg mutant minicells 13
Tue Jul 1 20:55:31 2014 chuckr 07/01 10 AM 9 PM tiltrotate rttomo e coli seq mutant minicells 14 tui became non-responsive.
computer reboot required... done by
Chuck of FEI.
Wed Jul 2 07:53:53 2014 Cynthia 06/27 12:00 PM 7:00 PM tiltrotate rttomo Giardia neg stain 1, 4X4
Wed Jul 2 14:18:45 2014 Cynthia 07/02 8:00 AM 2:00 PM tiltrotate rttomo Giardia 1, 4X4
Thu Jul 10 20:33:36 2014 chuckr 07/10 8:00 AM 8:30 PM tiltrotate rttomo e coli seg mutants 10 Good.
Mon Jul 14 13:43:54 2014 David/Eileen 7/10 12 PM 6:00 PM standard serviceman Gif service 0 The GIF was at room temp. Camera
cooling panel in controller burned
out. Switched out the entire camera
controller with spare from Jensen
lab. Cooled to -40
Mon Jul 14 13:46:34 2014 David/Eileen 7/14 9:30 AM 1:30 PM standard serviceman Gif quadrant calibration 0 GIF quadrant correction went well.
New gain reference acquired. Things
seem ok with the GIF
Tue Jul 15 17:45:05 2014 eileen 7.15 3:15 PM 5:40 PM tiltrotate rtimage mapping strategies 0
Wed Jul 16 17:09:38 2014 eileen 7/16 9:00 AM 5:00 PM tiltrotate rtimage GIF tuning, etc. also mapping
strategies 0
Thu Jul 17 14:36:18 2014 morphew 7/17 10 AM 2:30 PM f20cryo cryoimaging synaptophysin Two vac crashes when inserting cryo
rod, one success. Gif needed -10eV
energy shift to align ZLP.
Fri Jul 18 18:30:11 2014 Matthew West 7-18-14 12:00 PM 6:15 PM tiltrotate rttomo Cos mutant tomography 6 The washer for the tilt rotate is
very sticky, so sticky that it bent
and ruined one of my grids just
trying to remove it. Probably just
needs an etoh/acetone
bath...Otherwise the scope ran
flawlessly.
Mon Jul 21 16:50:13 2014 Cynthia 07/21 9:00 AM 4:00 PM tiltrotate rtimage Giardia cyto skel morpholino
Thu Jul 24 08:16:25 2014 chuckr 07/23 8:10 AM 8:30 PM tiltrotate rttomo wt ecoli 10 good!
Thu Jul 24 16:32:20 2014 chuckr 07/24 8:15 AM 4:30 PM tiltrotate rttomo wt e coli 10 great!
Mon Jul 28 16:07:45 2014 eileen 7/26 8:30 AM 4:00 PM tiltrotate rttomo C. elegans supermontage 3 3x3 stage tracking error, but otherwise
fine.
Mon Jul 28 16:09:18 2014 eileen 7/28 8:30 AM 3:00 PM tiltrotate rttomo C. elegans supermontage 2 3x3 stage making clicking sounds.
Autofocus errors, not keeping focus
with montage panels. tilt series
not usable. Service called.
Mon Aug 4 14:36:36 2014 FEI Service 7/29 9:15 AM 5:00 PM standard serviceman checking objective lens 0 objective lens failure. Chuck Moe
replaced upper objective lens and
did alignments 7/29-8/1
Mon Aug 4 14:39:33 2014 eileen 8/4 9:00 AM 12:00 PM standard serviceman Candida 0 Power to objective lens went out
during direct alignments. Called
Chuck/service. Power supply panel
reading 0 volts/0 amps. Chuck
ordered new power supply will
replace tomorrow (8/5).
