*** Entries to program findbeads3d *** InputFile = WTI042413_1series4_3dfind.rec OutputFile = WTI042413_1series4_3dfind.mod BeadSize = 14.84 BinningOfVolume = 3 MinRelativeStrength = 0.05 StorageThreshold = 0 MinSpacing = 0.9 *** End of entries *** RO image file on unit 1 : WTI042413_1series4_3dfind.rec Size= 86810 K Number of columns, rows, sections ..... 618 116 620 Map mode .............................. 1 (16-bit integer) Start cols, rows, sects, grid x,y,z ... 0 0 0 618 116 620 Pixel spacing (Angstroms).............. 20.22 20.22 20.22 Cell angles ........................... 90.000 90.000 90.000 Fast, medium, slow axes ............... X Y Z Origin on x,y,z ....................... 6.740 1234. 13.48 Minimum density ....................... -17529. Maximum density ....................... 14661. Mean density .......................... 335.17 tilt angles (original,current) ........ 0.0 0.0 0.0 90.0 0.0 0.0 Space group,# extra bytes,idtype,lens . 0 0 0 0 5 Titles : SerialEM: Digitized on Janelia Krios 26-Apr-13 14:20:21 Tilt axis angle = 168.0, binning = 2 spot = 7 camera = 3 CCDERASER: Bad points replaced with interpolated values 26-Apr-13 17:17:47 NEWSTACK: Images copied, transformed 30-Apr-13 13:19:44 TILT: Tomographic reconstruction 30-Apr-13 13:20:03 16571 candidate peaks found 10762 candidate peaks left after eliminating close points Histogram smoothed with H = 0.200 has dip at 0.3311, peaks at 0.0386 and 0.4868 Histogram smoothed with H = 0.05 has lowest dip at 0.3085 115 peaks being averaged to make reference for correlation 1165 peaks found by correlation 1021 peaks left after eliminating close points Histogram smoothed with H = 0.200 has dip at 0.3356, peaks at 0.0657 and 0.7964 Histogram smoothed with H = 0.05 has lowest dip at 0.3582 148 peaks are above the histogram dip Storing an additional 148 peaks in model down to a value of 0.0043 SUCCESSFULLY COMPLETED