One of the laboratory's missions as a national resource facility is to disseminate information on techniques and software that have been developed and advanced here. Below are a list of documents containing instructions, strategies, and procedures for sample preparation and for the use of the software packages that have been developed for tomographic acquisition. The following links are to pdf documents.
The Boulder Laboratory for 3D Electron Microscopy developes techniques for preserving cellular structure with the highest degree of reliability. These techniques involve different methods for rapidly freezing samples in order to halt structural and biochemical activity in a very short timeframe, thus preserving structure in the "live" state.
Practical Methods in High-Pressure Freezing, Freeze-Substitution, Embedding and Immunoctocheminstry for Electron Microscopy
This manual is a technical guide to methods in high pressure freezing and subsequent freeze-substitution fixation and embedding for high resolution electron microscopy and EMimmunocytochemistry.
Visit the IMOD, PEET, and SerialEM websites for complete documentation and software downloads. Below is a list of quick links to some of the information on these sites. Tutorial videos are also available on our YouTube channel.
A detailed introduction into the basic operations of Serial EM
Overview of low dose mode, using the navigator and energy filtering
How to set up medium and low magnification maps, and setting up low dose
Addresses the challanges inherent in cryo electron tomography image acquisition
Using macros, camera issues, and setting up Serial EM
Special considerations for working with plastic embedded sections