Mon Aug 25 17:10:40 2014 eileen 8/25 9:00 AM 5:05 PM tiltrotate rttomo Jing pilot tomos 2
Tue Aug 26 15:04:56 2014 Minghua 08/26 1:30 PM 3:00 PM standard rtimage Pombe
Wed Aug 27 17:11:41 2014 eileen 8/27 9:00 AM 5 PM tiltrotate rttomo Wu pombe wt 4 2x1 montage
Fri Aug 29 17:48:13 2014 Matt West 9/29 10:30 AM 5:45 PM tiltrotate rttomo Yeast mutants 8 A combined C2 and beam alignment
problem held me back for an hour,
but thanks to Eileen's help I was
able run my tilt series. Thanks
Eileen for your help!!!
Tue Sep 2 09:27:31 2014 eileen 8/28 10 AM 11 AM standard training Kim Erickson intro 0
Tue Sep 2 17:13:22 2014 eileen and Kim Erikson 9/2 9:00 AM 4:00 PM tiltrotate training Kim's KDE virus-infected cells 2 1x3 montage one panel of montage completely
black two times, then not again.
sample very unstable at high minus
tilt. Needed large drift settling.
Thu Sep 4 15:41:06 2014 eileen and Kim Erikson 9/4 10 AM 3 PM tiltrotate training virus infected cells 2 1x3 montage
Tue Sep 9 17:09:57 2014 kim erickson 9/9 10 AM 4:00 PM tiltrotate rttomo virus infected cells 4 1x3 montages
Wed Sep 10 15:21:55 2014 eileen 9/10 8:45 AM 3:00 PM tiltrotate rttomo Wu pombe mutant 'pilot' 2 1x2 montage
Thu Sep 11 17:45:41 2014 eileen 9/11 9 AM 5:30 PM tiltrotate rttomo Jing antibody treated infected cells 3
Tue Sep 16 14:28:51 2014 Minghua 9/16/2014 11:00 AM 2:30 PM standard rtimage Pombe Crashed once and check the rod's
ring after that. Everything went
fine when putting the rod back in
finally.
Wed Sep 17 15:24:39 2014 morphew 9/18 10 AM 3 PM tiltrotate rttomo ackerson protein crystals 3 one vac crash
Wed Sep 17 17:35:08 2014 Minghua 09/17/2014 4:00 PM 5:40 PM standard rtimage Pombe
Thu Sep 18 17:31:26 2014 eileen 9/18 9 AM 5:30 PM tiltrotate rttomo Candida wt 5
Wed Sep 24 16:23:26 2014 eileen 9/24 9:00 AM 4:15 PM tiltrotate rttomo Candida wt 3
Mon Sep 29 13:13:23 2014 mary/Feldheim 9/29 11 AM 1 PM standard rtimage gold lattice 0
Tue Sep 30 17:03:57 2014 kim erickson 9/30 10 AM 4:30 PM tiltrotate rttomo virus infected cells 4 2x2 montages
Wed Oct 1 16:48:38 2014 kim erickson 10/1 10 AM 4:30 PM tiltrotate rttomo virus infected cells 3 3x3 montages
Thu Oct 2 15:01:03 2014 kim erickson 10/2 10:00 AM 12:15 PM tiltrotate rttomo virus infected cells 1 3x3 montage
Fri Oct 3 15:16:04 2014 eileen 10/3 9:00 AM 3:00 PM tiltrotate serviceman making guest account and testing 1
Tue Oct 7 17:54:29 2014 Eva 10/7 9 AM 5:30 PM tiltrotate rttomo heart muscle 10 single frame, 2 1x2 montage
Mon Oct 13 17:47:36 2014 morphew/eva 10/13 8:30 AM 5:40 PM tiltrotate rttomo muscle 9
Tue Oct 14 17:50:35 2014 eva (kohl) 10/14 8:30 AM 5:45 PM tiltrotate rttomo heart muscle 6 single frame, 4 1x2 montage
Thu Oct 16 17:36:16 2014 eva (kohl) 10/16 8:30 AM 5:30 PM tiltrotate rttomo hear muscle 6 single frame, 2 1x3 montage
Fri Oct 17 16:39:18 2014 eileen 10/17 8:25 AM 4:30 PM tiltrotate rtimage Candida 0
Mon Oct 20 16:34:45 2014 morphew/eva 10/20 8:30 AM 4:30 PM tiltrotate rttomo muscle 6 montages
Wed Oct 22 17:45:19 2014 eva (kohl) 10/22 9 AM 5:30 PM 70 rttomo heart 3,1X3, 1 - 3X3
Wed Oct 22 21:40:40 2014 DNM 10/19 2:00 PM 11:30 PM standard development continuous acquisition
Wed Oct 22 21:41:03 2014 DNM 10/20 4:30 PM 11:30 PM standard development continuous acquisition
Fri Oct 24 18:07:28 2014 eva (kohl) 8/24 8:30 AM 6:06 PM tiltrotate rttomo heart muscle 10
Tue Oct 28 18:04:17 2014 eva (kohl) 10/28 8:30 AM 6 PM tiltrotate rttomo heart muscle 9 (5 single, 2 1x2 montage, 2 3x2 montage The scope was asking for rod
selection again when inserting the
rod. Did not recognize the t/r
holder in the morning, but then no
issues the rest of the day with t/r
or standard rod.
