Multiple Record Setup Dialog
This dialog allows you to set parameters for taking multiple Record images in a circular pattern around the current position, with image shift used to center on each position. The parameters are used in two ways: when acquiring the images with the 'MultipleRecords' script command, and when displaying the positions as a pattern of circles representing the beam on an image (e.g., a lower-magnification map). The latter display occurs when the 'Show Acquire area' checkbox in the Navigator dialog is turned on, provided that the display is enabled with the Show Shots when Show Acquire option in the Navigator - Montaging & Grids submenu. When this display is active, changes in the relevant parameters here will appear immediately in display.
Number of shots around center
Select a value between 2 and 12 for the number of Records taken in off-center positions.
Shot in center
With these radio buttons, you can include a shot in the center of the pattern, which should be possible with 6 or more off-center shots. The centered shot can be taken either before or after the off-center shots. Although taking it may involve one more delay for image shift settling, it is reported that this shot will have less drift when take after the others.
Distance from center to off-center shots
Distance from the center position to the off-center positions, in microns. This distance must be at least as big as the beam diameter to avoid exposing overlapping areas in more than one Record shot. If the multi-shot circles are being displayed, press tab or Enter to have the image redrawn after entering a new distance.
Early returns for K2
This group of controls appears when there is a K2 camera available and allows for SerialEM to resume operations as soon as possible after acquiring an image with saved frames, the same as is accomplished with the script command 'EarlyReturnNextShot'. The radio buttons allow you to select to have an early return only on the last shot, or on all shots. If post-exposure actions are enabled for the camera (namely, if Post-actions in the Camera menu is not grayed out and is selected), then image shift to the next position will occur soon after the exposure is finished for each shot, regardless of whether early return is selected, and it is slightly more efficient not to do an early return except on the last shot. If post-actions are not enabled, the early return will allow the program to do the image shift sooner.
In the text box in this group, enter the number of frames in the returned image: 0 for none, a specific number of frames, or -1 for all the frames.
Save Record image
Select this option to have the Record image saved in the current file. The option is disabled and its setting is irrelevant if an early return is selected with no returned image.
Extra delay after image shift
A standard amount of settling time is imposed after each image shift; the extra delay entered here will be added to this settling time.
Diameter of circles to draw
If the multi-shot circles are being displayed, the diameter of the circles is set to the entered value in microns, unless the option described next is selected to use the illuminated area on an FEI Titan. Press tab or Enter to have the image redrawn after entering a new diameter.
Use Record illuminated area for circles in Low Dose
This option appears on an FEI Titan and allows the multi-shot circle display to take its diameter from the illuminated area setting of the Record area when Low Dose mode is on instead of from the value entered in this dialog. The display will be updated dynamically as this illuminated area setting is changed. This update works even while this dialog is option, provided that the scope is in the Record area and 'Continuous update' is check in the Low Dose Control panel before opening the dialog, and illuminated area is changed with microscope controls.
For this option to be valuable rather than misleading, the illuminated area readout must be fairly accurate in the range of interest, which may not be the case.