# command file to run ctfplotter # ####CreatedVersion#### 3.12.20 # # $${IMOD_DIR}/ctfplotter/ctfplotter -Stand # InputStack MTEg5series6D.st # # The tilt angle file - .rawtlt could be used instead if .tlt not available yet AngleFile MTEg5series6D.tlt DefocusFile MTEg5series6D.defocus # # How many degrees the tilt axis deviates from vertical (Y axis) (CCW positive) AxisAngle -3.3 # # Image pixel size in nanometers PixelSize 0.53 # # Expected defocus at the tilt axis in nanometers (underfocus is positive) ExpectedDefocus 6000.0 # # Starting and ending tilt angles for initial analysis AngleRange -20.0 20.0 # # Microscope voltage in kV Voltage 200 # # Microscope spherical aberration in millimeters SphericalAberration 2.0 # # Fraction of amplitude contrast AmplitudeContrast 0.07 # # Defocus tolerance in nanometers defining the center strips DefocusTol 200 PSResolution 101 TileSize 256 LeftDefTol 2000.0 RightDefTol 2000.0 ConfigFile /usr/local/ImodCalib/CTFnoise/DE-12Test/DE-12Div2.cfg $if (-e ./savework) ./savework