*** Entries to program tilt *** InputProjections = WTI042413_1series4_3dfind.ali OutputFile = WTI042413_1series4_3dfind.rec ActionIfGPUFails = 2,2 AdjustOrigin = 1 FULLIMAGE = 1852 1856 IMAGEBINNED = 3 LOG = 0.0 MODE = 1 OFFSET = 0.0 PERPENDICULAR = 1 RADIAL = 0.35 0.05 SCALE = 0.0 700.0 SHIFT = 0.0 9.1 SLICE = -1 -1 SUBSETSTART = -1 -2 THICKNESS = 350 TILTFILE = WTI042413_1series4.tlt TOTALSLICES = 0 1857 XAXISTILT = 1.65 XTILTFILE = WTI042413_1series4.xtilt *** End of entries *** RO image file on unit 1 : WTI042413_1series4_3dfind.ali Size= 45651 K Number of columns, rows, sections ..... 618 620 61 Map mode .............................. 1 (16-bit integer) Start cols, rows, sects, grid x,y,z ... 0 0 0 618 620 61 Pixel spacing (Angstroms).............. 20.22 20.22 6.740 Cell angles ........................... 90.000 90.000 90.000 Fast, medium, slow axes ............... X Y Z Origin on x,y,z ....................... 6.740 13.48 0.000 Minimum density ....................... -32.245 Maximum density ....................... 734.46 Mean density .......................... 223.79 tilt angles (original,current) ........ 0.0 0.0 0.0 0.0 0.0 0.0 Space group,# extra bytes,idtype,lens . 0 854 0 0 4 Titles : SerialEM: Digitized on Janelia Krios 26-Apr-13 14:20:21 Tilt axis angle = 168.0, binning = 2 spot = 7 camera = 3 CCDERASER: Bad points replaced with interpolated values 26-Apr-13 17:17:47 NEWSTACK: Images copied, transformed 28-May-13 13:06:38 Radial weighting function parameters IRMAX = 216 IWIDE = 30 Output map densities incremented by 0.00 and then multiplied by 700.00 Output map is sectioned perpendicular to the tilt axis Data mode of output file is 1 Taking logarithm of input data plus 0.000 FORMATTED ro file opened: /home/cschwart/JaneliaFarms/WTI042413_1/series4/WTI042413_1series4.tlt FORMATTED ro file opened: /home/cschwart/JaneliaFarms/WTI042413_1/series4/WTI042413_1series4.xtilt Constant alpha tilt of 0.0 to be applied based on angles from file Global alpha tilt of 1.6 will be applied Dimensions and coordinates will be scaled down by a factor of 3 ------------------------------------------------------------------------------ Rows 0 to 0, (at intervals of 1) of the projection planes will be reconstructed. Thickness of reconstructed slice is 116 pixels. Full aligned stack will be assumed to be 618 by 619 pixels Aligned stack will be assumed to be a subset starting at 0 0 Output slice shifted up 3.0 and to right 0.0 pixels Computed slices are part of a total volume from slice 1 to 620 Tilt angles were entered from a tilt file NEW image file on unit 2 : WTI042413_1series4_3dfind.rec Projection angles: -58.94 -56.94 -54.92 -52.91 -50.92 -48.92 -46.93 -44.92 -42.91 -40.90 -38.89 -36.89 -34.90 -32.91 -30.92 -28.93 -26.94 -24.94 -22.93 -20.93 -18.93 -16.93 -14.93 -12.93 -10.92 -8.92 -6.92 -4.91 -3.02 -1.12 0.78 2.68 4.58 6.48 8.52 10.56 12.60 14.64 16.68 18.72 20.72 22.72 24.73 26.73 28.73 30.77 32.80 34.84 36.87 38.90 40.94 42.97 45.00 47.03 49.06 51.03 53.00 54.96 57.01 59.06 61.11 ------------------------------------------------------------------------------ Exiting after setting up output file for chunk writing SUCCESSFULLY COMPLETED CHUNK DONE