Dissemination
One of the laboratory's missions as a national resource facility is to disseminate information on techniques and software that have been developed and advanced here. Below are a list of documents containing instructions, strategies, and procedures for sample preparation and for the use of the software packages that have been developed for tomographic acquisition. The following links are to pdf documents.
Sample Preparation
The Boulder Laboratory for 3D Electron Microscopy developes techniques for preserving cellular structure with the highest degree of reliability. These techniques involve different methods for rapidly freezing samples in order to halt structural and biochemical activity in a very short timeframe, thus preserving structure in the "live" state.
Manual
Practical Methods in High-Pressure Freezing, Freeze-Substitution, Embedding and Immunoctocheminstry for Electron Microscopy
This manual is a technical guide to methods in high pressure freezing and subsequent freeze-substitution fixation and embedding for high resolution electron microscopy and EMimmunocytochemistry.
Software Documentation
Visit the IMOD and SerialEM websites for complete documentation and software downloads. Below is a list of quick links to some of the information on these sites.
IMOD
IMOD User Guide
Introduction to 3DMOD
Tomography Guide
Guide to joining plastic serial section tomograms
SerialEM
SerialEM help documents
Introduction to SerialEM
A detailed introduction into the basic operations of Serial EM
Low dose, Navigator, Energy Filtering
Overview of low dose mode, using the navigator and energy filtering
Maps and Low Dose
How to set up medium and low magnification maps, and setting up low dose
Stategies for Cryo-Tomography Tilts Series Acquisition
Addresses the challanges inherent in cryo electron tomography image acquisition
Macros, Camera issues, Setting up the program
Using macros, camera issues, and setting up Serial EM
Optomizing Tomography for Plastic Sections
Special considerations for working with plastic embedded sections
