Enter 0 to align all sections to a single average central alignment; or 1 to align to an average alignment that shifts based on a polynomial fit to the whole stack; or N to align each section to an average alignment based on a polynomial fit to the nearest N sections, or -1 or -N for a hybrid of central and shifting alignments: Input file of f transforms: Output file of g transforms: FORMATTED old file opened: /home/cschwart/JaneliaFarms/WTI042413_1/series4/WTI042413_1series4.prexf FORMATTED new file opened: /home/cschwart/JaneliaFarms/WTI042413_1/series4/WTI042413_1series4.prexg *** Entries to program newstack *** InputFile = WTI042413_1series4.st OutputFile = WTI042413_1series4.preali TransformFile = WTI042413_1series4.prexg DistortionField = 33kGIF_nos.idf ImagesAreBinned = 2 ModeToOutput = 0 FloatDensities = 2 *** End of entries *** FORMATTED ro file opened: /home/cschwart/JaneliaFarms/WTI042413_1/series4/WTI042413_1series4.prexg RO image file on unit 1 : WTI042413_1series4.st Size= 409529 K Number of columns, rows, sections ..... 1852 1856 61 Map mode .............................. 1 (16-bit integer) Start cols, rows, sects, grid x,y,z ... 0 0 0 1852 1856 61 Pixel spacing (Angstroms).............. 6.740 6.740 6.740 Cell angles ........................... 90.000 90.000 90.000 Fast, medium, slow axes ............... X Y Z Origin on x,y,z ....................... 0.000 0.000 0.000 Minimum density ....................... -96.000 Maximum density ....................... 1021.0 Mean density .......................... 223.88 tilt angles (original,current) ........ 0.0 0.0 0.0 0.0 0.0 0.0 Space group,# extra bytes,idtype,lens . 0 5120 0 0 3 Titles : SerialEM: Digitized on Janelia Krios 26-Apr-13 14:20:21 Tilt axis angle = 168.0, binning = 2 spot = 7 camera = 3 CCDERASER: Bad points replaced with interpolated values 26-Apr-13 17:17:47 NEW image file on unit 2 : WTI042413_1series4.preali section input min&max output min&max & mean 0 -80.02 962.27 37.41 179.67 81.74 1 -57.62 896.81 38.18 179.06 81.74 2 -69.52 882.11 33.76 183.65 81.74 3 -48.47 850.12 33.24 183.24 81.74 4 -75.92 875.44 27.46 195.88 81.74 5 -51.25 793.60 27.04 189.37 81.74 6 -52.42 799.35 27.03 193.16 81.74 7 -68.98 768.38 21.24 193.92 81.74 8 -93.13 800.44 14.07 204.99 81.74 9 -46.67 746.99 22.04 197.73 81.74 10 -52.73 739.46 18.26 199.59 81.74 11 -68.88 785.68 13.21 216.09 81.74 12 -45.95 729.85 12.52 209.23 81.74 13 -46.91 721.17 14.40 212.19 81.74 14 -45.78 716.40 14.31 210.23 81.74 15 -81.84 670.68 3.22 202.41 81.74 16 -64.38 743.07 6.51 225.59 81.74 17 -99.81 698.34 0.00 218.54 85.08 18 -47.67 691.13 8.38 219.94 81.74 19 -53.03 665.26 5.92 214.93 81.74 20 -47.60 664.34 6.53 216.70 81.74 21 -46.55 621.12 5.85 207.61 81.74 22 -45.95 658.83 5.15 220.56 81.74 23 -57.74 676.82 0.03 230.50 81.74 24 -45.99 654.52 3.47 224.63 81.74 25 -39.64 603.26 0.27 210.46 81.74 26 -42.82 654.86 4.02 225.09 81.74 27 -53.65 646.50 0.00 227.12 82.54 28 -54.37 661.61 0.00 230.20 82.24 29 -44.27 651.18 0.00 227.11 81.83 30 -48.39 628.88 1.06 219.17 81.74 31 -50.01 676.35 0.18 233.12 81.74 32 -43.23 613.23 0.00 216.53 82.45 33 -44.05 683.07 1.62 237.79 81.74 34 -54.32 686.06 0.00 235.26 82.15 35 -41.42 612.25 3.63 210.62 81.74 36 -69.84 655.10 0.00 222.66 84.75 37 -71.40 630.97 0.00 216.72 85.61 38 -60.07 631.42 0.00 212.65 82.53 39 -49.67 659.52 2.57 219.28 81.74 40 -44.47 709.39 4.83 231.27 81.74 41 -39.49 660.29 6.54 215.08 81.74 42 -71.34 713.59 0.00 226.21 82.80 43 -49.44 673.76 5.40 211.85 81.74 44 -45.29 689.28 7.18 213.64 81.74 45 -58.80 706.05 4.72 213.43 81.74 46 -52.11 756.40 7.50 223.16 81.74 47 -53.50 715.75 8.11 209.88 81.74 48 -54.07 747.40 9.52 212.10 81.74 49 -69.89 764.62 7.01 212.36 81.74 50 -43.13 882.96 13.49 238.25 81.74 51 -46.75 757.01 14.97 200.79 81.74 52 -50.94 787.68 16.53 202.39 81.74 53 -51.43 782.16 17.95 195.47 81.74 54 -49.94 888.23 17.38 213.06 81.74 55 -78.58 819.05 16.42 195.47 81.74 56 -68.52 947.59 20.46 210.54 81.74 57 -49.13 921.43 26.32 197.02 81.74 58 -46.12 909.63 27.43 188.88 81.74 59 -91.49 968.96 22.75 189.97 81.74 60 -65.50 985.65 30.84 183.73 81.74 SUCCESSFULLY COMPLETED