clipmodel(1) General Commands Manual clipmodel(1) NAME clipmodel - to clip a MT model by location or object number SYNOPSIS clipmodel [options] input_file output_file DESCRIPTION Clipmodel will clip out a portion of a model and output either a new model file, or a simple list of point coordinates. It can either include only points within a defined rectangular block of 3- dimen- sional coordinate space, or exclude points within such a block. This process of inclusion or exclusion may be invoked repeatedly. The pro- gram can also eliminate an entire IMOD object. To specify a rectangular block of coordinate space, one enters the min- imum and maximum X, Y and Z coordinates of the block. The default val- ues for the minimum and maximum X and Y coordinates are very large num- bers, so one can select the default if one wants no limitation on either the X, the Y, or both coordinates. A point on the edge of the block (i.e. with one coordinate exactly equal to one of the lower or upper limits) is treated as if it is inside the block. The Z limits are extended by 0.005 to help this work as expected even if Z values differ slightly from integers; for example, limits of 117,117 will retain the point at Z = 117. The coordinates that you enter should correspond to the index coordi- nates in the image display from which the model was last saved. If you last saved the model from an 3dmod display of a subset of the image stack, then you need to refer to coordinates within that subset. If this is a problem, reload the model onto the full image stack (or a subset starting at 0,0,0) and save it again. Index coordinates are num- bered from zero, so your Z values should be 1 less than the section number shown in 3dmod. The program does not create new contours. This limitation means that if there is more than one segment of a contour inside a block being included (or more than one segment outside a block being excluded), you have two options. The program either can truncate the contour to be the longest such segment, discarding any other segments, or it can clip all of the points out of the contour that are in the region being excluded, connecting the remaining segments with straight lines. If an object consists of scattered points, then all points in the region being included will be included, regardless of which clipping option you choose for clipping contours. When you choose to retain all segments in the region being included, the program will sum and report the total length of cut edges, so that the cut surface can be subtracted from a computed surface area. For objects consisting of closed contours, this total will include an edge between the starting and ending points of a clipped contour, if points were clipped off at those ends of the contour. For open contour objects, this edge will not be included. OPTIONS Clipmodel uses the PIP package for input (see the manual page for pip) and can still take input interactively if a sequence of opera- tions is needed that cannot be achieved with the PIP options. The fol- lowing options can be specified either as command line arguments (with the -) or one per line in a command file or parameter file (without the -). Options can be abbreviated to unique letters; the currently valid abbreviations for short names are shown in parentheses. -input (-i) OR -InputFile File name Input model file to be clipped -output (-ou) OR -OutputFile File name Name of output file, which can be either an IMOD model file or text file with a list of point coordinates. -point (-po) OR -PointOutput Integer Enter 1 to make a point output file instead of a model output file. Nearest integer coordinates will be output; use Model2point to get more flexible point list output. Enter -1 to get a point list with corner points added each time a new Z value is encountered; in this case -xminmax and -yminmax must be entered. -xminmax (-x) OR -XMinAndMax Two floats Starting and ending X index coordinates to include or exclude, numbered from 0. Enter a / to take the default, which is to have no selection based on X coordinate. (Successive entries accumulate) -yminmax (-y) OR -YMinAndMax Two floats Starting and ending Y index coordinates to include or exclude, numbered from 0. Enter a / to take the default, which is to have no selection based on Y coordinate. (Successive entries accumulate) -zminmax (-z) OR -ZMinAndMax Two floats Starting and ending Z coordinates to include or exclude, num- bered from 0. Enter a / to take the default, which is to have no selection based on Z coordinate. Starting and ending will be adjusted down and up by 0.005, respectively, so whole numbers can be entered; e.g., 16,18 will clip out sections 16 through 18. (Successive entries accumulate) -bound (-b) OR -BoundaryObjects List of integer ranges List of model objects with contours enclosing a volume to include or exclude. If coordinate limits are also entered, then the intersection of the regions inside those limits and the interior of the contours will be used as the volume to include or exclude. Objects must be closed contour objects. There can be more than one contour on each section, and contours do not need to be drawn on every section included in the volume. Points are tested for whether they are inside the boundary con- tours on the nearest section with such contours, unless the -coplanar option is given. Points beyond the minimum and maxi- mum Z values of the boundary contours are considered outside the volume. The listed objects will be excluded from the clipping operations; not even coordinate limits are applied to them. If multiple operations are specified, the boundary contours are applied only on the first operation. -coplanar (-c) OR -CoplanarBoundary Boundary contours apply only on the planes where they are drawn; thus, to be considered inside the volume, a point must be on the same plane as, and inside, some boundary contour. -exclude (-ex) OR -ExcludeOrInclude Multiple integers 1 to exclude or 0 to include points within specified coordinate block. Multiple values can be entered for a sequence of opera- tions. The number of -xminmax, -yminmax, and -zminmax entries must each be equal to the number of values entered here, unless an option is not entered at all for that dimension. If any coordinate limits are entered, the default for this entry is a single 0; in other words, you can omit this entry if you are just clipping out coordinates inside one coordinate block. -longest (-l) OR -LongestContourSegment Retain only the longest segment of a contour that is within the volume being included, instead of all segments. -keep (-k) OR -KeepEmptyContours Keep contours that have no points, so that contours have their original numbers in the output model. This was the behavior before IMOD 4.6.18. The default is now to remove empty con- tours. -objects (-ob) OR -ObjectList List of integer ranges List of model objects to remove or clip endpoints from. This option cannot be entered together with any of the options for clipping by coordinates. If -ends is entered, points will be removed from endpoints of contours; otherwise whole objects will be removed. -ends (-en) OR -ClipFromStartAndEnd Two integers Number of points to clip from start and end of contours for the objects specified by -objects; the latter must be entered. -values (-v) OR -ValuesInOrOutOfRange Integer Use this option to delete contours or points that have stored values inside of or outside of a defined range. Enter 1 or -1 to delete contours, 2 or -2 to delete points, or 3 or -3 to delete both, with a positive or negative number to delete if values are inside or outside the range, respectively. For exam- ple, if a model is produced by Imodfindbeads with the default storage option, contours with values below threshold can be removed with "clipmodel -values 1 input_file output_file". -range (-r) OR -RangeForValues Two floats When deleting points or contours with the -values option, use this option to enter the lower and upper values of the range used to decide whether a point or contour is to be deleted. If this option is not entered, the default range for each object is all values below the lower threshold stored in the model by Imodfindbeads, Findbeads3d, 3dmod, or other programs. In 3dmod, this threshold appears in the Bead Fixer dialog and also as the Black slider setting in the Values panel of the Edit-Objects dialog of the Model View window. When deleting closed contours by area with the -areas option, use this option to enter the lower and upper areas of the range. -update (-up) OR -UpdateObjectMinMax Update the stored minimum and maximum values of each object based on the stored values of all contours and points in the object. These minimum and maximum values determine the range of the Black/White sliders in the Values panel of the Edit-Objects dialog of the Model View window. This option cannot be used together with -keep. -areas (-a) OR -AreasInOrOutOfRange Integer Use this option to delete contours in closed contour objects with areas inside or outside of a range specified with the -range option, which must be entered as well. Enter a positive value to delete inside the range or a negative one to delete outside the range. Enter -1 or 1 if the area limits entered with -fBrange are in pixels, -2 or +2 if they are in square nanometers, or -3 or 3 if they are in square microns. This option cannot be entered with -values. -param (-pa) OR -ParameterFile Parameter file Read parameter entries as keyword-value pairs from a parameter file. -help (-h) OR -usage Print help output -StandardInput Read parameter entries from standard input. INTERACTIVE INPUT In interactive mode, entries to the program are: Name of input model file Name of output file for clipped model 0 to output a model file, 1 to output a file with a simple list of integer point coordinates, or -1 to output such a point list with two additional corner points preceding the points on any given section. Such a file could be used as a set of teaching points for MTTEACH and MTDETECT; but beware of the need for each "zone" to be contained within a single piece in a montaged image file. 0 to retain only the longest segment of a contour included in a volume (if the contour passes through the volume more than once), or 1 to retain all segments included in the volume. 0 to include points in the rectangular coordinate block to be specified next, or 1 to exclude points in such a block, or -1 to exclude whole IMOD objects, or -2 to clip points off of one end of all contours in selected IMOD objects. IF you entered 0 or 1, next specify the coordinates as follows: Minimum and maximum X, minimum and maximum Y coordinates of the block. / selects the defaults of no limits Minimum and maximum Z of the block BUT IF you entered -1, enter instead a list of the numbers of the objects to be excluded. For data from a WIMP model file, use 256 minus the WIMP object color (e.g. 1 for color 255), or the negative of this value to refer to WIMP objects that are turned off. Ranges may be entered (e.g. 1-3,6). OR IF you entered -2, enter the number of points to clip off of the start, and the number of points to clip off of the end, of contours in selected objects. Next enter a list of the numbers of the objects to be clipped in this fashion, where WIMP objects may again be referred to by 256 minus their color, or the negative of this value if they are turned off. Ranges may be entered. 0 to store the current result, or 1 to loop back and specify a new block to include or exclude (or objects to exclude or clip) HISTORY Written by David Mastronarde, 1/11/90 Modified for IMOD 4/24/97 Compute cut edges and handle coordinates better, 10/24/00 Converted to PIP input, July 2012. BUGS Email bug reports to mast at colorado dot edu. IMOD 4.11.0 clipmodel(1)