Camera & Script Control Panel

This panel has buttons for taking images and controlling scripts or actions during a tilt series. Several of the buttons will change when the Tilt Series Controller is activated and when data are being acquired at points through the Navigator.  The three script buttons can be used not only to run the selected script but also to open an editor for an existing script, by holding down the Ctrl key while clicking on the button.

Setup

This button will open the Camera Setup dialog box and allow you to set parameters like exposure time, field of view and binning for the 5 different exposure modes

View

Focus

Trial

Record

These buttons will acquire an image using the View, Focus, Trial, or Record parameters.

Preview/Montage

Ordinarily, this button will acquire an image with the Preview parameter set. If montaging has been started, the button will acquire a montaged image.

End

This button has several uses for ending an operation in an orderly fashion, and its label sometimes changes accordingly. If a script is running, this button will end the script at the end of a loop or at the end of the script. If the Tilt Series Controller is active, the button will stop the series after completing all actions at the current tilt angle. If a script or tilt series was being run from the Navigator and was stopped, this button can also be used to terminate the script at the current position, or terminate the tilt series, and allow the Navigator to go on to the next position. Otherwise, when the Navigator is acquiring data, the button will signal the Navigator to end its acquisitions after finishing the current item.

Resume

If a script has been running, this button will resume the script at the next step. If the Tilt Series Controller is active, the button will open the Tilt Series Resume dialog box

STOP

This is a general Stop button for image acquisition, autofocus, montaging, scripts, tasks, tilt series, or any other extended sequence of actions. It will generally stop the activity as soon as possible after the current action. When you are running a tilt series or a script from the Navigator, the Stop button acts on the lowest level of operation only.

Script/One Loop

If the Tilt Series Controller is not active, this button will execute the script shown on the button; otherwise it will execute one loop of the tilt series. The program will stop at the same place where the End button would stop it, after finishing all actions at a tilt angle.

Script/One Step

If the Tilt Series Controller is not active, this button will execute the script shown on the button; otherwise it will advance one step of the tilt series. There are many more steps in the loop of the Tilt Series Controller than actual actions under particular conditions, but it should generally stop after the next actual action.

Script/Back Up

If the Tilt Series Controller is not active, this button will execute the script shown on the button; otherwise it will open the Tilt Series Back Up dialog box

Script Selector Spin Buttons

These spin buttons will change the script number activated by the associated Script button.