matchvol(1) General Commands Manual matchvol(1) NAME matchvol - transform a volume to match another with a general linear transformation SYNOPSIS matchvol [options input_file output_file DESCRIPTION Matchvol will transform a volume using a general linear transformation. Its main use is to transform one tomogram from a two-axis tilt series so that it matches the other tomogram. To do so, it can combine an initial alignment transformation and any number of successive refining transformations. The program uses the same algorithm as Rotatevol for rotating large volumes. The format of the 3D transform when stored in a file is to consist of 3 lines: a11 a12 a13 dx a21 a22 a23 dy a31 a32 a33 dz which specify a transformation for getting from a location in the input volume to a location in the output volume: xo = a11 * xi + a12 * yi + a13 * zi + dx yo = a21 * xi + a22 * yi + a23 * zi + dy zo = a31 * xi + a32 * yi + a33 * zi + dz where (xo, yo, zo) are coordinates relative to the center of the output volume, and (xi, yi, xi) are coordinates relative to the center of the input volume. OPTIONS Matchvol uses the PIP package for input (see the manual page for pip) and can take input interactively for options that existed when it was converted, to maintain compatibility with old command files. The following options can be specified either as command line arguments (with the -) or one per line in a command file or parameter file (with- out the -). Options can be abbreviated to unique letters; the cur- rently valid abbreviations for short names are shown in parentheses. -input (-inp) OR -InputFile File name Input image file to transform -output (-ou) OR -OutputFile File name Output file for transformed volume -inverse (-inv) OR -InverseFile File name Output file in which to write the inverse of the combined trans- formation. This output is optional. -tempdir (-t) OR -TemporaryDirectory Text string Directory to use for temporary files. The default is that the temporary files will be placed in the current directory. -size (-s) OR -OutputSizeXYZ Three integers X, Y, Z dimensions of the output file (Default is size of input file) -center (-ce) OR -CenterXYZ Three floats X, Y, Z index coordinates of the center of the region to trans- form (Default is center of input file). Coordinates are num- bered from zero. -xffile (-x) OR -TransformFile File name Name of file with 3D transform to apply. Multiple transforms are applied in the order that they are entered, and before any 3DTransform entries. (Successive entries accumulate) -3dxform (-3) OR -3DTransform Multiple floats A 3D transform to apply, consisting of 12 values on one line (a11, a12, a13, dx, a21, a22, a23, dy, a31, a32, a33, dz). Mul- tiple transforms are applied in the order that they are entered, and after any TransformFile entries. (Successive entries accu- mulate) -order (-or) OR -InterpolationOrder Integer Order of interpolation to use. Currently only quadratic (2) and linear (1) interpolation are available; the default is quadrat- ic. -chunk (-ch) OR -ChunkSizesForHDF Three integers Size of chunks in X, Y, and Z for output to an HDF file orga- nized in chunks. Storing data this way will prevent the need to output cubes of transformed data to scratch files and read them back in to make the final output, when the volume is too large to fit in the allowed memory. Enter 0,0,0 for the default size, which is for chunks the same size as the cubes that would ordi- narily be written to scratch files, and for no chunks at all if data are being handled in one cube per layer. If a specific size is entered for one or more axes, then the program will first determine the division into cubes, and then make the chunk size be the minimum of the cube size and the specified value for each axis. When using chunks, the memory is limited to 15000 megabytes even if a larger value is entered. -memory (-m) OR -MemoryLimit Integer Amount of memory to allocate for the major arrays needed by the program, in megabytes. This always includes memory for input images and may include memory for a stack of output slices, depending on the orientation of the output. The default is the minimum of system memory minus 1 GB, 60% of system memory, and 12 GB for system memory up to 30 GB, and 40% of system memory above 30 GB, but at least 768 MB. -verbose (-v) OR -VerboseOutput Integer 1 for diagnostic output -param (-p) OR -ParameterFile Parameter file Read parameter entries as keyword-value pairs from a parameter file. -help (-h) OR -usage Print help output -StandardInput Read parameter entries from standard input. INTERACTIVE INPUTS If the program is started with no command line arguments, it reverts to interactive input with the following entries: Name of the input file to be transformed Name of the output file for the transformed volume Path name of directory (for example, /usr/tmp) where temporary files can be placed, or Return to have files placed in the current directory X, Y, and Z dimensions of the output file, or / to accept the default values, which are NZ, NY, and NX of the input volume (a 90-degree rotation about the Y axis) Number of successive transformations to combine and apply For each transformation, either enter the name of a file with the transformation, or enter a Return, then enter the 12-element transformation directly. Transform files need not all occur before direct entries. Name of a file in which to place the inverse of the combined transformation, or Return for no such output HISTORY Written by David Mastronarde, 1995 Converted to PIP/autodoc 10/10/03 Converted to Fortran 95, parallelized with OpenMP, 6/14/09 BUGS Email bug reports to mast at colorado dot edu. IMOD 5.2.0 matchvol(1)