Wed Oct 29 17:24:19 2014 eva (kohl) 10/29 8:30 AM 5:30 PM tiltrotate rttomo Heart muscle All went well with the scope today.
Actual hours of beam time 8
Thu Oct 30 16:35:58 2014 eva 10/30 9 AM 3 PM tiltrotate rttomo heart muscle 5 The grid was not screwed in tightly
in the T/R holder. Grid, hex ring,
washer fell out of holder when
holder was removed. Called service.
Mon Nov 3 17:04:01 2014 eva (kohl) 11/3 9:00 AM 5:00 PM tiltrotate rttomo muscle
Tue Nov 4 17:13:15 2014 eva (kohl) 11/4 8 AM 5:15 PM tiltrotate rttomo heart muscle 6 (including 3x1 adn 2x1 montages)
Wed Nov 5 17:21:09 2014 eva 11/5 9:30 AM 5:00 PM tiltrotate rttomo heart
Thu Nov 6 17:32:31 2014 eileen 11/6 9 AM 5:30 PM tiltrotate rttomo vogel elutriated yeast 10
Mon Nov 10 11:01:02 2014 Chuck Moe 11/10 0830 AM 1100 AM standard serviceman adjustment of obj aperture adjusted obj aperture
Mon Nov 10 17:10:07 2014 eva (Kohl) 11-10 12:00 PM 5:15 PM tiltrotate rttomo muscle
Tue Nov 11 15:53:30 2014 eva (Kohl) 11/11 8:30 AM 3 PM tiltrotate rttomo heart muscle 4 (2x1 and 4x1 montages)
Wed Nov 12 17:26:28 2014 eva/kohl 11/12 9:30 AM 4:30 PM tiltrotate rttomo heart cells 4 duals When I closed Filter control app
got an error message saying start
up Fliter Control before Digital
Micrograph! I did this this morning.
Thu Nov 13 17:15:41 2014 ron 11/13 11 AM 1 PM tiltrotate serviceman problem with objective lens/power
suppy 0 objective lens power off- changed
water filter and scope is back up
Thu Nov 13 17:15:43 2014 ron 11/13 11 AM 1 PM tiltrotate serviceman problem with objective lens/power
suppy 0 objective lens power off- changed
water filter and scope is back up
Thu Nov 13 17:16:47 2014 eva (Kohl) 11/13 1:30 PM 5:30 PM tiltrotate rttomo hear muscle 6 (including montages)
Fri Nov 14 15:36:22 2014 eva (Kohl) 11/14 8:30 AM 3:30 PM tiltrotate rttomo heart muscle 4 (2 1x2 and 2 2x3 montage)
Wed Nov 19 13:14:34 2014 eileen 11/18 8:45 AM 1 PM tiltrotate rttomo Candida- kip1 delete 0 objective lens power went out
again. Service has been called.
