*** Entries to program newstack *** InputFile = WTI042413_1series4.st OutputFile = WTI042413_1series4.ali OffsetsInXandY = 0.0,0.0 TransformFile = WTI042413_1series4.xf BinByFactor = 1 DistortionField = 33kGIF_nos.idf ImagesAreBinned = 2 AdjustOrigin = 1 TaperAtFill = 1,0 *** End of entries *** FORMATTED ro file opened: /home/cschwart/JaneliaFarms/WTI042413_1/series4/WTI042413_1series4.xf RO image file on unit 1 : WTI042413_1series4.st Size= 409529 K Number of columns, rows, sections ..... 1852 1856 61 Map mode .............................. 1 (16-bit integer) Start cols, rows, sects, grid x,y,z ... 0 0 0 1852 1856 61 Pixel spacing (Angstroms).............. 6.740 6.740 6.740 Cell angles ........................... 90.000 90.000 90.000 Fast, medium, slow axes ............... X Y Z Origin on x,y,z ....................... 0.000 0.000 0.000 Minimum density ....................... -96.000 Maximum density ....................... 1021.0 Mean density .......................... 223.88 tilt angles (original,current) ........ 0.0 0.0 0.0 0.0 0.0 0.0 Space group,# extra bytes,idtype,lens . 0 5120 0 0 3 Titles : SerialEM: Digitized on Janelia Krios 26-Apr-13 14:20:21 Tilt axis angle = 168.0, binning = 2 spot = 7 camera = 3 CCDERASER: Bad points replaced with interpolated values 26-Apr-13 17:17:47 Tapering will be done over 19 pixels NEW image file on unit 2 : WTI042413_1series4.ali section input min&max output min&max & mean 0 -101.73 948.25 -101.73 948.25 247.48 1 -49.30 914.35 -49.30 914.35 239.89 2 -69.46 903.93 -69.46 903.93 237.23 3 -53.62 852.71 -53.62 852.71 243.79 4 -71.73 885.86 -71.73 885.86 231.84 5 -49.56 848.08 -49.56 848.08 234.89 6 -46.49 800.82 -46.49 800.82 228.72 7 -61.24 808.35 -61.24 808.35 224.79 8 -71.49 797.73 -71.49 797.73 223.97 9 -64.88 759.71 -64.88 759.71 223.50 10 -48.23 779.95 -48.23 779.95 225.10 11 -62.12 749.87 -62.12 749.87 220.20 12 -46.34 696.07 -46.34 696.07 227.50 13 -67.06 727.07 -67.06 727.07 214.51 14 -60.51 676.21 -60.51 676.21 216.83 15 -49.64 708.32 -49.64 708.32 215.04 16 -40.29 735.08 -40.29 735.08 213.07 17 -112.54 660.57 -112.54 660.57 210.62 18 -46.72 674.80 -46.72 674.80 208.46 19 -44.16 671.09 -44.16 671.09 207.75 20 -35.91 676.56 -35.91 676.56 206.88 21 -52.77 646.01 -52.77 646.01 204.47 22 -37.42 652.81 -37.42 652.81 205.09 23 -48.60 685.61 -48.60 685.61 202.61 24 -51.53 629.78 -51.53 629.78 201.78 25 -45.66 631.28 -45.66 631.28 209.34 26 -45.03 672.54 -45.03 672.54 202.18 27 -50.60 616.17 -50.60 616.17 200.40 28 -39.01 658.18 -39.01 658.18 201.31 29 -40.01 666.36 -40.01 666.36 206.21 30 -32.56 680.30 -32.56 680.30 201.96 31 -47.82 632.59 -47.82 632.59 204.10 32 -42.33 601.39 -42.33 601.39 206.52 33 -52.43 638.68 -52.43 638.68 202.29 34 -52.68 702.70 -52.68 702.70 204.04 35 -50.72 627.16 -50.72 627.16 205.22 36 -50.01 667.98 -50.01 667.98 206.01 37 -50.64 655.24 -50.64 655.24 205.43 38 -54.16 652.54 -54.16 652.54 207.98 39 -38.65 672.86 -38.65 672.86 209.26 40 -35.28 711.90 -35.28 711.90 211.34 41 -68.00 692.75 -68.00 692.75 212.67 42 -40.54 688.03 -40.54 688.03 215.73 43 -49.61 655.11 -49.61 655.11 217.73 44 -40.91 732.34 -40.91 732.34 219.78 45 -49.18 729.53 -49.18 729.53 223.05 46 -44.92 730.61 -44.92 730.61 225.91 47 -44.63 725.70 -44.63 725.70 226.74 48 -38.93 717.03 -38.93 717.03 231.15 49 -39.54 775.28 -39.54 775.28 233.25 50 -43.99 737.69 -43.99 737.69 237.59 51 -43.87 767.38 -43.87 767.38 241.58 52 -59.50 808.51 -59.50 808.51 242.61 53 -36.41 816.13 -36.41 816.13 246.91 54 -46.98 804.17 -46.98 804.17 257.74 55 -52.60 785.58 -52.60 785.58 247.84 56 -49.44 939.40 -49.44 939.40 257.30 57 -58.23 914.71 -58.23 914.71 264.54 58 -51.82 892.13 -51.82 892.13 274.37 59 -52.40 944.49 -52.40 944.49 280.72 60 -40.80 1011.32 -40.80 1011.32 282.30 SUCCESSFULLY COMPLETED