avganova(1) General Commands Manual avganova(1)NAMEavganova - compare images using nested analysis of varianceSYNOPSISavganovaDESCRIPTIONAvganova will do statistical comparisons, using nested analysis of variance, on the output of the program Imavgstat(10. This output consists of mean, standard deviation, and standard error of the mean for all of the summing areas in a series of different data sets. The summing areas were derived from a set of summing regions specified by an IMOD model; each summing region was divided into one or more summing areas. To set up a comparison, you designate one collection of data sets as Group 1, and another collection of data sets as Group 2. (The ANOVA requires that each group contain more than one data set.) You then specify a collection of summing regions. The ANOVA will be run sepa- rately on each summing area within those regions. More generally, you can do a multiple comparison of differences among more than 2 Groups. Each of the data sets included in a comparison may be rescaled indepen- dently; i.e. a particular linear scaling may be applied to all of the areas in a data set, a different scaling may be applied to all areas in another set, etc. It is also possible to apply the same scaling, or the same form of scaling, to all data sets without entering values for each set separately. Scaling may be specified in four ways: 1) One may directly specify a factor to multiply by and a factor to add. 4) One may specify that the values for a set are all to be divided by the value for a specified area of that set. 3) One may specify that a given set should have its values shifted (without any multiplication) so that the mean of a particular collection of summing regions matches the corresponding mean for some other data set. 4) One may do a least- squares linear regression between the data points of the set being scaled and the corresponding data points of some other set, and use the coefficients of the regression to determine the scaling factors. The data points used for regression are the means from the summing areas within a particular collection of summing regions. Entries to the program are now described in order as they are first encountered. After doing one comparison, one may loop back to a vari- ety of different points in order to change different parameters. Name of statistics file output by Imavgstat Number of groups of sets List of numbers of the sets to include in Group 1. Sets are numbered from 1. You can enter ranges separated by commas, e.g. 1-3,7-9 List of numbers of the sets to include in Group 2. List of numbers of the sets to include in Group 3, if any, etc. List of numbers of the regions to compare. Ranges are OK 0 to compare the means of the summing areas, or 1 to compare the integrals, which are the means times the number of pixels. List of numbers of sets to rescale - ranges may be entered, or just Return for no rescaling, or enter / to select either all sets or the sets selected last time, as indicated by the prompt. IF you select rescaling, first enter 0 to specify scaling separately for each set, or 1 to apply the similar scaling to all sets. IF you select rescaling, next make the following entries for each set that you specified for rescaling: 0 to specify scaling factors directly, 999 to divide values by the value in one area, or the number of another data set, if you wish to regress this set against the other set, or the negative of the number of another set, if you wish to shift this set to have the same mean as that set. IF you entered 0, next enter the factor to multiply by, and the amount to add after multiplication IF you entered 999, next enter the region number, and the number of the area within that region, to divide by. BUT, IF you entered a set number, next enter a list of the numbers of the regions to use for comparing the two data sets. For each comparison, the program first prints a t-statistic (with sig- nificance level) for a simple comparison of the mean of the means in Groups 1 and 2. This statistic is not as valid as the one from the ANOVA which follows. However, it is a one-tailed statistic and might be more suitable for evaluating whether the two groups differ in a direction that was expected a priori. The top line of the ANOVA table shows the significance of difference between the two groups. The sec- ond line shows the significance of differences among the different data sets within each group (subgroup differences). If the conditions for the Satterthwaite approximation are satisfied, then the results from that approximation are printed next and should be used for comparison between groups, instead of the first line of the table. After the comparisons, enter one of the following: 1 to loop back to the specification of rescaling of sets 2 to loop back to entering the list of regions to compare 3 to loop back to entering the list of data sets in the groups 4 to loop all the way back and read a new data file 5 to exitHISTORYWritten by David Mastronarde, 4/23/90BUGSEmail bug reports to mast at colorado dot edu. IMOD 4.10.15 avganova(1)