Wed Dec 10 16:56:18 2014 eileen 12/10 8:45 AM 5 PM tiltrotate rttomo cooper pombe mutant pink 8
Wed Dec 31 15:49:17 2014 eileen 12/31 8:45 AM 3:50 PM tiltrotate rttomo WT elute Kristy 6 the objective lens chiller went out
and shut off lenses for no apparent
reason. called ron, restarted
chiller and all worked well the
rest of the day.
Tue Jan 6 16:08:40 2015 eileen 1/6 1 PM 3 PM tiltrotate rttomo screening grids for Kamesh's visit 2
Wed Jan 7 17:08:49 2015 eileen/kamesh 1/7 2:00 PM 5:05 PM tiltrotate rttomo rice leaf 2
Thu Jan 8 17:18:43 2015 eileen/kamesh 1/7 8:45 AM 5:00 PM tiltrotate rttomo rice leaf 6
Fri Jan 9 15:58:18 2015 eileen/kamesh 1/9 10 AM 4 PM tiltrotate rttomo rice leaves 2
Tue Jan 13 16:51:38 2015 eileen/cooper 1/13 11:30 AM 4:50 PM tiltrotate rttomo cooper pombe 'yellow' 8
Thu Jan 15 15:11:42 2015 Janet Meehl Fox/Winey 1/15 9:15 AM 2:45 PM tiltrotate rttomo Tetrahymena sfr13Delete basal bodies 6
Fri Jan 16 13:29:58 2015 morphew 1_16 9:40 AM 1:30 PM tiltrotate rttomo Cooper Pombe mut 2
Tue Jan 20 15:19:13 2015 mary(cooper) 1/20 9 AM 3:00 PM tiltrotate rttomo Cooper pombe 4
Wed Jan 21 15:33:46 2015 morphew 1/21 11:00 AM 3:30 PM tiltrotate rttomo cooper wt 2
Fri Jan 23 18:06:17 2015 eileen/cooper 1/23 9:00 AM 6:00 PM tiltrotate rttomo cooper pombe wt 6 2x1 montage
Tue Jan 27 16:01:33 2015 eileen/cooper 1/27 2:00 PM 4:00 PM tiltrotate rttomo cooper pombe 1 1x2 montage
Thu Jan 29 17:28:06 2015 eileen 1/29 9:00 AM 5:30 PM tiltrotate rttomo Cooper wt pombe 8
Fri Jan 30 18:16:17 2015 eileen 1/30 9:00 AM 6:00 PM tiltrotate rttomo Cooper wt pombe and test batch
series 10
Sat Jan 31 14:19:01 2015 DNM 1/31/15 1:30 PM 2:20 PM standard development Fixing diffraction mode setting
through plugin
Fri Feb 13 18:28:32 2015 eileen 2/13 9 AM 6:15 PM tiltrotate rttomo cooper wt 2 vac crash when taking out standard
holder. vac not recover due to
compustage leak. Ron came and
fixed. no other problems
Thu Mar 5 13:51:21 2015 eileen 3/5 10 AM 1 PM tiltrotate rttomo Cooper mutant 6
Mon Mar 9 16:47:47 2015 eva/kohl 3/9/15 8:45 AM 4:45 PM tiltrotate rttomo heart 4(2-1x1, 2 4x1)
Tue Mar 10 18:52:08 2015 eva 3/10 8:45 AM 6:50 PM tiltrotate rttomo heart muscle 1x3 supermontage of 2x3 montages
Wed Mar 11 17:00:02 2015 eva/kohl 3/11 9 AM 4:30 PM tiltrotate rttomo heart muscle 1x2 supermontage of 2x4 montage
Thu Mar 12 17:08:26 2015 eva (Kohl) 3/12 9 AM 4:30 PM tiltrotate rttomo heart muscle 1x2 supermontage of 2x3 montage
Fri Mar 13 16:38:55 2015 eva (Kohl) 3/13 9 AM 3:30 PM tiltrotate rttomo heart muscle 2 2x2 montage
Wed Mar 18 16:41:23 2015 eileen/Cooper 3/18 9 AM 4:30 PM tiltrotate rttomo pombe mutants 'yellow' 8
Thu Mar 19 14:09:22 2015 eileen/cooper 3/19 9:00 AM 1:30 PM tiltrotate rttomo cooper mutant (yellow) 8
Tue Mar 24 16:29:49 2015 eileen/Kohl 3/24 9:00 AM 4:30 PM tiltrotate rttomo higher res cardiac contacts 10
Fri Apr 17 11:51:59 2015 Service (Ron) 4/16 9 AM 5:30 PM standard serviceman service on lens power 0 Power was out to all lenses. Ron
did troubleshooting and found a
loose connection in back panel.
