*** Entries to program tilt *** InputProjections = WTI042413_1series4.ali OutputFile = WTI042413_1series4_full.rec ActionIfGPUFails = 2,2 AdjustOrigin = 1 FULLIMAGE = 1852 1856 IMAGEBINNED = 1 MODE = 1 OFFSET = 0.0 PERPENDICULAR = 1 RADIAL = 0.35 0.05 SCALE = 0.0 0.1 SHIFT = 0.0 0.0 SLICE = -1 -1 SUBSETSTART = 0 0 THICKNESS = 266 TILTFILE = WTI042413_1series4.tlt TOTALSLICES = 0 1855 UseGPU = 0 XAXISTILT = 1.65 XTILTFILE = WTI042413_1series4.xtilt *** End of entries *** RO image file on unit 1 : WTI042413_1series4.ali Size= 409524 K Number of columns, rows, sections ..... 1852 1856 61 Map mode .............................. 1 (16-bit integer) Start cols, rows, sects, grid x,y,z ... 0 0 0 1852 1856 61 Pixel spacing (Angstroms).............. 6.740 6.740 6.740 Cell angles ........................... 90.000 90.000 90.000 Fast, medium, slow axes ............... X Y Z Origin on x,y,z ....................... 0.000 0.000 0.000 Minimum density ....................... -250.00 Maximum density ....................... 886.00 Mean density .......................... 223.66 tilt angles (original,current) ........ 0.0 0.0 0.0 0.0 0.0 0.0 Space group,# extra bytes,idtype,lens . 0 0 0 0 6 Titles : SerialEM: Digitized on Janelia Krios 26-Apr-13 14:20:21 Tilt axis angle = 168.0, binning = 2 spot = 7 camera = 3 CCDERASER: Bad points replaced with interpolated values 26-Apr-13 17:17:47 NEWSTACK: Images copied, undistorted 28-May-13 11:02:55 ctfPhaseFlip: CTF correction with phase flipping only 28-May-13 11:04:03 CCDERASER: Bad points replaced with interpolated values 28-May-13 13:09:31 Radial weighting function parameters IRMAX = 648 IWIDE = 92 Output map densities incremented by 0.00 and then multiplied by 0.10 Output map is sectioned perpendicular to the tilt axis Data mode of output file is 1 FORMATTED ro file opened: /home/cschwart/JaneliaFarms/WTI042413_1/series4/WTI042413_1series4.tlt FORMATTED ro file opened: /home/cschwart/JaneliaFarms/WTI042413_1/series4/WTI042413_1series4.xtilt Constant alpha tilt of 0.0 to be applied based on angles from file Global alpha tilt of 1.6 will be applied ------------------------------------------------------------------------------ Rows 0 to 0, (at intervals of 1) of the projection planes will be reconstructed. Thickness of reconstructed slice is 266 pixels. Full aligned stack will be assumed to be 1852 by 1856 pixels Aligned stack will be assumed to be a subset starting at 0 0 Computed slices are part of a total volume from slice 1 to 1856 Tilt angles were entered from a tilt file NEW image file on unit 2 : WTI042413_1series4_full.rec Projection angles: -58.94 -56.94 -54.92 -52.91 -50.92 -48.92 -46.93 -44.92 -42.91 -40.90 -38.89 -36.89 -34.90 -32.91 -30.92 -28.93 -26.94 -24.94 -22.93 -20.93 -18.93 -16.93 -14.93 -12.93 -10.92 -8.92 -6.92 -4.91 -3.02 -1.12 0.78 2.68 4.58 6.48 8.52 10.56 12.60 14.64 16.68 18.72 20.72 22.72 24.73 26.73 28.73 30.77 32.80 34.84 36.87 38.90 40.94 42.97 45.00 47.03 49.06 51.03 53.00 54.96 57.01 59.06 61.11 ------------------------------------------------------------------------------ Exiting after setting up output file for chunk writing SUCCESSFULLY COMPLETED CHUNK DONE