excludeviews(1) General Commands Manual excludeviews(1) NAME excludeviews - Reversibly remove views from a tilt series stack SYNOPSIS excludeviews options stack_name DESCRIPTION Excludeviews removes images from a stack, saves them in another stack, and replaces the original stack with one without those images. This operation is most useful for excluding views from a tilt series. It works with montaged or single-frame images. Information about the operation is kept in a file with extension ".info"; this allows the operation to be run in reverse to restore the original image file. Because of this reversibility, it is safe to remove the original files with the -delete option. The program can be run repeatedly (up to 100 times), and each succes- sive exclusion is similarly reversible. This capability is achieved by including an iteration number in the names of the ancillary files pro- duced on each run. The program searches for the info file with the highest number and applies the next highest number in another exclu- sion, or restores the exclusion on that iteration. When running a new exclusion, the list of views entered are numbered by their positions in the current stack, not the original one. Ancillary data are always preserved in both directions of operation. Specifically, if there is a ".rawtlt" file with tilt angles or a ".mdoc" file with metadata, it will be split in two or recombined appropriately. If the stack is identified as a montage by internal data or by giving the -montage option, a ".pl" file with piece coordi- nates will also be split or recombined. Metadata will also preserved appropriately when the input and/or output files are HDF files; specif- ically, metadata in an HDF file will be placed into an .mdoc file when the output is MRC type, metadata in a .mdoc file associated with an MRC file will be stored as attributes when the output is an HDF file; and attributes will be transferred between HDF files with appropriate modi- fications. This transfer is accomplished with the -mdoc option to New- stack(1) or Edmont. OPTIONS Excludeviews uses the PIP package for input (see the manual page for pip). Options can be specified either as command line arguments (with the -) or one per line in a command file (without the -). Options can be abbreviated to unique letters; the currently valid abbreviations for short names are shown in parentheses. -stack (-s) OR -StackName File name Name of the image stack to operate on, from which a rootname and extension can be derived. However, if restoring with the -orig option, this should be the rootname and extension without the "_orig". This is a required entry; if it is not entered with this option is not entered, the first non-option argument will be taken as the image stack name. -views (-v) OR -ViewsToExclude List of integer ranges List of views to exclude, numbered from 1. This entry is required unless -restore is entered. When restoring a stack, it is not necessary to enter the view list, and it is better not to, if an info file is present from when the views were first excluded. However, if there is no info file, a view list is needed; this would be the original list of views given for the exclusion. -montage (-m) OR -MontagedImages The image stack consists of montaged images. If this option is not entered, the program will detect whether a file is a montage automatically, if the piece coordinates are contained in the header of an MRC file, the attributes of an HDF file, or an associated ".mdoc" file (one whose name consists of the stack name plus ".mdoc"). Thus, this entry is not needed unless the piece coordinates are contained only in a piece list file. It is also not needed when restoring a stack if there is an info file present. -delete (-d) OR -DeleteOldFiles Delete the redundant image and associated files after an opera- tion is successfully completed instead of retaining them. Specifically, after excluding views, the original image stack and any associated files that are replaced are renamed with "_allviews#" by default (where "#" is the iteration number), and will be deleted with this option. After restoring excluded views, the existing files are renamed with "_keptviews#" by default; whereas with this option, both these files and the ones for excluded views named with "_cutviews#" will be deleted. -restore (-r) OR -RestoreFullStack Combine retained views in the current stack file with excluded views in the latest "_cutviews#" file to restore the full stack present before the last iteration. If an info file is present, the -views and -montage options should not be entered. If -views is entered, the info file is ignored and -montage will be needed if piece coordinates are only in a piece list file. If multiple iterations of exclusion have been run, the program needs to be run with this option that number of times to get back to a stack with all of the original views. -orig (-o) OR -OriginalStack Restore excluded views into the original stack before X-ray removal. By default, the current file entered with -stack will be combined with the excluded views; with this option, the orig- inal file "rootname_orig.ext" will be combined with the excluded views and the combined file will be given that name. -PID Print process ID -help (-h) OR -usage Print help output -StandardInput Read parameter entries from standard input FILES Three different strings are inserted before the extension to identify files on a particular run, where "#" is the iteration nunber: "_cutviews#" for views removed from a stack; "_keptviews#" for the retained views, "_allviews#" for a file with all the views. The info file on iteration # is named "rootname_cutviews#.info". After an oper- ation is reversed, the info file is renamed to "root- name_cutviews#_old.info". AUTHOR David Mastronarde SEE ALSO newstack, edmont BUGS Email bug reports to mast at colorado dot edu. IMOD 4.11.0 excludeviews(1)