Fri Apr 17 11:52:44 2015 Service (Ron) 4/17 9 AM 11:50 AM standard serviceman service on lens power 0 Power was out to all lenses. Ron
did troubleshooting and found a
loose connection in back panel.
Fri Apr 17 17:33:57 2015 eileen 4/17 1:00 PM 5:30 PM tiltrotate rttomo yeast tomos testing 6
Tue Apr 21 16:41:41 2015 Ron 4/21 9 AM 12:15 PM standard serviceman service 0 Ron reconnected lens power again
Wed Apr 29 14:08:11 2015 DNM 4/29 1:25 PM 2:10 PM standard development test precook montage Installed new SerialEM and scope
plugin
Tue May 5 16:28:15 2015 eileen 5/1/15 9 AM 6 PM tiltrotate rttomo dutcher (ramp down) cnk11-2 9
Tue May 5 16:28:42 2015 eileen 5/5/15 9 AM 4:30 PM tiltrotate rttomo dutcher (ramp down) cnk11-2 6
Fri May 8 16:51:21 2015 eileen/McIntosh 5/8 11 AM 5 PM tiltrotate rttomo mapping PTK2 spindle for
supermontage 1 3x3
Tue May 12 16:49:24 2015 eileen 5/12 10 AM 5 PM tiltrotate rttomo evaluating Laura's COS cells
(Voeltz) 0
Fri May 15 15:20:08 2015 eileen 5/15 10 AM 3:20 PM tiltrotate rttomo PTK2 single axis and GIF testing 1
Thu May 21 15:07:16 2015 kim/eileen 5/21 8:45 AM 3 PM tiltrotate rttomo virus-infected cells 6
Sun May 24 12:11:14 2015 DNM 5/24 9:40 AM 12:15 PM standard development testing pixel size routine and
montage macro 0 scope-signup network wire hooked to
wrong switch
Wed May 27 17:19:21 2015 Kim/eileen 5/27 8:45 AM 5:20 PM tiltrotate rttomo virus-infected cells 6 Training Kim- one hour eot
Thu May 28 15:54:16 2015 kim/eileen 5/28 8:45 AM 3:45 PM tiltrotate rtimage stuff on cell- no tilt series 0
Fri Jun 5 09:51:11 2015 eileen 6/2-6/3 9 AM 5 PM standard serviceman scopes on standby for overnight
electrical work 0
Fri Jun 5 09:51:56 2015 eileen 6/4 9 AM 10 AM standard serviceman scopes on standby briefly- tolin
cancelled 0
Tue Jun 9 17:01:19 2015 Cynthia 06/08 3:00 PM 5:00 PM standard training training myself to use Gif! I was not sucessful tuning the GIF.
Message Found beam on slit but not
on CCD Please check that the TV
retracts and that the shutter works.
I did not log this use into the
equipment log as beam time.
