Description of PEET
PEET (Particle Estimation for Electron Tomography) is a package
for aligning and averaging particles in 3-D volumes extracted from tomograms.
It finds the optimal alignment of
each particle with a reference volume through several iterations.
If PEET and IMOD are both installed, most PEET operations are available from
the eTomo graphical user interface in IMOD.
PEET uses the parallel processing framework within IMOD, so that the lengthy
be distributed to multiple cores on one computer, to a set of linked
workstations, or to a cluster.
It is written
in Matlab and a compiled version is distributed along with the Matlab runtime
environment needed to run it.
was first applied for averaging axonemes and its methods were described in:
Nicastro, D., C. Schwartz, J. Pierson, R. Gaudette, M.E. Porter and J.R. McIntosh (2006) The molecular architecture of axonemes revealed by cryoelectron tomography. Science. 313: 944-948.
Please cite this paper if you use PEET in your research. The package has developed considerably since, with applications to isolated particles as well as ones along fibers. Some recent applications are described in:
Xiong, Q., M. K. Morphew, C. L. Schwartz, A. H. Hoenger, D. N. Mastronarde (2009) CTF determination and correction for low dose tomographic tilt series. J. Struct. Biol. 168: 378-87.
Cope, J., S. Gilbert, I. Rayment, D. Mastronarde, A. Hoenger (2010) Cryo-electron tomography of microtubule-kinesin motor complexes. J. Struct. Biol. 170: 257-65.
PEET Discussion List
The PEET discussion listserver provides a means for users
to ask questions and share their experiences, as well as
a way to receive notification of new releases and other news.
The list is not moderated, but joining requires approval.
To subscribe to this list, send a message to listproc@lists.Colorado.EDU with the contents of your message being:
subscribe peet-list your-full-name
where your-full-name is your first name followed by your last name, including any punctuation and spaces. After your request is approved, you will be added to the list and will receive an email with more instructions on using the list.
To post to the list, send an email (plain text, not html) addressed to firstname.lastname@example.org.
To remove yourself from the list, send a message to listproc@lists.Colorado.EDU with the contents of the message being:
To access the list archives, see these instructions.
Documentation for PEET
Man pages are available in both ASCII and html formats for each of the
programs comprising PEET. These pages ship with PEET, and can also be
or downloaded from the "man" subdirectory of
A guide for running PEET through the eTomo graphical user interface can be
found here. This guide is distributed in IMOD
and can be opened from the Help menu in eTomo once PEET has been installed.
A growing set of introductory tutorials can be downloaded from
or viewed on our YouTube channel at
The associated sample data sets can be downloaded from
Submitting a Bug or Requesting Help
Defect submissions and requests for help are always welcome.
They can be sent to the discussion list or emailed
We try to provide excellent responsiveness and customer service.
You can help considerably by making sure your requests are clear and
complete. Before submitting, please consider the following. 1) Have you
updated to the most recent supported versions of PEET and IMOD and
verified that the problem still exists there? If not, please do so
before submitting. Considerable time and effort are wasted repeatedly
tracking down bugs which already been fixed.
2) Please be as explicit as possible. Describe exactly what you were
doing, what the problem you encountered was, and, if possible, how to
reproduce it. At a minimum, always include a description of the
hardware you are running on, the operating system name and version,
and both PEET and IMOD versions.
please include an tomosnapshot (File / Run Tomosnapshot) taken in the
PEET project directory immediately after the error occured. Screen shots
can be included if desired, but are rarely helpful; a tomosnapshot is
more likely to contain the necessary information.