*** Entries to program tilt *** InputProjections = WTI042413_1series4_3dfind.ali OutputFile = WTI042413_1series4_erase.fid ProjectModel = WTI042413_1series4_3dfind.mod ActionIfGPUFails = 1,2 AdjustOrigin = 1 FULLIMAGE = 1852 1856 IMAGEBINNED = 3 LOG = 0.0 MODE = 1 OFFSET = 0.0 PERPENDICULAR = 1 RADIAL = 0.35 0.05 SCALE = 0.0 700.0 SHIFT = 0.0 9.1 SUBSETSTART = -1 -2 THICKNESS = 350 TILTFILE = WTI042413_1series4.tlt XAXISTILT = 1.65 XTILTFILE = WTI042413_1series4.xtilt *** End of entries *** RO image file on unit 1 : WTI042413_1series4_3dfind.ali Size= 45651 K Number of columns, rows, sections ..... 618 620 61 Map mode .............................. 1 (16-bit integer) Start cols, rows, sects, grid x,y,z ... 0 0 0 618 620 61 Pixel spacing (Angstroms).............. 20.22 20.22 6.740 Cell angles ........................... 90.000 90.000 90.000 Fast, medium, slow axes ............... X Y Z Origin on x,y,z ....................... 6.740 13.48 0.000 Minimum density ....................... -32.245 Maximum density ....................... 734.46 Mean density .......................... 223.79 tilt angles (original,current) ........ 0.0 0.0 0.0 0.0 0.0 0.0 Space group,# extra bytes,idtype,lens . 0 854 0 0 4 Titles : SerialEM: Digitized on Janelia Krios 26-Apr-13 14:20:21 Tilt axis angle = 168.0, binning = 2 spot = 7 camera = 3 CCDERASER: Bad points replaced with interpolated values 26-Apr-13 17:17:47 NEWSTACK: Images copied, transformed 28-May-13 13:06:38 Radial weighting function parameters IRMAX = 216 IWIDE = 30 Output map densities incremented by 0.00 and then multiplied by 700.00 Output map is sectioned perpendicular to the tilt axis Data mode of output file is 1 Taking logarithm of input data plus 0.000 FORMATTED ro file opened: /home/cschwart/JaneliaFarms/WTI042413_1/series4/WTI042413_1series4.tlt FORMATTED ro file opened: /home/cschwart/JaneliaFarms/WTI042413_1/series4/WTI042413_1series4.xtilt Constant alpha tilt of 0.0 to be applied based on angles from file Global alpha tilt of 1.6 will be applied Dimensions and coordinates will be scaled down by a factor of 3 ------------------------------------------------------------------------------ Thickness of reconstructed slice is 116 pixels. Full aligned stack will be assumed to be 618 by 619 pixels Aligned stack will be assumed to be a subset starting at 0 0 Output slice shifted up 3.0 and to right 0.0 pixels Tilt angles were entered from a tilt file Projection angles: -58.94 -56.94 -54.92 -52.91 -50.92 -48.92 -46.93 -44.92 -42.91 -40.90 -38.89 -36.89 -34.90 -32.91 -30.92 -28.93 -26.94 -24.94 -22.93 -20.93 -18.93 -16.93 -14.93 -12.93 -10.92 -8.92 -6.92 -4.91 -3.02 -1.12 0.78 2.68 4.58 6.48 8.52 10.56 12.60 14.64 16.68 18.72 20.72 22.72 24.73 26.73 28.73 30.77 32.80 34.84 36.87 38.90 40.94 42.97 45.00 47.03 49.06 51.03 53.00 54.96 57.01 59.06 61.11 ------------------------------------------------------------------------------ 9333 points written to output model SUCCESSFULLY COMPLETED