Thu Jun 11 17:40:32 2015 Cynthia 06/11 11:00 AM 5:15 PM tiltrotate rttomo Cos cells Plastic 1 dual only 1.5 hours of beam time
Mon Jun 15 16:33:24 2015 eileen 6/15 11 AM 4:30 PM tiltrotate rtimage mapping PTK2 for supermontage 0
Tue Jun 16 20:52:42 2015 eileen 6/16 9:00 AM 8:50 PM tiltrotate rttomo PTK2 supermontage 6 3x3 montages
Fri Jun 19 17:34:50 2015 Cynthia/Laura 06/15 10:30 AM 5;30 PM tiltrotate rttomo cos cells almost 1 Lots of problems. Insert rod didn't
activate airlock pump, crashed
vacuum. Was a fiber on oring,
removed ok. Image shift(?)issue
above 15K mag Appeared to be image shift movement
that did not correspond to
mouse/stage movement at mags above
15000. Coould not reliably move
stage with mouse. Did tile at 15K,
OK
Mon Jun 22 15:53:55 2015 eileen 6/22 11:00 AM 3:50 PM standard serviceman redid image shift calibration and
redid DM gain reference 0 There was a bad image shift
calibration in the short term file.
redid this from scratch.
Tue Jun 23 17:24:45 2015 eileen 6/23 12:00 PM 5:30 PM tiltrotate rttomo dutcher DB947 6
Wed Jun 24 18:39:41 2015 Cynthia/Laura 06/24 9:30 AM 6:30 PM tiltrotate rttomo cos cells 3.5 duals Everything went fine!
Tue Jun 30 17:16:46 2015 morphew 6/30 2:30 PM 5:15 PM tiltrotate rttomo Ptk 2
Wed Jul 1 13:09:43 2015 morphew 7/1 10:30 AM 12:15 PM tiltrotate rtimage ptk
Wed Jul 1 18:05:44 2015 eileen 7/1 3 PM 6 PM tiltrotate rttomo dutcher DB20 9
Thu Jul 2 15:47:04 2015 eileen 7/2 10 AM 3:00 PM tiltrotate rttomo dutcher DB20 9
Wed Jul 8 12:30:21 2015 Chuck Moe 7/8 0830 AM 1230 PM f30cryo serviceman stage lockup recovery Removed holder in safe state. reset
EPD sensors on stage for proper id
of holders. Tested cryo holder at
60 degrees for issues. none noted.
Thu Jul 9 12:06:34 2015 eileen 7/7 10 AM 2 PM tiltrotate rttomo dutcher DB19 7 Holder jammed at high tilt
Thu Jul 9 12:07:07 2015 eileen 7/8 3 AM 5 PM tiltrotate rttomo dutcher DB19 3
Thu Jul 9 12:07:33 2015 eileen 7/8 3 AM 5 PM tiltrotate rttomo dutcher DB19 3
Fri Jul 10 15:33:36 2015 kim 7/9 10 AM 4 PM tiltrotate rttomo virus 4
Fri Jul 10 15:34:01 2015 kim 7/10 10 AM 4 PM tiltrotate rttomo virus 6
Mon Jul 13 09:15:11 2015 Chuck Moe 7/13 0730 AM 0930 AM standard serviceman service on P15 gauge Replaced P15 gauge and restarted
vacuum system
Tue Jul 14 16:08:43 2015 morphew 7/14 2:30 PM 4:15 PM tiltrotate rttomo ptk fix/freeze 2
Wed Jul 15 16:48:17 2015 Cynthia/Laura 0715 10:00 AM 4:30 PM tiltrotate rttomo cos cells transfected 1
Fri Jul 17 15:01:44 2015 morphew 7/17 2:30 PM 3 PM standard rtimage quick check
Mon Jul 20 16:30:47 2015 Cynthia 0720 12:00 PM 3:30 PM tiltrotate rtimage cos cells disc 3
Fri Jul 24 13:03:52 2015 morphew 7/24 11 AM 1 PM tiltrotate rttomo ptk 1
Tue Jul 28 15:28:25 2015 morphew 7/29 1 PM 3:15 PM tiltrotate rttomo mcintosh 2
