*** Entries to program tilt *** InputProjections = MTEg5series6D.ali OutputFile = MTEg5series6D_full.rec ActionIfGPUFails = 2,2 AdjustOrigin = 1 EXCLUDELIST2 = 1 FULLIMAGE = 4096 3072 IMAGEBINNED = 1 LOG = 0.0 MODE = 1 OFFSET = 0.0 PERPENDICULAR = 1 RADIAL = 0.35 0.05 SCALE = 0.0 250.0 SHIFT = 0.0 0.0 SLICE = -1 -1 SUBSETSTART = 0 0 THICKNESS = 300 TILTFILE = MTEg5series6D.tlt TOTALSLICES = 0 3071 UseGPU = 0 XAXISTILT = -2.07 XTILTFILE = MTEg5series6D.xtilt *** End of entries *** RO image file on unit 1 : MTEg5series6D.ali Size= 1499137 K Number of columns, rows, sections ..... 4096 3072 61 Map mode .............................. 1 (16-bit integer) Start cols, rows, sects, grid x,y,z ... 0 0 0 4096 3072 61 Pixel spacing (Angstroms).............. 5.300 5.300 5.300 Cell angles ........................... 90.000 90.000 90.000 Fast, medium, slow axes ............... X Y Z Origin on x,y,z ....................... 0.000 0.000 0.000 Minimum density ....................... 22.000 Maximum density ....................... 971.00 Mean density .......................... 377.60 tilt angles (original,current) ........ 0.0 0.0 0.0 0.0 0.0 0.0 Space group,# extra bytes,idtype,lens . 0 0 0 0 6 Titles : SerialEM: Boulder 3DEM TF20 16-Oct-13 16:00:27 Tilt axis angle = -3.3, binning = 1 spot = 6 camera = 1 CCDERASER: Bad points replaced with interpolated values 21-Oct-13 14:34:36 NEWSTACK: Images copied, transformed 5-Nov-13 14:18:16 ctfPhaseFlip: CTF correction with phase flipping only 05-Nov-13 14:18:53 CCDERASER: Bad points replaced with interpolated values 5-Nov-13 14:27:00 Radial weighting function parameters IRMAX = 1433 IWIDE = 204 Output map densities incremented by 0.00 and then multiplied by 250.00 Output map is sectioned perpendicular to the tilt axis Data mode of output file is 1 Taking logarithm of input data plus 0.000 FORMATTED ro file opened: /home/NOBACKUP/mast/MTEg5_101013/MTEg5series6D/MTEg5series6D.tlt FORMATTED ro file opened: /home/NOBACKUP/mast/MTEg5_101013/MTEg5series6D/MTEg5series6D.xtilt Constant alpha tilt of 0.0 to be applied based on angles from file Global alpha tilt of -2.1 will be applied ------------------------------------------------------------------------------ Rows 0 to 0, (at intervals of 1) of the projection planes will be reconstructed. Thickness of reconstructed slice is 300 pixels. Full aligned stack will be assumed to be 4096 by 3072 pixels Aligned stack will be assumed to be a subset starting at 0 0 Computed slices are part of a total volume from slice 1 to 3072 Tilt angles were entered from a tilt file NEW image file on unit 2 : MTEg5series6D_full.rec Projection angles: -58.95 -56.95 -54.95 -52.95 -50.94 -48.94 -46.93 -44.93 -42.90 -40.88 -38.86 -36.83 -34.81 -32.79 -30.76 -28.74 -26.72 -24.70 -22.69 -20.68 -18.66 -16.65 -14.64 -12.63 -10.65 -8.66 -6.68 -4.69 -2.71 -0.73 1.23 3.19 5.15 7.11 9.07 11.03 12.99 15.00 17.02 19.03 21.04 23.05 25.07 27.06 29.06 31.05 33.05 35.05 37.03 39.00 40.98 42.96 44.94 46.94 48.94 50.93 52.93 54.92 56.91 58.90 ------------------------------------------------------------------------------ Exiting after setting up output file for chunk writing SUCCESSFULLY COMPLETED CHUNK DONE