Boulder Laboratory for 3-Dimensional Electron Microscopy of Cells ASSEMBLEVOL(1) ASSEMBLEVOL(1) NAME assemblevol - assemble an image file from an array of subvolumes SYNOPSIS assemblevol DESCRIPTION Assemblevol will assemble a single MRC file from separate files that form an array of subvolumes in X, Y, and Z. In effect, it can take montaged images and compose them into single images, just as Reducemont can, but its advantage is that it can take the images from multiple files. Its primary use is for reassembling a tomogram after it has been chopped into pieces, using the coordinates output by Tomopieces. A second use to concatenate reprojections computed in parallel by Tilt. Inputs to the program: Name of output file for the assembled volume Number of input files in the X, Y and Z dimensions For each position in X, enter a line with the starting and ending X index coordinates (numbered from 0) to be extracted from the files at that position. These coordinates should be chosen to eliminate any overlap between the files at successive positions. For example, if there are two files in X, each 1000 pixels wide, and they overlap by 20 pixels, you would enter 0,989 and 10,999. If there is no overlap between any of the files in X, you can enter 0,0 for each position, but you can not enter 0,0 for some positions and actual coordinates for others. Similarly, for each position in Y, enter a line with starting and ending Y index coordinates to be extracted from files at that position. Again, if there is no overlap between any of the files in Y, you can enter 0,0 for each position. For each position in Z, enter a line with starting and ending Z index coordinates to be extracted from files at that position. If there is no overlap between any of the files in Y, you can enter 0,0 for each position. The input file names, one per line, in order by increasing X, then increasing Y, then increasing Z. For example, if there are files only in X and Z, enter all of the files in the first row in Z in order by increasing X, then the files in the second row, etc. HISTORY Written by David Mastronarde 3/1/01