Thu Jul 30 10:43:36 2015 eileen 7/30 10 AM 11 AM tiltrotate rttomo checking settings and images
(service/maintenance) 1
Thu Jul 30 15:53:51 2015 janet 7/29 12 PM 4 PM tiltrotate rtimage mth spc29 0
Thu Jul 30 15:54:31 2015 eileen 7/30 10 AM 3 PM tiltrotate rttomo ptk2 chem/hpf 3x3 montage 1
Fri Jul 31 17:24:10 2015 DNM 07/31 2:30 PM 5:30 PM standard development realign to item bugs 0
Sat Aug 1 13:25:54 2015 DNM 8/1 9:00 AM 1:30 PM standard development realign to item
Tue Aug 11 16:28:29 2015 eileen 8/11 3 PM 4:30 PM tiltrotate rtimage setting up PTK2 0
Wed Aug 12 20:53:15 2015 eileen 8/12 8:45 AM 9 PM tiltrotate rttomo PTK2 supermontage sec2 McIntosh 2x3 super of 3x3 montages
Fri Aug 14 14:46:22 2015 eileen 8/14 9 AM 2:30 PM tiltrotate rttomo organoids 5
Mon Aug 24 16:18:05 2015 morphew 8/23 2:30 PM 4 PM tiltrotate rttomo PTK DeLuca 1
Tue Sep 1 10:30:43 2015 DNM 9/1 10:30 AM 10:31 AM standard development test log
Tue Sep 1 10:39:03 2015 DNM 9/1 10:30 AM 10:31 AM standard development test log
Wed Sep 2 11:57:30 2015 eileen/McIntosh 9/2 9:15 AM 11:45 AM tiltrotate rttomo Deluca/McIntosh wt kinetochores 4
Thu Sep 3 13:55:03 2015 eileen/McIntosh 9/3 9:00 AM 1:30 PM tiltrotate rttomo McIntosh/Deluca 9A3 7
Tue Sep 15 16:58:45 2015 eileen 9/15 2 PM 5:00 PM tiltrotate rttomo Deluca/McIntosh 1.5 hours 2 CCD camera had warmed. Let cool for
several hours before starting
session.
Wed Sep 16 16:58:05 2015 eileen 9/16 1:30 PM 5:00 PM tiltrotate rttomo mcintosh/deluca 9A1 and wt1 6
Tue Sep 22 16:20:16 2015 eileen 9/22 11:30 AM 3:00 PM tiltrotate rttomo Eric Cole WT TET 4 3x1 2 single
Wed Sep 23 12:29:01 2015 eileen 9/23 10:30 AM 12 PM tiltrotate rttomo Eric Cole WT Tet 2 1x2 montage
Tue Sep 29 17:23:36 2015 eileen 9/29 4:00 PM 4:45 PM tiltrotate rttomo Shanshan block12 2 vac took a while to come down
Wed Sep 30 14:48:38 2015 eileen 9/30 12:45 PM 2:30 PM tiltrotate rttomo Shanshan block12 cell1 3
Fri Oct 2 17:16:43 2015 DNM 10/1 12:01 PM 3:30 PM standard development Defocus from FFT
Fri Oct 2 17:17:04 2015 DNM 10/2 12:01 PM 4:00 PM standard development Defocus from FFT
Wed Oct 7 16:38:52 2015 eileen 10/7 2:00 PM 4:30 PM tiltrotate rttomo Shanshan block11 cell1 5
Fri Oct 9 16:15:44 2015 eileen 10/9 12:30 PM 3:30 PM tiltrotate rttomo Cole Tet 45min and autoacquire
testing 3
Sun Oct 11 12:15:37 2015 eileen 10/11 9:30 AM 12:00 PM tiltrotate rttomo Eric Cole class demo 1 1x2 montage
Wed Oct 21 16:11:16 2015 eileen 10/21 10:30 AM 3:00 PM tiltrotate rttomo Shanshan B1_1 adn B_2 plus auto
testing 6
Fri Oct 23 14:07:09 2015 eileen 10/23 11 AM 1:30 PM tiltrotate rttomo Shanshan B3_1 and automation testing 4
Fri Oct 30 15:35:11 2015 Chuck Moe 10/30 07 AM 16:00 PM standard serviceman PM PM PM
Mon Nov 9 14:26:41 2015 eileen 11/9 12:00 PM 2:15 PM tiltrotate rttomo Shanshan B3 cell1 grid I3 4
