imodfillin(1) General Commands Manual imodfillin(1) NAME imodfillin - fill in missing contours in IMOD model or remove inside contours SYNOPSIS imodfillin [options] input_model output_model DESCRIPTION Imodfillin will fill in contours in an IMOD model on sections that were skipped during modeling, or remove contours that are inside others (filling in the inside in another sense). For the filling operation, it interpolates a contour between existing ones using information in the object mesh created by Imodmesh or 3dmod. Each interpolated contour will fall on the surface connecting existing contours. It is thus necessary to get objects meshed before running imodfillin. Since you need to have a mesh between contours on non-adjacent sections, you should use one or more meshing options to connect across gaps in the data. If sections have either a complete set of contours or no con- tours at all, then the -s (Skip) option will suffice. If the pattern is less rigid than this, use the -P (Passes) option in addition (or exclusively), where the number of passes that you specify with this option equals the maximum number of sections of separation between con- tours that you wish to connect. Do not use any Imodmesh option that would set the z increment to greater than 1 (i.e., -l, -i, or -z (Z inc in 3dmod), for then Imodfillin will probably create contours on sec- tions that already have them. Objects containing meshes from an isosurface and no contours will be analyzed differently to extract contour trajectories from lines in the mesh that lie exactly on Z planes. It does not interpolate between these planes, so if the isosurface is made with binning, the contours will not be on every Z plane. Parts of the surface that are cut off by the bounding box will result in open contours. Some of these may have gaps in them, but closed contours should generally be complete. The resulting contours are unlikely to mesh properly except for compact, well-separated surfaces. The default point reduction with a tolerance of 0.25 pixel may be too high for isosurfaces around small structures. By default, the program will attempt to fill in all closed-contour objects that have meshes, but will skip open-contour objects. Use the -o option to specify a subset of objects to be done, or to force it to do particular open-contour objects. The created contours will inherit any surface or time information con- tained in the meshes. If your original contours have surface numbers, be sure to mesh with the -S option to preserve this surface information in the meshes, so that the interpolated contours will have the same surface numbers as the original ones. The removal of inside contours is activated by the -I option, which specifies whether to remove just contours at even levels (i.e., inside- facing ones) or all interior contours. In this case, the other options have no effect, except -o to specify a list of objects and -z to restrict the range of Z values over which the removal happens. OPTIONS -e Place new contours in existing objects. Use this option if you do not plan to check and modify the interpolated contours. -n Place new contours in new objects; this is the default, so that contours can be checked conveniently. One new object will be created for each object that is being filled in. These contours may be moved into the proper object either one by one or all at once via the Edit-Contour-Move dialog and M hotkey in 3dmod. -o list Do operations only on objects in the list of numbers. The list can consist of comma-separated ranges (e.g., 1,7-11,13-17,19). Without this option, all closed contour objects with meshes are filled in. -i value Set the Z increment for filling in contours. This option has two effects: the program will fill in only gaps that are wider than the given value, and it will insert new contours at this given Z increment instead of on every section. -R value Resolution or tolerance for point reduction in the generated contours. Points are eliminated if they are within this dis- tance of the lines connecting the remaining points, just as with the -R option in Imodmesh. Values in the range 0.25 to 0.5 are often useful; the default is 0.25. -I value Enter 2 to remove all contours contained inside other contours, or 1 to remove contours that are immediately inside an outward- facing one, i.e, ones that constitute the inside surface of a donut-shaped object. -z start,end Range of Z values (numbered from 0) within which to remove con- tours when the -I option is entered. FILES If the model file already exists, it becomes a backup file with ~ added to its name. AUTHOR David Mastronarde SEE ALSO 3dmod, Imodmesh BUGS Email bug reports to mast at colorado dot edu. IMOD 5.2.0 imodfillin(1)