# THIS IS A COMMAND FILE TO MAKE 3 TOMOGRAM SAMPLES # ####CreatedVersion#### 3.8.25 # # The sample aligned stacks will each be 60 pixels in Y # The sample tomograms will have 10 slices # To get more slices in the samples, change "sampletilt 25 34" by # decreasing 25 and increasing 34 by equal amounts, up to 0 and 59 # # THESE ARE COMMANDS TO MAKE AN ALIGNED STACK FROM THE ORIGINAL STACK # ####CreatedVersion#### 4.6.18 # # It assumes that the views are in order in the image stack # # The size argument should be ,, for the full area or specify the desired # size (e.g.: ,10) # # The offset argument should be 0,0 for no offset, 0,300 to take an area # 300 pixels above the center, etc. # $newstack -StandardInput InputFile MTEg5series6D.st OutputFile MTEg5series6D.ali TransformFile MTEg5series6D.xf SizeToOutputInXandY ,60 OffsetsInXandY 0,0 #DistortionField .idf ImagesAreBinned 1 #GradientFile MTEg5series6D.maggrad $sampletilt 25 34 1506 MTEg5series6D mid.rec tilt.com # THESE ARE COMMANDS TO MAKE AN ALIGNED STACK FROM THE ORIGINAL STACK # ####CreatedVersion#### 4.6.18 # # It assumes that the views are in order in the image stack # # The size argument should be ,, for the full area or specify the desired # size (e.g.: ,10) # # The offset argument should be 0,0 for no offset, 0,300 to take an area # 300 pixels above the center, etc. # $newstack -StandardInput InputFile MTEg5series6D.st OutputFile MTEg5series6D.ali TransformFile MTEg5series6D.xf SizeToOutputInXandY ,60 OffsetsInXandY 0,1042 #DistortionField .idf ImagesAreBinned 1 #GradientFile MTEg5series6D.maggrad $sampletilt 25 34 2548 MTEg5series6D top.rec tilt.com # THESE ARE COMMANDS TO MAKE AN ALIGNED STACK FROM THE ORIGINAL STACK # ####CreatedVersion#### 4.6.18 # # It assumes that the views are in order in the image stack # # The size argument should be ,, for the full area or specify the desired # size (e.g.: ,10) # # The offset argument should be 0,0 for no offset, 0,300 to take an area # 300 pixels above the center, etc. # $newstack -StandardInput InputFile MTEg5series6D.st OutputFile MTEg5series6D.ali TransformFile MTEg5series6D.xf SizeToOutputInXandY ,60 OffsetsInXandY 0,-1042 #DistortionField .idf ImagesAreBinned 1 #GradientFile MTEg5series6D.maggrad $sampletilt 25 34 464 MTEg5series6D bot.rec tilt.com $if (-e ./savework) ./savework