Boulder Laboratory for 3-Dimensional Electron Microscopy of Cells
EXTRACTPIECES(1) EXTRACTPIECES(1)
NAME
extractpieces - Extract piece coordinates from header of image file
SYNOPSIS
extractpieces input_image_file output_piece_list_file
DESCRIPTION
EXTRACTPIECES will extract piece coordinates from the header of
an image file, if they are present, and produce a file with those
coordinates (a piece list file). In addition, it can extract coordinates
from an image metadata file produced by SerialEM. The metadata file
must have the same number of sections as the Z size of the image file.
Extractpieces uses the PIP package for input (see the manual page for
pip). Thus, the following options can be specified either as command line
arguments (with the -) or one per line in a command file (without the -):
-input OR -InputFile File name
Image file with header information to extract. If this option is not
entered, the first non-option argument will be taken as the input file.
-output OR -OutputFile File name
Output file for header values. If this option is not entered, the second
non-option argument will be taken as the output file.
-mdoc OR -MdocMetadataFile
Obtain values from a metadata file named with the name of the input file
plus '.mdoc', instead of from the extended header of the image file. A
file with this name is generated by SerialEM when an option is selected to
save extra information in a metadata file, or when the coordinates are too
large to fit in the extended header.
-other OR -OtherMetadataFile File name
Obtain values from the metadata file with the given name, instead of from
the extended header of the image file. With this option, no input image
file needs to be supplied; but in this case you must specify the output
file with "-output" instead of as a non-option argument.
-help OR -usage
Print help output
-StandardInput
Read parameter entries from standard input.
If the program is started with no command line arguments, it reverts to
interactive input with the following entries:
Image file with piece list information in header
Output file for piece coordinates
HISTORY
Written by David Mastronarde, October 1999