Mon Nov 9 14:28:24 2015 Chuck (service) 11/5 7 AM 2:30 PM standard serviceman replaced relay on objective lens
power supply 0
Mon Nov 9 14:30:08 2015 Chuck (service) 11/9 8 AM 11:00 AM standard serviceman reset network connector on computer
and replaced air lines from
compressor 0
Tue Nov 10 17:19:37 2015 eileen 11/10 8:30 AM 4:30 PM tiltrotate rttomo PTK2 supermontage section3 2x2 superof 3x3 montage
Wed Nov 11 17:05:34 2015 eileen 11/11 8:45 AM 4:30 PM tiltrotate rttomo PTK2 supermontage section4 2x2 super of 3x3 montages
Thu Nov 12 15:25:14 2015 eileen 11/12 9:00 AM 12:30 PM tiltrotate rttomo PTK2 montage reshoot 2 3x3 montage
Wed Nov 18 16:47:23 2015 DNM 11/17 11:30 AM 4:50 PM standard development high defocus stage calibrations
Mon Nov 23 06:35:19 2015 DNM 11/22 10 AM 8 PM standard development Focus-dependent mag testing Stage positioning seems to have 1-2
micron errors even with backlash
correction
Thu Dec 3 14:50:36 2015 eileen 12/3 2 PM 2:30 PM standard rtimage Tour with Linda Shoemaker Regent 0
Mon Dec 7 16:54:02 2015 Cynthia/Laura 12/07 10:30 AM 4:45 PM tiltrotate rttomo cos cells scram, drp1 kd 3 duals beam/crud, loaded new alignments
(5/11/15)and was OK! 5 hours of
actual scope time.
Tue Dec 8 18:58:01 2015 Cynthia/Laura 12/08 12:30 PM 6:30 PM tiltrotate rttomo cos cells exp 8 6 duals w9orked great!
Thu Jan 28 11:35:14 2016 Chuck Moe 1/28 07:30 AM 11:30 AM standard serviceman FEG replacement replaced FEG and saved new
alignments no issues
Tue Mar 1 16:59:02 2016 eileen 3/1 11:00 AM 5:00 PM tiltrotate rttomo plunge pombe 7 2x1 montage
Wed Mar 2 13:56:57 2016 eileen 3/2 9:30 AM 2:00 PM tiltrotate rttomo plunge pombe 6 (4 single frame, 2 1x2 montage)
Mon Mar 7 16:42:34 2016 eileen 3/7 10:30 AM 4:30 PM tiltrotate rttomo plunge pombe 5 2x1 montage
Tue Mar 8 17:20:20 2016 eileen 3/8 9:30 AM 5:15 PM tiltrotate rttomo pombe plunge 6
Wed Mar 9 11:15:28 2016 eileen 3/9 9:00 AM 11:00 AM tiltrotate rttomo plunge pombe 2 1x2 montage
Thu Mar 10 17:25:32 2016 DNM 3/10 11:30 AM 5:30 PM standard development testing for low mag montage crash 0
Tue May 24 16:04:51 2016 Eileen (Natalie) 5/24 11:30 AM 3:45 PM tiltrotate rttomo maize meoitic spindle 3 2x2 montage
Thu May 26 15:50:27 2016 DNM 5/26 3:00 PM 5:00 PM standard development looking at diffraction and lateral
offset 0 Tilt axis offset is not ~4.5
microns and should be fixed
Thu May 26 15:51:23 2016 DNM 5/26 1:00 PM 4:00 PM standard development looking at astigmatism with Garry
offset 0
Tue Dec 13 09:18:11 2016 DNM 12/12 9:30 PM 9:20 AM standard development Testing focus limiting in tilt
series
Wed Jan 11 17:38:07 2017 Matthew West 1/11/17 12:45 PM 5:45 PM fishione rttomo mutant yeast 6 Crashed the f-20 column, but was
able to work from the f-30. A few
Serial EM glitches, but everything
corrected itself if given a moment.