# Command file for running BEADTRACK # ####CreatedVersion#### 3.12.24 # # For beads lighter than background, add a line with "LightBeads" # # To restrict tilt alignment to a subset of views, add a line with: # "MaxViewsInAlign #_of_views" # # To exclude views, add a line "SkipViews view_list" with the list of views # # To specify sets of views to be grouped separately in automapping, add a line # "SeparateGroup view_list" with the list of views, one line per group # $beadtrack -StandardInput SobelFilterCentering KernelSigmaForSobel 1.5 ImageFile MTEg5series6D.preali InputSeedModel MTEg5series6D.seed OutputModel MTEg5series6D.fid ImagesAreBinned 1 PrealignTransformFile MTEg5series6D.prexg RotationAngle -3.3 TiltFile MTEg5series6D.rawtlt TiltDefaultGrouping 7 MagDefaultGrouping 5 RotDefaultGrouping 1 BeadDiameter 9.43 FillGaps MaxGapSize 5 RoundsOfTracking 4 # # Set this to 1 to track in local areas LocalAreaTracking 1 LocalAreaTargetSize 1000 MinBeadsInArea 8 MinOverlapBeads 5 # # CONTROL PARAMETERS FOR EXPERTS, EXPERIMENTATION, OR SPECIAL CASES # # minimum range of tilt angles for finding axis and for finding tilts MinViewsForTiltalign 4 MinTiltRangeToFindAxis 10.0 MinTiltRangeToFindAngles 20.0 BoxSizeXandY 40,40 MaxBeadsToAverage 4 # points and minimum for extrapolation PointsToFitMaxAndMin 7,3 # fraction of mean, and # of SD below mean: density criterion for rescue DensityRescueFractionAndSD 0.6,1.0 # distance criterion for rescue DistanceRescueCriterion 10.0 # relaxation of criterion for density and distance rescues RescueRelaxationDensityAndDistance 0.7,0.9 # distance for rescue after fit PostFitRescueResidual 2.5 # relaxation of density criterion, maximum radius to search DensityRelaxationPostFit 0.9 MaxRescueDistance 2.5 # Max and min residual changes to use to get mean and SD change ResidualsToAnalyzeMaxAndMin 9,5 # minimum residual difference, criterion # of sd's DeletionCriterionMinAndSD 0.04,2.0 $if (-e ./savework) ./savework