colornewst(1) General Commands Manual colornewst(1) NAME colornewst - Run Newstack command on a color file SYNOPSIS colornewst [options] input_file output_file DESCRIPTION Colornewst is a Python script to run Newstack operations on a color file by splitting the file into components with "clip splitrgb", run- ning Newstack, and recombining the components with "clip joinrgb". Most options to Newstack can be included, but not -fileinlist, -fileoutlist, -split, -replace, -numout, -skip, -blank, -onexform, -exclude, -input, or -output. There must be only one input file and one output file, specified as the last two arguments on the command line. If placing options in a command file or script, always use at least three letters for the Newstack options. The input file can be any type readable by IMOD programs generally, namely MRC, TIFF, HDF, or JPEG. The output file will be an MRC file, unless the environment variable IMOD_OUTPUT_FORMAT is set to TIFF, HDF, or JPEG. The latter will work only for single-image byte or RGB files, not stacks or other data modes; in the latter cases the output will be MRC despite the variable setting. See the imodenv man page for other environment variables to control compression type and quality when getting TIFF or JPEG files in this way. If the input file is a TIFF file, you can use the -cntiff option to tell the program to con- vert the input file to an MRC file with Tif2mrc using no options, and convert the processed MRC file to TIFF with Mrc2tif using the -s option. If any more complicated conversions are desired, run the con- versions separately with the desired options. If the input file is gray-scale instead of color, Newstack will be run directly on it with the given options. Colornewst itself takes the following options, which cannot be abbrevi- ated. These options can be intermixed with the Newstack options. Two leading dashes are accepted, just as for the Newstack options. -cntiff Convert the input file from TIFF to MRC with Tif2mrc, process it, and convert the processed file to TIFF. By default, TIFF files will be read by Newstack and an MRC file will be pro- duced. -cntempdir dir Path to directory to use for temporary files, namely the red, green, and blue components produced by "clip splitrgb" and files for each component processed by Newstack. The default is the standard temporary directory for IMOD commands. -cnmaxtemp # Maximum amount of space to use in the temporary directory, in megabytes (floating point values allowed). The default is 1024. When processing the whole file at once would require more than this amount of space, the program will process the file in chunks. -cnverbose Pass on the printed output from running Newstack and the TIFF conversion programs, if any. The default is for this output to be swallowed. FILES Temporary files are named starting with "colornewst.PID" and removed at the end, if there is an error, or if the program is interrupted with Ctrl-C. If the output file exists, it is turned into a backup file by appending "~" to the name unless the environment variable IMOD_NO_IMAGE_BACKUP is set. AUTHOR David Mastronarde SEE ALSO newstack, clip, tif2mrc, mrc2tif BUGS Email bug reports to mast at colorado dot edu. IMOD 4.11.0 colornewst(1)